缓释碳源与净水菌胶囊组合脱氮净水性能研究

doi:10.3969/j.issn.1004-1524.2017.04.20

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (4): 651-659.DOI: 10.3969/j.issn.1004-1524.2017.04.20

缓释碳源与净水菌胶囊组合脱氮净水性能研究

闫秋月¹, 柳永², 王新², 孙宏², 姚晓红², 吴逸飞², 汤江武^{2, *}, 葛向阳^{1, *}

1.华中农业大学农业微生物学国家重点实验室,湖北武汉 430070;
2.浙江省农业科学院植物保护与微生物研究所,浙江杭州 310021

收稿日期:2016-09-17 出版日期:2017-04-20 发布日期:2017-04-27
通讯作者: 汤江武,E-mail:tangjiangwu@sina.com;葛向阳,E-mail:gxy@mail.hzau.edu.cn
作者简介:闫秋月(1990—),女,山东聊城人,硕士研究生,研究方向为环境微生物学。E-mail:1558246742@qq.com
基金资助:
浙江省公益技术应用研究项目(2015C33046); 浙江省重大科技专项(2015C03004); 浙江省农科院创新提升工程项目; 温州市水体污染控制与治理科技创新项目(S20140021)

Research on co-denitrification performance of slow-release organic carbon source and water purification bacteria-embedded solid capsules

YAN Qiuyue¹, LIU Yong², WANG Xin², SUN Hong², YAO Xiaohong², WU Yifei², TANG Jiangwu^{2, *}, GE Xiangyang^{1, *}

1. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China;
2. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2016-09-17 Online:2017-04-20 Published:2017-04-27

摘要/Abstract

摘要： 在低碳氮比污水治理中,人工投加碳源和菌剂是必要手段,为此,特开展缓释碳源与净水菌胶囊的组合净水性能研究。试验用缓释碳源为乙酸钠包埋物,采用扫描电子显微镜观察释放前后形貌,并用高效液相色谱法进行检测。净水菌胶囊为包含施氏假单胞菌ADH1、肠杆菌AOZ1与枯草芽孢杆菌BSK9包埋物的W-capsule和B-capsule。试验水源为河道污水,在水桶中间歇曝气条件下开展试验。以无投加污水为对照,比较直投速溶碳源、直投未包埋菌剂、单独投加缓释碳源、单独投加净水菌胶囊以及组合投加缓释碳源和净水菌胶囊各试验组水体氨氮、总氮、COD等指标变化。结果表明,缓释碳源释放乙酸钠后内部结构变疏松,形成大量刨花样卷曲片层结构。净水试验中:投加缓释碳源组总氮的去除率达到60.2%,且试验过程中COD始终低于20 mg·L^-1;投加固定化菌剂W-capsule组总氮去除率达39.1%;组合投加缓释碳源和净水菌胶囊组总氮去除率可达85.2%。可见,缓释碳源与净水菌胶囊组合在河道污水脱氮上表现出较高性能,应用潜力巨大。

关键词: 乙酸钠, 生物脱氮, 反硝化菌, 氨氧化菌

Abstract: For the treatment of wastewater with low carbon and high nitrogen, the indispensable and effective manner is to launch carbon and microbial inoculum. In this study, investigations were performed into the water purifying capacity of a novel and comprehensive management strategy, which integrated sustained-release carbon source and water purification bacteria-embedded solid capsules. Sodium acetate was used as the carbon source and embedded with carrier materials. Scanning electron microscopy (SEM) was carried out to analyze micro-morphological changes to the entrapments before and after release of the carbon source. The released carbon source (sodium acetate) was detected using high performance liquid chromatography (HPLC). The bacteria-embedded solid capsules were divided into W-capsule and B-capsule, which included Pseudomonas stutzeri ADH1, Enterobacter sp. AOZ1 and Bacillus subtilis BSK9. Experiments were performed on river sewage in buckets with intermittent aeration to investigate the change to some routine indexes covering ammonia nitrogen, total nitrogen (TN) and chemical oxygen demand (COD), etc. Sewage treated using instant/sustained-release carbon source and bacteria with/without embedding were tested as comparable controls. Untreated sewage was also tested as a negative control (NC). It was shown that extensive wood shaving-like structures formed inside the entrapments after the release of sodium acetate. TN remove rate of the sewage treated with sustained-release carbon source was 60.2%, and the COD kept less than 20 mg·L^-1. For the sewage treated with W-capsule and the combination of sustained-release carbon source and bacteria-embedded capsules, the TN remove rate was 39.1% and 85.2%, respectively, which indicated high performance of the novel combinatorial strategy in nitrogen removal of river sewage.

Key words: sodium acetate, biological denitrification, denitrifying bacteria, nitrifying bacteria

中图分类号:

X522

闫秋月, 柳永, 王新, 孙宏, 姚晓红, 吴逸飞, 汤江武, 葛向阳. 缓释碳源与净水菌胶囊组合脱氮净水性能研究[J]. 浙江农业学报, 2017, 29(4): 651-659.

YAN Qiuyue, LIU Yong, WANG Xin, SUN Hong, YAO Xiaohong, WU Yifei, TANG Jiangwu, GE Xiangyang. Research on co-denitrification performance of slow-release organic carbon source and water purification bacteria-embedded solid capsules[J]. , 2017, 29(4): 651-659.

参考文献

[1] 吴泽宁,田桂桂,王慧亮. 基于物质循环的河道内生态用水价值及其能值评估[J]. 南水北调与水利科技, 2016, 14(2): 6-10.
WU Z N, TIAN G G, WANG H L, et al. Value of instream ecological water use and the emerge evaluation method based on material cycle[J]. South-to-North Water Transfers and Water Science & Technology , 2016, 14(2): 6-10. (in Chinese with English abstract)
[2] SIERRA-ALVAREZ R, BERISTAIN-CARDOSO R, SALAZAR M, et al. Chemolithotrophic denitrification with elemental sulfur for groundwater treatment[J]. Water Research , 2007, 41(6):1253-1262.
[3] 赵佩文. 不同氮污染水体中微生物对环境的生态响应[D]. 武汉:华中农业大学, 2016.
ZHAO P W. The ecological respond of microorganisms to environment in different nitrogen polluted water[D]. Wuhan: Huazhong Agricultural University, 2016. (in Chinese with English abstract)
[4] 王淑莹,殷芳芳,侯红勋,等. 以甲醇作为外碳源的生物反硝化[J].北京工业大学学报, 2009, 35(11): 1522-1526.
WANG S Y, YIN F F, HOU H X, et al. Biological denitrification with methanol as external carbon source[J]. Journal of Beijing University of Technology , 2009, 35(11): 1522-1526. (in Chinese with English abstract)
[5] 蒋亚萍,陈余道,刘汉乐,等. 乙醇在地下水硝酸盐原位去除中的可利用性[J]. 自然资源学报, 2011, 26(8): 1447-1452.
JING Y P, CHEN Y D, LIU H L, et al. Availability of ethanol for in-situ nitrate bioremediation in polluted groundwater[J]. Journal of Natural Resources , 2011, 26(8): 1447-1452. (in Chinese with English abstract)
[6] 李洪静, 陈银广, 顾国维. 丙酸/乙酸对低能耗生物除磷脱氮系统的影响[J]. 中国环境科学, 2008, 28(8):673-678.
LI H J, CHEN Y G, GU G W. Effect of propionic to acetic acid ratio on biological nitrogen and phosphorus removal with low energy consumption[J]. China Environmental Science , 2008, 28(8): 673-678. (in Chinese with English abstract)
[7] 王丽丽,赵林,谭欣,等. 不同碳源及其碳氮比对反硝化过程的影响[J]. 环境保护科学, 2004, 30(1): 15-18.
WANG L L,ZHAO L,TAN X,et al. Influence of different carbon source and ratio of carbon and nitrogen for water denitrification[J]. Environmental Protection Science , 2004, 30(1): 15-18. (in Chinese with English abstract)
[8] 王允,张旭,张大奕,等. 用于地下水原位生物脱氮的缓释碳源材料性能研究[J]. 环境科学, 2008, 29(8): 2183-2188.
WANG Y, ZHANG X, ZHANG D Y, et al. Performance of slow-release organic carbon-source (SOC) materials for in-situ biological denitrification in groundwater[J]. Environmental Science , 2008, 29(8): 2183-2188. (in Chinese with English abstract)
[9] 王润众, 郝瑞霞, 赵文莉. 新型缓释碳源的制备及其性能[J]. 环境工程学报, 2016, 10(1):81-87.
WANG R Z, HAO R X, ZHAO W L. Preparation and properties of new slow-release organic carbon source[J]. Chinese Journal of Environmental Engineering , 2016, 10(1): 81-87. (in Chinese with English abstract)
[10] MICHAL V, AHARON A, MIM S. Denitrification of groundwater using cotton as energy source[J]. Water Science and Technology , 1996, 34(1): 379-385.
[11] 李斌,郝瑞霞. 固体纤维素类废物作为反硝化碳源滤料的比选[J]. 环境科学, 2013, 34(4): 1428-1434.
LI B, HAO R X. Comparison and optimization of cellulose carbon source for denitrification filter[J]. Environmental Science , 2013, 34(4): 1428-1434. (in Chinese with English abstract)
[12] 赵文莉,郝瑞霞,李斌,等. 预处理方法对玉米芯作为反硝化固体碳源的影响[J]. 环境科学, 2014, 35(3): 987-994.
ZHAO W L, HAO R X, LI B, et al. Effects of pretreatment methods on corncob as carbon source for denitrification[J]. Environmental Science , 2014, 35(3): 987-994. (in Chinese with English abstract)
[13] SOARES M I M, ABELIOVICH A. Wheat straw as substrate for water denitrification[J]. Water Research , 1998, 32(12):3790-3794.
[14] ASLAN S, TÜRKMAN A. Biological denitrification of drinking water using various natural organic solid substrates[J]. Water Science & Technology , 2003, 48(11/12):489-495.
[15] 赵凯,黄盼宁,鲍捷. 固体缓释碳源辅助低碳氮比污水脱氮技术研究[J]. 辽宁化工, 2015, 44(8): 1037-1039.
ZHAO K, HUANG P N, BAO J. Research progress in low C/N wastewater denitrification by using solid sustained release carbon materials[J]. Liaoning Chemical Industry , 2015, 44(8): 1037-1039. (in Chinese with English abstract)
[16] 张大奕,李广贺,王允,等. 缓释碳源材料释碳与脱氮性能[J]. 清华大学学报(自然科学版), 2009, 49(9): 75-79.
ZHANG D Y, LI G H, WANG Y, et al. Slow-release organic carbon-source materials for groundwater in-situ denitrification[J]. Journal of Tsinghua University ( Science and Technology ), 2009, 49(9): 75-79. (in Chinese with English abstract)
[17] 范振兴,王建龙. 利用聚乳酸作为反硝化固体碳源的研究[J]. 环境科学, 2009, 30(8): 2315-2319.
FAN Z X, WANG J L. Denitrification using polylactic acid as solid carbon source[J]. Environmental Science , 2009, 30(8): 2315-2319. (in Chinese with English abstract)
[18] 杨帆,王鹤立. 缓释碳源复合材料的制备及其用于地下水硝酸盐污染修复的研究[J]. 水处理技术, 2013,39(4): 75-79.
YANG F, WANG H L. Preparation of slow-release carbon source composite materials and their use for groundwater nitrate pollution restoration[J]. Technology of Water Treatment , 2013, 39(4): 75-79. (in Chinese with English abstract)
[19] 唐丹琦,王娟,郑天龙,等. 聚乳酸/淀粉固体缓释碳源生物反硝化研究[J]. 环境科学, 2014, 35(6): 2436-2440.
TANG D Q, WANG J, ZHENG T L, et al. Effect of PLA/starch slow-release carbon source on biological denitrification[J]. Environmental Science , 2014, 35(6): 2436-2440. (in Chinese with English abstract)
[20] 乔培培, 陈丕茂, 秦传新,等. 利用微生物净水研究进展[J]. 广东农业科学, 2014, 41(1):149-154.
QIAO P P, CHEN P M, QIN C X, et al. Research progress on water purification by microorganism[J]. Guangdong Agricultural Sciences , 2014, 41(1): 149-154. (in Chinese with English abstract)
[21] 李尧, 张振家, 方海军. 包埋固定化微生物工艺技术处理高氨氮化工废水[J]. 净水技术, 2012, 31(3):32-35.
LI Y, ZHANG Z J, FANG H J. Treatment of chemical industrial wastewater for high ammonia-nitrogen with embedded immobilized microorganisms technological processes[J]. Water Purification Technology , 2012, 31(3): 32-35. (in Chinese with English abstract)
[22] LIN M, LIU Y H, CHEN W W, et al. Use of bacteria-immobilized cotton fibers to absorb and degrade crude oil[J]. International Biodeterioration & Biodegradation , 2014, 88: 8-12.
[23] 王青松,柳永,王新,等. 基于质构量化分析的净水菌胶囊制备及其性能研究[J]. 浙江大学学报(农业与生命科学版), 2015, 41(6): 712-722.
WANG Q S, LIU Y, WANG X, et al. Preparation and performance study of water purification bacteria-embedded solid capsules based on texture profile analysis[J]. Journal of Zhejiang University ( Agriculture & Life Sciences ), 2015, 41(6): 712-722. (in Chinese with English abstract)
[24] 周莉. 用于污染河道修复的生物脱氮菌株的筛选及脱氮条件的研究[D]. 武汉:华中农业大学, 2011.
ZHOU L. Screening microorganisms for the bioremediation of polluted river and research of its denitrification conditions[D]. Wuhan: Huazhong Agricultural University, 2011. (in Chinese with English abstract)
[25] 胡金星. 好氧反硝化细菌的分离鉴定及反硝化特性的研究[D]. 武汉:华中农业大学, 2012.
HU J X. Isolation and identification of aerobic denitrifiers and study on denitrification characteristics[D]. Wuhan: Huazhong Agricultural University, 2012. (in Chinese with English abstract)
[26] 王青松. 柠檬酸缓释膜与质构量化净水菌胶囊的制备及其性能研究[D]. 武汉:华中农业大学, 2015.
WANG Q S. Preparation and properties of the citric acid sustained-release membrane and water purification bacteria-embedding capsules based on texture profile analysis[D]. Wuhan: Huazhong Agricultural University, 2015. (in Chinese with English abstract)

缓释碳源与净水菌胶囊组合脱氮净水性能研究

Research on co-denitrification performance of slow-release organic carbon source and water purification bacteria-embedded solid capsules

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

编辑推荐

Metrics

本文评价

[1]	潘晓艺, 蔺凌云, 尹文林, 徐洋, 姚嘉赟, 袁雪梅, 郑群艳, 沈锦玉. 氨氧化菌的分离鉴定及其在罗氏沼虾育苗废水中的应用[J]. 浙江农业学报, 2017, 29(6): 894-902.
[2]	张智超, 孙宏, 吴逸飞, 王新, 姚晓红, 柳永, 汤江武, 葛向阳. 基于PCR-DGGE技术辅助筛选氨氮降解菌株[J]. 浙江农业学报, 2017, 29(2): 286-291.