滇牡丹中3个类查尔酮合成酶基因的克隆与表达

doi:10.3969/j.issn.1004-1524.2019.09.11

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (9): 1478-1484.DOI: 10.3969/j.issn.1004-1524.2019.09.11

滇牡丹中3个类查尔酮合成酶基因的克隆与表达

原晓龙, 李云琴, 王毅^*

云南省林业科学院云南省森林植物培育与开发利用重点实验室,国家林业和草原局云南珍稀濒特森林植物保护和繁育重点实验室,云南昆明 650201

收稿日期:2019-04-01 出版日期:2019-09-25 发布日期:2019-10-11
通讯作者: *王毅,E-mail: 22825818@qq.com
作者简介:原晓龙(1986—),男,陕西蒲城人,硕士,助理研究员,主要从事林木育种和分子生物学研究。E-mail: xiaolony@126.com
基金资助:
国家自然科学基金(31860177); 云南省应用基础研究青年项目(2016FD100); 云南省林业科学院创新基金(QN2018-01)

Cloning and expression of three chalcone synthase-like genes from Paeonia delavayi

YUAN Xiaolong, LI Yunqin, WANG Yi^*

Yunnan Academy of Forestry, Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants, Conservation of Rare, Endangered & Endemic Forest Plants, Public Key Laboratory of the National Forestry and Grassland Administration, Kunming 650201, China

Received:2019-04-01 Online:2019-09-25 Published:2019-10-11

摘要/Abstract

摘要： 从开黄花的滇牡丹转录组中分离了3个CHS基因,利用生物信息学预测其基因功能,并比较不同花发育时期CHS基因的表达量。结果显示:3个CHS基因的cDNA全长分别为1 173、1 185、1 128 bp,依次命名为PdCHS1(GenBank登录号MK516264)、PdCHS2(GenBank登录号MK516265)和PdCHS3(GenBank登录号MK516266),分别编码390、394和375个氨基酸;这3个CHS基因编码的蛋白均为无信号肽的非分泌蛋白,均含查尔酮合成酶活性位点基序(G/A) FGPG。聚类结果显示,滇牡丹中的3个CHS蛋白归属于不同分支。基因表达结果显示,PdCHS1基因在花蕾期和花蕊中表达量较高,PdCHS2基因在末花期和初花期表达量较高,PdCHS3基因在末花期和花蕾期表达量较高。这3个CHS基因分别参与不同次生代谢产物的合成,推测PdCHS1参与柚皮素查尔酮,PdCHS2参与芪类化合物,PdCHS3参与聚酮类化合物的生物合成。

关键词: 滇牡丹, 查尔酮合成酶基因, 生物信息学, 次生代谢

Abstract: Three CHS genes were isolated from the transcriptome of wild Paeonia delavayi with yellow flower, gene functions were predicted by using bioinformatics analysis, and gene expression levels in different developmental period of flowers were also analyzed. The results showed that the full-length cDNA of these three CHS genes were 1 173, 1 185, 1128 bp, respectively. They were named as PdCHS1 (GenBank ID was MK516264), PdCHS2 (GenBank ID was MK516265) and PdCHS3 (GenBank ID was MK516266), and encoded 390, 394 and 375 aa, respectively. The proteins encoded by these three CHS genes were non-secreted proteins and had no signal peptide, they all contained highly conserved catalytic active motif (G/A) FGPG. Phylogenic analysis indicated that these 3 proteins belonged to different branches. Expression level of PdCHS1 was high in the stamina and budding stage, expression level of PdCHS2 was high in the blossom falling stage and initial flowering stage, and expression level of PdCHS3 was high in the blossom falling stage and budding stage. These three CHS genes could participate in the different biosynthesis of secondary metabolites, PdCHS1 might relate to the naringenin biosynthesis, PdCHS2 might take part in the stibenoid biosynthesis, and PdCHS3 might involve in the polyketide biosynthesis.

Key words: Paeonia delavayi, chalcone synthase gene, bioinformatics, biosynthetic pathways of metabolism

中图分类号:

S567.1⁺5

原晓龙, 李云琴, 王毅. 滇牡丹中3个类查尔酮合成酶基因的克隆与表达[J]. 浙江农业学报, 2019, 31(9): 1478-1484.

YUAN Xiaolong, LI Yunqin, WANG Yi. Cloning and expression of three chalcone synthase-like genes from Paeonia delavayi[J]. , 2019, 31(9): 1478-1484.

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滇牡丹中3个类查尔酮合成酶基因的克隆与表达

Cloning and expression of three chalcone synthase-like genes from Paeonia delavayi

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