大熊猫卵巢OB-Rb、NPY的蛋白表达与分布研究

doi:10.3969/j.issn.1004-1524.2016.12.06

浙江农业学报 ›› 2016, Vol. 28 ›› Issue (12): 2000-2006.DOI: 10.3969/j.issn.1004-1524.2016.12.06

大熊猫卵巢OB-Rb、NPY的蛋白表达与分布研究

罗启慧¹, 王承东², 李德生², 邓林华², 李才武², 陈正礼^1,*, 黄超¹

1.四川农业大学动物医学院, 实验动物疾病模型研究室, 四川成都 611130;
2.中国保护大熊猫研究中心, 濒危动物遗传与育种四川省重点实验室, 四川雅安 625014

收稿日期:2016-04-29 出版日期:2016-12-15 发布日期:2017-01-05
通讯作者: 陈正礼,E-mail: chzhli75@163.com
作者简介:罗启慧(1980—),女,重庆荣昌人,副教授,从事实验动物疾病模型研究。E-mail: lqhbiology@163.com
基金资助:
中国保护大熊猫研究中心项目; 国家科技支撑计划课题(2014BAI03B01); 国家重大科学仪器设备开发专项(2013YQ49085906)

Expression and distribution of OB-Rb and NPY in ovary of giant panda

LUO Qi-hui¹, WANG Cheng-dong², LI De-sheng², DENG Lin-hua², LI Cai-wu², CHEN Zheng-li^1,*, HUANG Chao¹

1. Laboratory of Animal Disease Model, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. Key Laboratory for Reproduction and Conservation Genetics of Endangered Wildlife of Sichuan Province, China Conservation and Research Center for the Giant Panda, Ya'an 625014, China;

Received:2016-04-29 Online:2016-12-15 Published:2017-01-05

摘要/Abstract

摘要： 为了探究瘦素长型受体(OB-Rb)与神经肽Y(neuropeptide Y,NPY)在大熊猫生殖调控中的作用,运用HE法、免疫组化SABC法观察了5例大熊猫卵巢组织学结构及OB-Rb、NPY在卵巢的表达与分布。结果显示:大熊猫卵巢各级卵泡数量较少,尤以中晚期生长卵泡更甚,原始卵泡和闭锁卵泡相对多见。OB-Rb和NPY在卵巢上均有表达,其中OB-Rb主要分布在颗粒细胞、卵泡膜细胞及黄体的粒黄体细胞;NPY阳性纤维和产物不均匀分布于卵巢各个部位,呈串珠状或点状。NPY阳性神经纤维主要位于血管周围、卵泡、黄体以及颗粒细胞中。大熊猫卵巢卵泡数量少的特征可能与其生殖能力低下有关;OB-Rb和NPY参与了卵巢卵泡发育的调控。

关键词: NPY, OB-Rb, 免疫组化, 卵巢, 大熊猫

Abstract: In order to explore the effect of long-form leptin receptor (OB-Rb) and neuropeptide Y (NPY) on reproductive regulation of giant panda, the ovarian histology structure, expression of OB-Rb and NPY in ovaries of 5 cases of giant panda were studied by HE staining and immunohistochemical SABC. Results showed that the numbers of ovarian follicles in different stages were small, especially the middle or late follicle, while the numbers of primitive follicles and atresia follicles were relatively greater. OB-Rb and NPY were expressed in ovaries. The OB-Rb positive products were distributed in granulosa cells, theca cells and granular lutein cells of corpus luteum. The NPY positive fiber and products were unevenly distributed in ovarian parts, which formed as beads or dots. The NPY positive nerve mainly distributed around blood vessels, in follicular, corpus luteum and granulosa cells. Results showed that small number of follicle was the features of giant panda's ovarian, which may be closely related with its low reproductive ability, and OB-Rb and NPY participated in the regulation of ovarian follicle development.

Key words: NPY, OB-Rb, immunohistochemical, ovary, giant panda

中图分类号:

S864.1

罗启慧, 王承东, 李德生, 邓林华, 李才武, 陈正礼, 黄超. 大熊猫卵巢OB-Rb、NPY的蛋白表达与分布研究[J]. 浙江农业学报, 2016, 28(12): 2000-2006.

LUO Qi-hui, WANG Cheng-dong, LI De-sheng, DENG Lin-hua, LI Cai-wu, CHEN Zheng-li, HUANG Chao. Expression and distribution of OB-Rb and NPY in ovary of giant panda[J]. , 2016, 28(12): 2000-2006.

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大熊猫卵巢OB-Rb、NPY的蛋白表达与分布研究

Expression and distribution of OB-Rb and NPY in ovary of giant panda

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