猪链球菌rpoE基因克隆及生物信息学分析

doi:10.3969/j.issn.1004-1524.2020.07.02

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (7): 1149-1154.DOI: 10.3969/j.issn.1004-1524.2020.07.02

猪链球菌rpoE基因克隆及生物信息学分析

杜炎斌, 张港琛, 王瑜欣, 刘宝宝, 宫胜龙, 东笑, 汪洋^*

河南科技大学动物科技学院洛阳市畜禽分子病原与免疫学重点实验室,河南洛阳 471003

收稿日期:2019-10-23 出版日期:2020-07-25 发布日期:2020-07-28
通讯作者: *汪洋,E-mail:wangyocean@163.com
作者简介:杜炎斌(1996—),男,甘肃平凉人,硕士研究生,主要从事兽医微生物及免疫学研究。E-mail:951611332@qq.com
基金资助:
国家自然科学基金(31772761); 河南省自然科学基金(182300410047)

Cloning and bioinformatic analysis of rpoE gene of Streptococcus suis

DU Yanbin, ZHANG Gangchen, WANG Yuxin, LIU Baobao, GONG Shenglong, DONG Xiao, WANG Yang^*

Key Laboratory of Molecular Pathogen and Immunology of Animal of Luoyang, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China

Received:2019-10-23 Online:2020-07-25 Published:2020-07-28

摘要/Abstract

摘要： 本研究旨在克隆猪链球菌rpoE基因,并通过生物信息学软件预测分析猪链球菌rpoE基因编码蛋白功能及其调节机制。以猪链球菌基因组为模板扩增rpoE基因,成功测序后通过生物信息学软件预测该基因编码蛋白的理化性质、抗原表位等。结果表明,成功克隆的猪链球菌rpoE基因全长582 bp,编码一种富含谷氨酸、天冬氨酸及丝氨酸的亲水性胞质蛋白;二级结构分析显示,RpoE蛋白主要结构元件为α-螺旋和无规则卷曲,分别占52.3%和36.3%。综合分析认为,RpoE蛋白上存在5个优势抗原表位。

关键词: 猪链球菌, RpoE, 克隆, 生物信息学

Abstract: This study aimed to clone the rpoE gene of Streptococcus suis and to analyze the function and related biological characteristics of the rpoE gene encoding protein of Streptococcus suis by bioinformatic software. The rpoE gene was amplified from Streptococcus suis and sequenced; the physicochemical properties and antigenic epitopes of the protein encoded by the gene were predicted by bioinformatic software. The results showed that the rpoE gene of Streptococcus suis was 582 bp in length, encoding a hydrophilic cytoplasmic protein rich in glutamic acid, aspartic acid and serine. Besides, the secondary structure analysis showed that the main structural components of RpoE protein were α-helix and random coil, accounting for 52.3% and 36.3%, respectively. The comprehensive analysis indicates that there are 5 dominant epitopes on RpoE protein.

Key words: Streptococcus suis, RpoE, clone, bioinformatic analysis

中图分类号:

S855.1⁺1

杜炎斌, 张港琛, 王瑜欣, 刘宝宝, 宫胜龙, 东笑, 汪洋. 猪链球菌rpoE基因克隆及生物信息学分析[J]. 浙江农业学报, 2020, 32(7): 1149-1154.

DU Yanbin, ZHANG Gangchen, WANG Yuxin, LIU Baobao, GONG Shenglong, DONG Xiao, WANG Yang. Cloning and bioinformatic analysis of rpoE gene of Streptococcus suis[J]. , 2020, 32(7): 1149-1154.

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猪链球菌rpoE基因克隆及生物信息学分析

Cloning and bioinformatic analysis of rpoE gene of Streptococcus suis

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