复方中药制剂对SD大鼠血清抗氧化功能的影响

doi:10.3969/j.issn.1004-1524.2017.05.08

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (5): 737-743.DOI: 10.3969/j.issn.1004-1524.2017.05.08

复方中药制剂对SD大鼠血清抗氧化功能的影响

周金星^{1, 2}, 靳二辉¹, 刘文举², 张超超¹, 王思飞¹, 金光明^{1, *}

1.安徽科技学院动物科学学院,安徽凤阳 233100；
2.安徽科技学院细胞与分子生物学研究中心,安徽凤阳 233100

收稿日期:2016-12-13 出版日期:2017-05-20 发布日期:2017-06-06
通讯作者: 金光明,E-mail: jingm@ahstu.edu.cn
作者简介:周金星(1976-),男,安徽五河人,博士,副教授,主要从事动物组织病理与中草药药理学研究。E-mail: zhoujx@ahstu.edu.cn
基金资助:
安徽省农业科技成果转化项目(1504032001); 安徽省科技攻关项目(1301042102); 安徽省高等学校自然科学研究重点项目(KJ2016A823); 安徽省第七批“115”产业创新团队项目(皖人才[2014]4号); 安徽科技学院重点建设学科项目(AKZDXK2015A04); 北京农学院兽医学(中兽医)北京市重点实验室开放课题(kf2016035)

Effect of compound Chinese medicine preparation on the antioxidant function in serum of SD rats

ZHOU Jinxing^{1, 2}, JIN Erhui¹, LIU Wenju², ZHANG Chaochao¹, WANG Sifei¹, JIN Guangming^{1, *}

1. College of Animal Science, University of Science and Technology of Anhui, Fengyang 233100, China;
2. Cell and Molecular Biology Research Center, University of Science and Technology of Anhui, Fengyang 233100, China

Received:2016-12-13 Online:2017-05-20 Published:2017-06-06

摘要/Abstract

摘要： 由黄芪、板蓝根、炒白术、茯苓、陈皮、山楂、甘草和山药等中药制成的复方中药制剂,研究其对大鼠血清总抗氧化能力(T-AOC)、超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量的影响。选择150只断奶SD大鼠(SPF级)分为对照组、1.5 mL·kg^-1组、3.0 mL·kg^-1组和6.0 mL·kg^-1组,对照组有15只大鼠,其余试验组各有45只大鼠。试验期间3个试验组大鼠按每千克体质量每天分别灌胃1.5、3.0和6.0 mL复方中药制剂(含生药1 g·mL^-1),对照组灌胃3.0 mL蒸馏水。于试验开始后10、30、60 d,每组分别取5只大鼠禁食禁水10 h后称重、心脏采血,分离血清,测定样本血清的T-AOC、T-SOD、GSH-Px活性和MDA含量。结果表明,3.0 mL·kg^-1组和6.0 mL·kg^-1组在灌胃后10 、30 和60 d,其血清T-AOC活性与同日期对照组比较有显著(P<0.05)或极显著(P<0.01)增加;而血清中MDA含量显著(P<0.05)或极显著(P<0.01)减少。3.0 mL·kg^-1组在灌胃30 和60 d,血清T-SOD活性与同日龄对照组比较显著增加(P<0.05);6.0 mL·kg^-1组在灌胃30 和60 d,血清GSH-Px活性与同日龄对照组比较显著增加(P<0.05)。说明口服以黄芪、板蓝根、炒白术等药材组成的复方中药制剂可显著提高SD大鼠的抗氧化功能,其对大鼠的最适灌胃剂量为3.0 mL·kg^-1。

关键词: 复方中药制剂, SD大鼠, 总抗氧化能力, 超氧化物歧化酶, 谷胱甘肽过氧化物酶, 丙二醛

Abstract: To study the effects of compound traditional Chinese medicine preparation, which consists of Radix astragali, Radix isatidis, Fried atractylodes, Poria cocos, orange peel, hawthorn, liquorice and yam on the T-AOC, T-SOD, GSH-Px activity and MDA content in serum of SD rats. 150 SD rats (SPF animal) were randomly divided into control group, 1.5, 3.0 and 6.0 mL·kg^-1 treatment group with 15, 45, 45, 45 SD rats, respectively. The rats of different treatment groups were administered using gavage methods according to dosage 3.0 mL·kg^-1 distilled water for control group, and 1.5, 3.0 and 6.0 mL·kg^-1 compound traditional Chinese medicine preparation (containing raw herbs 1 g·mL^-1) for treatment groups, respectively. Rats were regularly weighed and given medicine or distilled water by gavage every day. On the 10^th, 30^th and 60^th day of the experiment, 5 rats fasted and banned water for 10 h were selected in every experiment group to be weighed and picked blood from the heart, for isolating serum sample to measure T-AOC, T-SOD, GSH-Px activities and MDA content. The results showed that on the experimental 10^th, 30^th and 60^th day, the T-AOC activity in 3.0 mL·kg^-1 and 6.0 mL·kg^-1 group significantly (P<0.05) or extremely significantly increased (P<0.01), and the MDA contents in 3.0 mL·kg^-1 and 6.0 mL·kg^-1 group significantly (P<0.05) or extremely significantly decreased (P<0.01), compared with the control group on the same day. On the 30^th and 60^th day, the T-SOD activity in 3.0 mL·kg^-1 group significantly increased (P<0.05), and the GSH-Px activity in 6.0 mL·kg^-1 group significantly increased (P<0.05), compared with the control group. Compound traditional Chinese medicine preparation could significantly improve the SD rats' antioxidant function. The optimum dosage of compound traditional Chinese medicine preparation to rats was 3.0 mL·kg^-1 by gavage methods.

Key words: compound traditional Chinese medicine preparation, SD rat, T-AOC, T-SOD, GSH-Px, MDA

中图分类号:

S853.1

周金星, 靳二辉, 刘文举, 张超超, 王思飞, 金光明. 复方中药制剂对SD大鼠血清抗氧化功能的影响[J]. 浙江农业学报, 2017, 29(5): 737-743.

ZHOU Jinxing, JIN Erhui, LIU Wenju, ZHANG Chaochao, WANG Sifei, JIN Guangming. Effect of compound Chinese medicine preparation on the antioxidant function in serum of SD rats[J]. , 2017, 29(5): 737-743.

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复方中药制剂对SD大鼠血清抗氧化功能的影响

Effect of compound Chinese medicine preparation on the antioxidant function in serum of SD rats

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