中国荷斯坦奶牛Nramp1基因多态性与泌乳性状及产奶量的关联性分析

doi:10.3969/j.issn.1004-1524.2017.03.06

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (3): 389-394.DOI: 10.3969/j.issn.1004-1524.2017.03.06

中国荷斯坦奶牛Nramp1基因多态性与泌乳性状及产奶量的关联性分析

张丽, 刘丽霞, 陈红, 李强子, 刘吴鑫

西北民族大学生命科学与工程学院,甘肃兰州730030

收稿日期:2016-09-16 出版日期:2017-03-20 发布日期:2017-03-31
作者简介:张丽(1980—),女,宁夏石嘴山人,博士,副教授,主要从事奶牛分子育种研究。E-mail:zhangli2008@aliyun.com
基金资助:
西北民族大学引进人才科研项目(xbmuyjrc201316); 西北民族大学基本科研业务费专项资金资助项目(31920140077)

Genetic polymorphism Nramp1 gene and its associations with milk traits and milk yield in Chinese Holstein cattle

ZHANG Li, LIU Lixia, CHEN Hong, LI Qiangzi, LIU Wuxin

College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730030, China

Received:2016-09-16 Online:2017-03-20 Published:2017-03-31

摘要/Abstract

摘要： 为寻找与乳房炎相关的分子标记,为荷斯坦牛的抗病育种提供理论基础,试验采用PCR-SSCP技术和测序的方法对宁夏农垦303头中国荷斯坦奶牛的Nramp1基因进行了遗传多态性分析,利用一般线性模型分析Nramp1基因c.200C>G突变对泌乳性状及产奶量影响。结果显示,Nramp1基因c.200C>G突变与乳脂率、日产奶量和体细胞评分值存在显著(P<0.05)或极显著(P<0.01)关联。因此,可以把该位点作为中国荷斯坦奶牛体细胞评分、测定日产奶量和日乳脂率的遗传标记进行进一步研究。

关键词: 荷斯坦牛, Nramp1基因, 遗传多态性, 泌乳性状, 产奶量

Abstract: To search the molecular markers that are associated with mastitis and to provide a theoretical basis for the Holstein cattle breeding and disease resistance, the polymoprhisms of Nramp1 gene in 303 Holstein cattle in Ningxia were detected by the PCR-SSCP technique and the effect of Nramp1 gene c.200C>G locus mutation on milk traits and milk yield was analyzed using a general linear model. The results showed that there was a significant relationship between Nramp1 gene c.200C>G locus mutation and milk fat, milk yield and somatic cell score at the probability level of 0.05 or 0.01. Therefore we can put the site as cattle somatic cell score, daily milk yield measurement and daily milk fat genetic markers in Chinese Holstein.

Key words: Holstein cattle, Nramp1 gene, genetic polymorphism, milk traits, milk yield

中图分类号:

S823.2

张丽, 刘丽霞, 陈红, 李强子, 刘吴鑫. 中国荷斯坦奶牛Nramp1基因多态性与泌乳性状及产奶量的关联性分析[J]. 浙江农业学报, 2017, 29(3): 389-394.

ZHANG Li, LIU Lixia, CHEN Hong, LI Qiangzi, LIU Wuxin. Genetic polymorphism Nramp1 gene and its associations with milk traits and milk yield in Chinese Holstein cattle[J]. , 2017, 29(3): 389-394.

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中国荷斯坦奶牛Nramp1基因多态性与泌乳性状及产奶量的关联性分析

Genetic polymorphism Nramp1 gene and its associations with milk traits and milk yield in Chinese Holstein cattle

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