零陵红衣葱葱油树脂的提取与分析

浙江农业学报

零陵红衣葱葱油树脂的提取与分析

（1.湖南科技学院化学与生物工程学院，湖南永州 425199； 2.湖南科技学院湘南优势植物资源综合利用湖南省重点实验室，湖南永州 425199； 3.湖南省食品质量监督检验研究院，湖南长沙 410111）

出版日期:2016-08-25 发布日期:2016-08-04

Study on the extraction and analysis of red onion oleoresin

（1. Department of Biochemistry， Hunan University of Science and Engineering， Yongzhou 425199， China; 2. Key Laboratory of Comprehensive Utilization of Advantage Plants Resources in Hunan South， Hunan University of Science and Engineering， Yongzhou 425199， China; 3. Hunan Institute of Food Quality Supervision Inspection and Research， Changsha 410111， China）

Online:2016-08-25 Published:2016-08-04

摘要/Abstract

摘要： 以零陵红衣葱为原料，探讨不同萃取剂、料液比、时间、温度、提取次数对葱油树脂产品得率的影响，并通过气相色谱—质谱（GCMS）对零陵红衣葱葱油树脂成分进行定性分析，用峰面积归一化法检测各化合物的相对百分含量。结果表明，零陵红衣葱葱油树脂最适提取工艺条件为：以石油醚为萃取剂，提取温度47 ℃、提取时间100 min、料液比1∶8、提取2次。在此条件下，零陵红衣葱葱油树脂的产品得率为0638 4%。在零陵红衣葱葱油树脂样品中共检测出18种挥发性化合物成分，占其色谱流出总量的9781%，其中，含硫化合物占383%，酸类占2447%，酮类占271%，醇类占1706%，酯类占1081%，醛类占036%，包括二甲基二硫醚、1，3二噻烷、己醇、二十烯醇、2甲基2戊醇、3羟基2丁酮、5，7二甲基色酮3甲醛、棕榈酸、十八二烯酸、十八烯酸、邻苯二甲酸二辛酯。

关键词: GCMS, 零陵红衣葱, 葱油树脂

Abstract: In the present study， Lingling red onion was taken as raw material. Different extraction solvents， solidliquid ratio， reaction time， reaction temperature and extraction times were employed to examine the influences on extraction yield of oleoresin. The chemical constituents were separated and identified by gas chromatographymass spectrometry （GCMS）， and the relative contents in percentage of compounds were determined by peak area normalization method. It was shown that the optimal extraction conditions were as follows: petroleum ether as extraction solvent， reaction temperature of 47 ℃， reaction time of 100 min， solidliquid ratio of 1∶8 and extraction times of twice. Under this conditions， the extraction rate could reach 0638 4% from 50 g Lingling red onion. A total of 18 volatile compounds were separated， and they accounted for 9781% of the total extractions， in which， sulfurcontaining compounds were 383%， acids were 2447%， ketones were 271%， alcohols were 1706%， esters were 1081% and aldehydes were 036%. The constituents included dimethyl disulfide， 1，3dithiane， hexanol， gadoleyl alcohol， 2methyl2pentanol， 3hydroxy2butanone， 5，7dimethylchromone3carboxaldehyde， palmitic acid， octadecadienoic acid， octadecenoate， dioctyl phthalate.

Key words: GCMS, Lingling red onion, onion oleoresin

刘小文1，2，盘俊1，陈雄3，全沁果1，敖艳1，3，胡莎1，段丽萍1，齐成媚1，何福林1，2，. 零陵红衣葱葱油树脂的提取与分析[J]. 浙江农业学报.

LIU Xiaowen1，2， PAN Jun1， CHEN Xiong3， QUAN Qinguo1， AO Yan1，3， HU Sha1， DUAN Liping1， QI Chengmei1， HE Fulin1，2， YUAN Zhihui1，2，*. Study on the extraction and analysis of red onion oleoresin[J]. .