四川部分地区禽源空肠弯曲菌ERIC-PCR分型及毒力相关基因分析

doi:10.3969/j.issn.1004-1524.2017.09.07

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (9): 1465-1473.DOI: 10.3969/j.issn.1004-1524.2017.09.07

四川部分地区禽源空肠弯曲菌ERIC-PCR分型及毒力相关基因分析

王寒, 张博, 莫亚霞, 罗正中, 姜思讯, 孙成亮, 曹随忠, 姚学萍^*

四川农业大学动物医学院,动物疫病与人类健康四川省重点实验室,四川成都 611130

收稿日期:2017-03-15 出版日期:2017-09-20 发布日期:2017-09-27
通讯作者: 姚学萍,E-mail:yaoxueping74@126.com
作者简介:王寒(1991—),女,湖南永州人,硕士研究生,研究方向为动物临床分子诊断与治疗。E-mail:791390483@qq.com
基金资助:
四川农业大学学科建设双支计划(3572070); 四川省教育厅重点项目(15ZA0024)

ERIC-PCR typing and virulence-related genes detection of Campylobacter jejuni isolated from poultry in some areas of Sichuan Province

WANG Han, ZHANG Bo, MO Yaxia, LUO Zhengzhong, JIANG Sixun, SUN Chengliang, CAO Suizhong, YAO Xueping^*

College of Veterinary Medicine, Sichuan Agricultural University, Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China

Received:2017-03-15 Online:2017-09-20 Published:2017-09-27

摘要/Abstract

摘要： 为了解四川地区禽源空肠弯曲菌(Campylobacter jejuni)的遗传多样性和毒力相关基因的携带情况,对从四川成都、雅安及眉山市采集的鸡源、鸭源和鹌鹑源的盲肠及肛拭子样品进行C. jejuni的分离鉴定、ERIC-PCR分型和17种毒力相关基因的检测分析。结果显示,总体分离率为10.23%(48/469),其中鸡源分离率显著高于鹌鹑源(P<0.05)。ERIC-PCR聚类分析显示48株C. jejuni可分为6簇、16类共31种基因型,不同场分离株多在某一类或某一簇内聚集分布,而部分菌株在不同地区、不同场之间呈交叉分布。毒力基因检测结果显示,15种毒力相关基因的携带率在50%以上,其中鹌鹑源分离株的毒力基因携带率高于其他动物的携带率。由此表明四川地区禽源C. jejuni毒力基因的携带率高并且具有较高的遗传多样性。

关键词: 家禽, 空肠弯曲菌, ERIC-PCR分型, 毒力相关基因

Abstract: In order to investigate the genetic diversity and virulence related genes of Campylobacter jejuni in some areas of Sichuan, the samples of the cecum and anal swab collected from the chickens, ducks and quails from Chengdu, Ya'an and Meishan were isolated and identified for C. jejuni, and the ERIC-PCR typing and seventeen virulence-related genes were detected and analyzed. The results of isolation showed a 10.23% separation rate (48/469), and the isolation rate from chicken was higher than that from quail(P<0.05). ERIC-PCR cluster analysis showed that forty-eight strains of C. jejuni could be divided into six clusters, and there were sixteen species and thirty-one genotypes. The isolates from each farm were clustered and distributed in some species or cluster, while some isolates showed cross distribution in different regions and different farms. The carrying rate of fifteen virulence related genes was above 50%, and the carrying rate of virulence related genes of quail was higher than other animals. The results suggested that the C. jejuni isolates from poultry of Sichuan area possessed high carrying rate and high genetic diversity.

Key words: poultry, Campylobacter jejuni, ERIC-PCR typing, virulence-related genes

中图分类号:

S855.1⁺2

王寒, 张博, 莫亚霞, 罗正中, 姜思讯, 孙成亮, 曹随忠, 姚学萍. 四川部分地区禽源空肠弯曲菌ERIC-PCR分型及毒力相关基因分析[J]. 浙江农业学报, 2017, 29(9): 1465-1473.

WANG Han, ZHANG Bo, MO Yaxia, LUO Zhengzhong, JIANG Sixun, SUN Chengliang, CAO Suizhong, YAO Xueping. ERIC-PCR typing and virulence-related genes detection of Campylobacter jejuni isolated from poultry in some areas of Sichuan Province[J]. , 2017, 29(9): 1465-1473.

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四川部分地区禽源空肠弯曲菌ERIC-PCR分型及毒力相关基因分析

ERIC-PCR typing and virulence-related genes detection of Campylobacter jejuni isolated from poultry in some areas of Sichuan Province

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