性成熟前期尼罗罗非鱼雌雄性别生长差异与性类固醇激素水平比较

doi:10.3969/j.issn.1004-1524.2016.10.08

浙江农业学报 ›› 2016, Vol. 28 ›› Issue (10): 1678-1686.DOI: 10.3969/j.issn.1004-1524.2016.10.08

性成熟前期尼罗罗非鱼雌雄性别生长差异与性类固醇激素水平比较

岳蒙蒙, 赵金良^*, 唐首杰, 王燕, 赵永华

上海海洋大学农业部淡水水产种质资源重点实验室,上海 201306

收稿日期:2016-01-22 出版日期:2016-10-15 发布日期:2016-11-20
通讯作者: 赵金良, E-mail:jlzhao@shou.edu.cn
作者简介:岳蒙蒙(1990—),女,河南郑州人,博士研究生,从事水产动物遗传育种与繁殖研究。E-mail:1534451302@qq.com
基金资助:
国家现代农业产业技术体系专项资金(CARS-49); 水产动物遗传育种中心上海市协同创新中心(ZF1206)

Study on growth difference and steroid hormones between female and male Nile tilapia Oreochromis niloticus before sexual maturity

YUE Meng-meng, ZHAO Jin-liang^*, TANG Shou-jie, WANG Yan, ZHAO Yong-hua

Laboratory of Freshwater Fisheries Germplasm Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China

Received:2016-01-22 Online:2016-10-15 Published:2016-11-20

摘要/Abstract

摘要： 利用分子标记法、醋酸洋红压片法、组织切片技术等方法进行个体性别鉴定,比较了性成熟前期(0.5~4.0月龄)尼罗罗非鱼雌、雄鱼生长差异,并检测了2.5~4.0月龄雌、雄鱼血清睾酮(T)、雌二醇(E₂)激素含量。结果发现,雌、雄鱼全长、体质量分别自3.0月龄、2.5月龄开始出现差异显著(P<0.05)。Logistic拟合全长、体质量生长方程为:L_t(♀)=16.443/(1+e^{(2.821-1.363*}^t⁾),L_t(♂)=18.248/(1+e^{(2.752-1.295*}^t⁾);W_t(♀)=107.704/(1+e^{(5.157-1.464*}^t⁾),W_t(♂)=134.796/(1+e^{(5.080-1.417*}^t⁾),雌、雄鱼体质量生长拐点分别为3.523、3.585月龄,拐点体质量分别为52.352、67.398 g。雄鱼全长、体质量生长速度始终大于雌鱼,雄鱼快速增长期区间长度、体质量增加量大于雌鱼。2.5月龄之后,雌、雄鱼E₂含量差异显著(P<0.05),3.0月龄之后,雌、雄鱼T含量差异显著(P<0.05),推测E₂、T含量差异是引起尼罗罗非鱼雌雄性别生长差异的原因之一。

关键词: 尼罗罗非鱼, 雌雄性别, 性类固醇激素

Abstract: Studies were conducted to reveal the growth differences between female and male Nile tilapia (Oreochromis niloticus) by identifying the gender with molecular marker, acid red dyeing method and histological section, meanwhile, the content of serum steroid hormones, testosterone (T) and estradiol (E₂), were also investigated. Results showed that the total length, body weight in the female and male began to show significant differences (P <0.05) at 3.0 and 2.5 months age, respectively. Simultaneously, the Logistic growth equation can be expressed as L_t(♀)=16.443/(1+e^{(2.821-1.363*}^t⁾), L_t(♂)=18.248/(1+e^(2.72-1.295*^t⁾), W_t(♀)=107.704/(1+e^{(5.157-1.464*}^t⁾), W_t(♂)=134.796/(1+e^{(5.080-1.417*}^t⁾). The inflection point for the body weight growth was 3.523 month in the female and 3.585 month in the male, and the corresponding weight was 52.352 and 67.398 g, respectively. The growth rate of total length and body weight in the male was throughout larger than that in the female. Furthermore, the male showed longer period and heavier increment than the female in the fast-growing period. After 3.0 months age, the content of E₂ and T between the male and female was significantly different(P<0.05), respectively. Results suggested that the relative content of E₂ and T may result in the growth difference between the male and female Nile tilapia.

Key words: Oreochromis niloticus, female and male, steroid hormones

中图分类号:

S917.4

岳蒙蒙, 赵金良, 唐首杰, 王燕, 赵永华. 性成熟前期尼罗罗非鱼雌雄性别生长差异与性类固醇激素水平比较[J]. 浙江农业学报, 2016, 28(10): 1678-1686.

YUE Meng-meng, ZHAO Jin-liang, TANG Shou-jie, WANG Yan, ZHAO Yong-hua. Study on growth difference and steroid hormones between female and male Nile tilapia Oreochromis niloticus before sexual maturity[J]. , 2016, 28(10): 1678-1686.

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性成熟前期尼罗罗非鱼雌雄性别生长差异与性类固醇激素水平比较

Study on growth difference and steroid hormones between female and male Nile tilapia Oreochromis niloticus before sexual maturity

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