动物园圈养大熊猫毕氏肠微孢子虫分子流行病学调查

doi:10.3969/j.issn.1004-1524.2018.07.05

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (7): 1132-1136.DOI: 10.3969/j.issn.1004-1524.2018.07.05

动物园圈养大熊猫毕氏肠微孢子虫分子流行病学调查

曹钰莹¹, 李威¹, 钟志军¹, 肖洁¹, 弓超¹, 王承东², 李才武², 杨海迪², 黄祥明³, 吴孔菊^{3, *}, 彭广能^{1, *}

1.四川农业大学动物医学院动物疫病与人类健康四川省重点实验室,四川成都 611130;
2.中国大熊猫保护研究中心卧龙基地,四川阿坝 624000;
3.成都大熊猫繁育研究基地,四川成都 610081

收稿日期:2017-11-02 出版日期:2018-07-20 发布日期:2018-08-02
通讯作者: 彭广能,E-mail: pgn.sicau@163.com;吴孔菊,E-mail: 646401864@qq.com
作者简介:曹钰莹(1997—),女,四川大邑人,本科生。E-mail: 13558612648@163.com
基金资助:
成都大熊猫繁育研究基金会(CPF2015-07,CPF2015-09,CPF2014-12)

Molecular characterization of Enterocytozoon bieneusi in captive giant panda, China

CAO Yuying¹, LI Wei¹, ZHONG Zhijun¹, XIAO Jie¹, GONG Chao¹, WANG Chengdong², LI Caiwu², YANG Haidi², HUANG Xiangming³, WU Kongju^{3, *}, PENG Guangneng^{1, *}

1. Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. Wolong Base of China Conservation and Research Center for Giant Panda, Aba 624000, China;
3. Chengdu Research Base of Giant Panda Breeding, Chengdu 610081, China

Received:2017-11-02 Online:2018-07-20 Published:2018-08-02

摘要/Abstract

摘要： 为了解各地动物园圈养大熊猫的毕氏肠微孢子虫感染情况及其人畜共患风险,采用基于ITS基因的巢氏PCR方法,对江苏、福建、浙江等10个省(市、自治区)的动物园圈养大熊猫毕氏肠微孢子虫的感染情况及基因分型进行调查和检测。在31份大熊猫粪便样品中,共检测到毕氏肠微孢子虫阳性14份,总感染率45.2%,检测出SC02(13/14)和Peru6(1/14)两种毕氏肠微孢子虫基因型。种系发育分析表明,SC02和Peru6都属于具有人畜共患潜能的group 1b。本实验首次发现SC02基因型毕氏肠微孢子虫感染大熊猫,表明大熊猫可能作为毕氏肠微孢子虫的储存宿主引起人微孢子虫病,拓宽了基因型SC02的毕氏肠微孢子虫宿主范围。

关键词: 毕氏肠微孢子虫, 大熊猫, 巢氏PCR, ITS基因型, 动物园

Abstract: In order to reveal the prevalence of Enterocytozoon bieneusi in captive giant panda and assess its zoonotic potential, we surveyed 31 fecal samples from captive giant panda based on nested PCR amplification of ITS gene of E. bieneusi. A total of 14 samples were positive for E. bieneusi, with an infection rate of 45.2%. Two genotypes were identified as SC02(13/14)and Peru6(1/14). Phylogenetic analysis revealed that these genotypes belonged to group 1b which had zoonotic potential. Giant panda could spread E. bieneusi to humans and lead to human microsporidiosis. The first report of SC02 in giant panda broadened the understanding of E. bieneusi of hosts.

Key words: Enterocytozoon bieneusi, giant panda, nested PCR, ITS gene, zoo

中图分类号:

S855.9

曹钰莹, 李威, 钟志军, 肖洁, 弓超, 王承东, 李才武, 杨海迪, 黄祥明, 吴孔菊, 彭广能. 动物园圈养大熊猫毕氏肠微孢子虫分子流行病学调查[J]. 浙江农业学报, 2018, 30(7): 1132-1136.

CAO Yuying, LI Wei, ZHONG Zhijun, XIAO Jie, GONG Chao, WANG Chengdong, LI Caiwu, YANG Haidi, HUANG Xiangming, WU Kongju, PENG Guangneng. Molecular characterization of Enterocytozoon bieneusi in captive giant panda, China[J]. , 2018, 30(7): 1132-1136.

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动物园圈养大熊猫毕氏肠微孢子虫分子流行病学调查

Molecular characterization of Enterocytozoon bieneusi in captive giant panda, China

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