奶牛静脉血和脐静脉血脂联素、瘦素及IGF-1表达水平与犊牛初生体质量相关性研究

doi:10.3969/j.issn.1004-1524.2017.01.06

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (1): 37-43.DOI: 10.3969/j.issn.1004-1524.2017.01.06

奶牛静脉血和脐静脉血脂联素、瘦素及IGF-1表达水平与犊牛初生体质量相关性研究

沈留红, 江涛, 肖劲邦, 姜思汛, 巫晓峰, 曹随忠, 余树民, 邓俊良, 彭广能, 左之才

四川农业大学动物医学院动物疫病与人类健康四川省重点实验室/奶牛疾病研究中心, 四川成都 611130

收稿日期:2016-05-11 出版日期:2017-01-15 发布日期:2017-02-23
作者简介:沈留红(1979—),男,江苏如皋人,博士,副教授,从事反刍动物疾病及繁殖新技术研究。 E-mail:shenlh@sicau.edu.cn
基金资助:
四川省教育厅重点项目(15ZA0024); 四川省科技厅科技支撑项目(2011NZ0060,2013NZ0032); 四川农业大学双支计划

Correlation between calf birth weights and expression levels of adiponectin, leptin, IGF-1 in cow's venous blood and cord venous blood

SHEN Liuhong, JIANG Tao, XIAO Jinbang, JIANG Sixun, WU Xiaofeng, CAO Suizhong, YU Shumin, DENG Junliang, PENG Guangneng, ZUO Zhicai

Key Laboratory of Animal Disease and Human Health of Sichuan Province, Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China

Received:2016-05-11 Online:2017-01-15 Published:2017-02-23

摘要/Abstract

摘要： 为探究奶牛静脉血及脐静脉血中脂联素(adiponectin)、瘦素(leptin)、胰岛素样生长因子1(IGF-1)与犊牛初生体质量间的相关性。选取规模化养殖场54头正常分娩奶牛,按犊牛初生体质量划分为A(≤40 kg),B(40~45 kg),C(≥45 kg)3组,采集分娩奶牛静脉血及脐静脉血,用ELISA方法检测静脉血中脂联素、瘦素和IGF-1的表达水平,分析其间的相关性及与犊牛初生体质量的相关性。结果表明,奶牛静脉血脂联素、瘦素和IGF-1表达水平两两之间均呈极显著正相关(P<0.01),但奶牛静脉血脂联素、瘦素和IGF-1表达水平与犊牛初生体质量相关性均不显著(P>0.05);脐静脉血脂联素、瘦素和IGF-1表达水平两两之间均呈极显著正相关(P<0.01),脐静脉血脂联素、瘦素和IGF-1表达水平均随犊牛初生体质量增加而逐渐升高,且分别与犊牛初生体质量呈极显著正相关(P<0.01);奶牛静脉血脂联素、瘦素和IGF-1表达水平均极显著高于脐静脉血(P<0.01),而奶牛静脉血脂联素、瘦素和IGF-1的表达水平与脐静脉血中表达水平相关性均不显著(P>0.05)。据此得出,奶牛静脉血和脐静脉血中均有脂联素、瘦素和IGF-1表达,奶牛静脉血中及脐静脉血中脂联素、瘦素和IGF-1均各自相互协调发挥作用;奶牛静脉血脂联素、瘦素和IGF-1均对犊牛初生体质量影响不显著,但脐静脉血脂联素、瘦素和IGF-1均对犊牛初生体质量影响极显著,该结果可为进一步研究脂联素、瘦素和IGF-1对犊牛初生体质量的影响机制奠定基础。

关键词: 脂联素, 瘦素, 初生体质量, 奶牛静脉血, 脐静脉血

Abstract: In order to explore the correlation between calf birth weights and expression level of adiponectin, leptin, IGF-1 in cow's venous and cord venous blood. Fifty-four healthy Chinese Holstein cows that experienced normal natural birth were used, and were divided into 3 groups based on calf birth weights (A, ≤40 kg; B, 40~45 kg; C, ≥45 kg). The cow's venous blood and cord venous blood was collected. ELISA was used to evaluate the expression levels of adiponectin, leptin and IGF-1 to analyze the correlation among them as well as correlation with calf birth weights. It was shown that there were significant (P<0.01) correlations among adiponectin, leptin and IGF-1 in cow's venous blood, while these indexes showed no significant (P>0.05) correlations with calf birth weights. In cow's cord venous blood, adiponectin, leptin, and IGF-1 were significantly (P<0.01) correlated, and adiponectin, leptin, and IGF-1 in cord venous blood as whole were positively correlated with calf birth weights, and was significantly (P<0.01) correlated with calf birth weights individually. Adiponectin, leptin, and IGF-1 expression levels in venous blood were significantly (P<0.01) higher than those in cord venous blood, and were not significantly (P>0.05) correlated with those in cord venous blood. The results showed that there were adiponectin, leptin, and IGF-1 in venous blood and cord venous blood. Adiponectin, leptin, and IGF-1 in venous and cord venous blood were potentially inter-regulated with each other. Adiponectin, leptin, and IGF-1 expression levels in venous blood were not significantly (P<0.05) correlated with calf birth weights, while adiponectin, leptin, and IGF-1 expression levels in cord venous blood were significantly (P<0.01) correlated with calf birth weights, respectively.

Key words: adiponectin, leptin, birth weights, venous blood, cord venous blood

中图分类号:

S852.5

沈留红, 江涛, 肖劲邦, 姜思汛, 巫晓峰, 曹随忠, 余树民, 邓俊良, 彭广能, 左之才. 奶牛静脉血和脐静脉血脂联素、瘦素及IGF-1表达水平与犊牛初生体质量相关性研究[J]. 浙江农业学报, 2017, 29(1): 37-43.

SHEN Liuhong, JIANG Tao, XIAO Jinbang, JIANG Sixun, WU Xiaofeng, CAO Suizhong, YU Shumin, DENG Junliang, PENG Guangneng, ZUO Zhicai. Correlation between calf birth weights and expression levels of adiponectin, leptin, IGF-1 in cow's venous blood and cord venous blood[J]. , 2017, 29(1): 37-43.

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奶牛静脉血和脐静脉血脂联素、瘦素及IGF-1表达水平与犊牛初生体质量相关性研究

Correlation between calf birth weights and expression levels of adiponectin, leptin, IGF-1 in cow's venous blood and cord venous blood

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