生防枯草芽孢杆菌210发酵工艺优化

doi:10.3969/j.issn.1004-1524.2017.05.16

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (5): 799-805.DOI: 10.3969/j.issn.1004-1524.2017.05.16

生防枯草芽孢杆菌210发酵工艺优化

邹高溪, 赵春田, 裘娟萍^*

浙江工业大学生物工程学院,浙江杭州 310014

收稿日期:2016-12-15 出版日期:2017-05-20 发布日期:2017-06-06
通讯作者: 裘娟萍,E-mall: qiujping@zjut.edu.cn
作者简介:邹高溪(1991-),男,湖南永州人,硕士研究生,研究方向为应用微生物学。E-mall: 1575392595@qq.com
基金资助:
浙江省重中之重学科开放研究基金(20150109)

Optimization of fermentation technology of biocontrol bacterium Bacillus subtilis 210 by response surface analysis

ZOU Gaoxi, ZHAO Chuntian, QIU Juanping^*

College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China

Received:2016-12-15 Online:2017-05-20 Published:2017-06-06

摘要/Abstract

摘要： 采用响应面法,优化抗真菌的枯草芽孢杆菌210的发酵工艺,提高芽孢产量。结果显示,影响芽孢产量最主要的3个因素为玉米粉、蛋白胨和KH₂PO₄用量,最佳发酵培养基配方为:玉米粉10.75 g·L^-1、黄豆粉9.00 g·L^-1、蛋白胨6.97 g·L^-1、KH₂PO₄ 0.66 g·L^-1、NaCl 4.00 g·L^-1、MgSO₄·7H₂O 0.60 g·L^-1、CaCl₂ 3.00 g·L^-1、MnSO₄·H₂O 0.60 g·L^-1。基于优化配方通过单因素优化试验,确定最佳发酵条件为:培养温度31 ℃、装液量30 mL·250 mL^-1、接种量4%。枯草芽孢杆菌210在上述优化后的条件下培养,芽孢产量达到1.64×10¹⁰ cfu·mL^-1,较优化前提高6.5倍。

关键词: 枯草芽孢杆菌, 发酵工艺, 响应面法

Abstract: In order to increase the spore yield, the fermentation technology of Bacillus subtilis 210 with antifungal activity was optimized. It was shown that the main factors influencing spore production were doses of corn flour, peptone and KH₂PO₄. The optimal fermentation medium was as follows: corn flour 10.75 g·L^-1, soybean meal 9.00 g·L^-1, peptone 6.97 g·L^-1, KH₂PO₄ 0.66 g·L^-1, NaCl 4.00 g·L^-1, MgSO₄·7H₂O 0.60 g·L^-1, CaCl₂ 3.00 g·L^-1 and MnSO₄·H₂O 0.60 g·L^-1. Based on the single factor optimization experiment results, the optimal culture conditions were as follows∶culture temperature 31 ℃,fluid volume 30 mL·250 mL^-1,inoculum size 4%. With the optimal fermentation condition, the spore yield of Bacillus subtilis 210 reached 1.64×10¹⁰ cfu·mL^-1, and was increased by 6.5 fold as compared with the spore yield before fermentation technology optimization.

Key words: Bacillus subtilis, fermentation technology, response surface analysis

中图分类号:

S482.7

邹高溪, 赵春田, 裘娟萍. 生防枯草芽孢杆菌210发酵工艺优化[J]. 浙江农业学报, 2017, 29(5): 799-805.

ZOU Gaoxi, ZHAO Chuntian, QIU Juanping. Optimization of fermentation technology of biocontrol bacterium Bacillus subtilis 210 by response surface analysis[J]. , 2017, 29(5): 799-805.

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生防枯草芽孢杆菌210发酵工艺优化

Optimization of fermentation technology of biocontrol bacterium Bacillus subtilis 210 by response surface analysis

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