天然柠檬醛衍生物对食品腐败细菌的抑制活性

doi:10.3969/j.issn.1004-1524.2016.11.19

浙江农业学报 ›› 2016, Vol. 28 ›› Issue (11): 1928-1933.DOI: 10.3969/j.issn.1004-1524.2016.11.19

天然柠檬醛衍生物对食品腐败细菌的抑制活性

李兆双^{1, 2}, 王喜男^{1, 2}, 王鹏^{1, 2}, 陈尚钘^{1, 2*, *}, 范国荣^{1, 2}, 王宗德^{1, 2}

1.江西农业大学林学院,国家林业局江西省樟树工程技术研究中心,江西南昌 330045;
2.江西农业大学江西特色林木资源培育与利用2011协同创新中心,江西南昌330045

收稿日期:2016-05-31 出版日期:2016-11-15 发布日期:2016-11-16
通讯作者: 陈尚钘,E-mail:csxing@126.com
作者简介:李兆双(1993—),男,江西萍乡人,硕士,主要从事林产资源加工研究。E-mail:724059421@qq.com
基金资助:
国家自然科学基金项目(31360163); 中央财政林业科技推广示范项目(JXTG [2014]-10); 江西省林业科技创新项目(201502)

Antimicrobial activity of natural citral derivatives on food deterioration bacteria

LI Zhao-shuang^{1, 2}, WANG Xi-nan^{1, 2}, WANG Peng^{1, 2}, CHEN Shang-xing^{1, 2, *}, FAN Guo-rong^{1, 2}, WANG Zong-de^{1, 2}

1. College of Forestry, Jiangxi Agricultural University, Camphor Tree Engineering and Technology Research Center for State Forestry Bureau and Jiangxi Province, Nanchang 330045, China;
2. 2011 Collaborative Innovation Center of Jiangxi Typical Trees Cultivation and Utilization, Jiangxi Agricultural University, Nanchang 330045, China

Received:2016-05-31 Online:2016-11-15 Published:2016-11-16

摘要/Abstract

摘要： 采用抑菌圈法和琼脂二倍稀释法,测试了12种天然柠檬醛衍生化合物对大肠埃希菌、金黄色葡萄球菌、表皮葡萄球菌、绿脓杆菌的抑菌活性。结果表明,12种天然柠檬醛衍生化合物对4种细菌均有一定的抑菌效果。其中,异丁酸香叶酯对大肠埃希菌抑菌效果最佳,抑菌圈直径为14.83 mm,最低抑菌浓度为8 mg·L^-1;橙花醇对金黄色葡萄球菌及表皮葡萄球菌抑制效果最佳,抑菌圈直径分别为15.80和14.33 mm,最低抑菌浓度分别为8和16 mg·L^-1;香叶醇对绿脓杆菌抑制效果最佳,抑菌圈直径为15.00 mm,最低抑菌浓度为16 mg·L^-1。经天然柠檬醛衍生物处理后,食品腐败细菌细胞膜的相对渗透率升高,表明这些化合物可能对细菌细胞膜具有破坏作用。

关键词: 天然柠檬醛衍生物, 食品腐败细菌, 抑菌活性

Abstract: The antibacterial activities of 12 natural citral derivatives against Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa were evaluated through anti-bacterium-circles and two-fold agar dilution methods. The results showed that these citral derivatives all had antibacterial effect on four bacteria. Geranyl isobutyrate exhibited the strongest effect on E. coli, with the diameter of antibacterial circle 14.83 mm and MIC 8 mg·L^-1; Nerol strongest on S. aureus and S. epidermidis, with the diameters of antibacterial circle 15.80 and 14.33 mm and the MIC 8 and 16 mg·L^-1; Geraniol best on P. aeruginosa, with the diameter of antibacterial circle 15.00 mm and MIC 16 mg·L^-1. The relative permeability of bacterial’s cytomembrane was measured, and the result showed that the relative permeability increased after treated by the citral derivatives, which implied that these citral derivatives had a destructive effect on the bacterial cytomembrane.

Key words: natural citral derivatives, food deterioration bacteria, antimicrobial activity

中图分类号:

S917.4

李兆双, 王喜男, 王鹏, 陈尚钘, 范国荣, 王宗德. 天然柠檬醛衍生物对食品腐败细菌的抑制活性[J]. 浙江农业学报, 2016, 28(11): 1928-1933.

LI Zhao-shuang, WANG Xi-nan, WANG Peng, CHEN Shang-xing, FAN Guo-rong, WANG Zong-de. Antimicrobial activity of natural citral derivatives on food deterioration bacteria[J]. , 2016, 28(11): 1928-1933.

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天然柠檬醛衍生物对食品腐败细菌的抑制活性

Antimicrobial activity of natural citral derivatives on food deterioration bacteria

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