对虾中隐蔽态T-2毒素残留对小鼠血常规和血清生化指标的影响

doi:10.3969/j.issn.1004-1524.2017.07.08

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (7): 1110-1118.DOI: 10.3969/j.issn.1004-1524.2017.07.08

对虾中隐蔽态T-2毒素残留对小鼠血常规和血清生化指标的影响

黄展锐¹, 王雅玲^{1, *}, 王小博¹, 莫冰², 邱妹¹, 孙力军¹, 陈康健³, 刘健³

1.广东海洋大学食品科技学院,广东省水产品加工与安全重点实验室,广东普通高等学校水产品深加工重点实验室,广东湛江 524088;
2.广西壮族自治区产品质量监督检验研究院,广西南宁 530071;
3.湛江恒兴水产科技有限公司,广东湛江 524088

收稿日期:2017-01-15 出版日期:2017-07-20 发布日期:2017-07-24
通讯作者: 王雅玲,E-mail:wangylchina@163.com
作者简介:黄展锐(1992—),男,广东梅州人,硕士研究生,研究方向为水产食品质量安全。E-mail:zhanrui789@126.com
基金资助:
国家自然科学基金面上项目(31171634,31371777); 广东省科技计划项目(2014B020205006)

Effect of masked T-2 toxin residues in Litopenaeus vannamei on routine blood and serum biochemical indexes of mice

HUANG Zhanrui¹, WANG Yaling^{1, *}, WANG Xiaobo¹, MO Bing², QIU Mei¹, SUN Lijun¹, CHEN Kangjian³, LIU Jian³

1. College of Food Science and Technology of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Zhanjiang 524088, China;
2. Products Quality Supervising Test Institute of Guangxi Zhuang Autonomous Region, Nanning 530071, China;
3. Zhanjiang Hengxing Aquatic Product Science and Technology Co., Ltd, Zhanjiang 524088, China

Received:2017-01-15 Online:2017-07-20 Published:2017-07-24

摘要/Abstract

摘要： T-2毒素经20 d蓄积毒性试验法染毒对虾,采用LC-MS/MS检测对虾组织中游离态T-2毒素,并将染毒的虾头和肌肉匀浆液连续灌胃小鼠 7 d,检测小鼠血常规和血清指标,分析对虾虾头和肌肉中隐蔽态T-2毒素残留对小鼠的毒性效应。结果显示,经20 d蓄积毒性试验,对虾体内没有游离态T-2存在,而且低剂量染毒对虾对小鼠没有影响,但高剂量组 (12.2 mg·kg^-1)可引起小鼠中间粒细胞增加,红细胞系统和血小板各项指标均显著下降 (P<0.05),血清生化指标差异显著 (P<0.05),且虾头对小鼠的损伤作用高于肌肉。研究表明,蓄积染毒对虾体内存在隐蔽态T-2毒素,对小鼠具有一定的免疫毒性和血液毒性,导致炎症发生、贫血以及骨髓造血功能下降,对肝功能有损伤作用,且虾头中残留的隐蔽态T-2毒素对小鼠的损伤作用高于肌肉。

关键词: 对虾, 隐蔽态T-2毒素, 血常规, 血清

Abstract: After 20 d accumulation toxicity test of T-2 toxin, the free T-2 toxin in shrimp was quantitatively detected using LC-MS/MS. The shrimp head and muscle homogenate were administered to mice for 7 days, the blood routine and serum indexes in mice were detected to study the toxicity effect of residual masked T-2 toxin in Litopenaeus vannamei head and muscle on routine blood and serology of mice. The results showed that there was no free T-2 toxin in shrimp during 20 d accumulation toxicity test of T-2 toxin. And low-dose shrimp had no effect on mice. However, the high dose group shrimp caused an increase in neutrophils in mice, and erythrocyte system and platelet were significantly decreased (P<0.05). Moreover, serum biochemical indicators were significantly different (P<0.05). The damage of the head to the mice was higher than that of the muscle. Research showed that there was masked T-2 toxin in the infected shrimp, which had immune toxicity and blood toxicity to mice and led to the occurrence of inflammation, symptoms of anaemia, reduced the bone marrow hematopoietic function and damaged liver function. The damage of residual masked T-2 toxin in the head to the mice was higher than that of the muscle.

Key words: shrimp, masked T-2 toxin, routine blood, serum

中图分类号:

黄展锐, 王雅玲, 王小博, 莫冰, 邱妹, 孙力军, 陈康健, 刘健. 对虾中隐蔽态T-2毒素残留对小鼠血常规和血清生化指标的影响[J]. 浙江农业学报, 2017, 29(7): 1110-1118.

HUANG Zhanrui, WANG Yaling, WANG Xiaobo, MO Bing, QIU Mei, SUN Lijun, CHEN Kangjian, LIU Jian. Effect of masked T-2 toxin residues in Litopenaeus vannamei on routine blood and serum biochemical indexes of mice[J]. , 2017, 29(7): 1110-1118.

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对虾中隐蔽态T-2毒素残留对小鼠血常规和血清生化指标的影响

Effect of masked T-2 toxin residues in Litopenaeus vannamei on routine blood and serum biochemical indexes of mice

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