浙江农业学报 ›› 2016, Vol. 28 ›› Issue (12): 1992-1999.DOI: 10.3969/j.issn.1004-1524.2016.12.05

• 动物科学 • 上一篇    下一篇

四川省腹泻病猪的猪萨佩罗病毒检测及1B基因序列比较分析

李雨濛a, 郭博a, 徐逸飞a, 陈瑛琪a, 蔡瑶a, 李小璟a, 龚双燕a, 徐志文a,b, 朱玲a,b,*   

  1. 四川农业大学 a.动物生物技术中心; b.动物疫病与人类健康四川省重点实验室,四川 成都 611130
  • 收稿日期:2016-05-13 出版日期:2016-12-15 发布日期:2017-01-05
  • 通讯作者: 朱玲,E-mail: abtczl72@126.com
  • 作者简介:李雨濛(1993—),男,四川成都人,硕士研究生,研究内容项缺失 E-mail: 664294092@qq.com
  • 基金资助:
    四川省科技支撑计划(2014NZ0043); 长江学者与创新团队发展计划(IRT13083); 四川省农作物及畜禽育种攻关项目; “十二五”农村领域国家科技计划课题(2015BAD12B04-2.3)

Epidemiological survey of porcine sapelovirus and genetic variation analysis of 1B gene in Sichuan Province

LI Yu-menga, GUO Boa, XU Yi-Feia, CHEN Ying-qia, CAI Yaoa, LI Xiao-Jinga, GONG Shuang-yana, XU Zhi-wena,b, ZHU Linga,b,*   

  1. a. Animal Biotechnology Center; b. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China
  • Received:2016-05-13 Online:2016-12-15 Published:2017-01-05

摘要: 为了解猪萨佩罗病毒(porcine sapelovirus, PSV)的流行及变异情况,采集2015-2016年四川地区12个县市34个猪场共计428份腹泻病料,采用QRT-PCR方法对PSV进行检测。结果显示:在428份病料中,共有114份为PSV阳性,阳性率为26.6%;所检测的34个猪场中,共有20个猪场显示PSV阳性,猪场阳性率为58.8%,表明PSV在四川地区普遍存在。此外,对来自不同县市共14份PSV阳性病料1B全基因进行PCR扩增,通过分子克隆,测序,再利用序列分析软件对四川部分地区猪萨佩罗病毒1B基因进行序列分析。结果表明,14条猪萨佩罗病毒1B基因核苷酸序列及推导出的氨基酸序列相似性分别为90.6%~100%和97.1%~100%。遗传进化分析显示,该研究获得的四川株与已知中国株分属于一个大的分支,说明我国猪萨佩罗病毒1B基因序列较为保守,没有发生大的基因变异。所有中国分离株与韩国株、德国株、英国株处在不同分支,又说明我国PSV的流行毒株可能存在独立进化。

关键词: 猪萨佩罗病毒, 流行病学调查, 1B基因, 遗传进化分析

Abstract: To investigate the prevalence of porcine sapelovirus (PSV), a total of 428 fecal samples from pigs with diarrhea were collected from 34 pig farms during 2015 to 2016 in Sichuan Province, and PSV in these samples was detected by using qRT-PCR. The results revealed that 114 (26.6%) samples and 20 (58.8%) farms were positive for PSV, which indicated that PSV infections in pig herds were endemic in Sichuan province. The 14 novel PSV 1B nucleotide sequences were obtained by RT-PCR and confirmed by sequencing (GenBank accession No.: KU323644-KU323657). Nucleotide and the deduced amino acid sequence homologies were 90.6%-100% and 97.1%-100%, respectively. Phylogenetic analysis indicated that the novel Sichuan strains belong to a group with the strains from other provinces of China. There was no major gene mutation, which showed that the gene of PSV was highly conserved in China. The novel Sichuan strains,Korea strains,Germany strains and Britain strains were in different branches, which could indicate that the prevalence of PSV in our country might be independent evolution.

Key words: porcine sapelovirus, epidemiological survey, 1B gene, genetic variation

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