EoNPV对灰茶尺蠖的致病特性及高效毒株筛选

doi:10.3969/j.issn.1004-1524.2017.10.13

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (10): 1686-1691.DOI: 10.3969/j.issn.1004-1524.2017.10.13

EoNPV对灰茶尺蠖的致病特性及高效毒株筛选

唐美君^{1, 2}, 郭华伟^{1, 2}, 葛超美¹, 殷坤山¹, 肖强^{1, 2}

1.中国农业科学院茶叶研究所, 浙江杭州 310008;
2.农业部茶叶质量安全控制重点实验室,浙江杭州 310008

收稿日期:2017-04-11 出版日期:2017-10-20 发布日期:2017-12-05
作者简介:唐美君(1972-),女,江苏丹徒人,推广研究员,主要从事茶树病虫防治技术研究。E-mail: tmjtea@tricaas.com
基金资助:
国家重点研发计划(2016YFD0200902); 浙江省公益性技术应用研究计划(2014C32095)

Pathogenic characters of Ectropis obliqua nucleopolyhedroviruses on Ectropis grisescens Warren and screening of high efficient strain

TANG Meijun^{1, 2}, GUO Huawei^{1, 2}, GE Chaomei¹, YIN Kunshan¹, XIAO Qiang^{1, 2}

1. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;
2. Key Laboratory of Tea Quality and Safety Control, Ministry of Agriculture, Hangzhou 310008, China

Received:2017-04-11 Online:2017-10-20 Published:2017-12-05

摘要/Abstract

摘要： 茶尺蠖核型多角体病毒(Ectropis obliqua nucleopolyhedronviruses,EoNPV)是茶尺蠖的重要天敌。灰茶尺蠖(Ectropis grisescens Warren)是茶尺蠖的近缘种,也是茶树主要害虫。为了探明应用EoNPV防控灰茶尺蠖的效果,发挥其对灰茶尺蠖的生物防治作用,通过生物测定,研究了EoNPV对灰茶尺蠖的致病特性,并进行了高效毒株的筛选。结果表明,EoNPV对灰茶尺蠖1龄幼虫致病力最强,2×10⁴~2×10⁸ PIB·mL^-1处理对1龄幼虫的致死率达82.7%~100%;对灰茶尺蠖2龄幼虫的LC₅₀值为2.32×10⁶ PIB·mL^-1 (饲毒后9 d);对不同代别灰茶尺蠖的致病力有明显差别,以第2代为最高。从用灰茶尺蠖连续增殖5代的病毒样品中,筛选出高效毒株QF₄,该毒株对灰茶尺蠖致死率比原始毒株提高51.5%,致死中时间缩短1.5 d。研究表明,应用EoNPV防治灰茶尺蠖最适宜在第2代1龄期施用;QF₄可作为病毒生产用毒株。

关键词: EoNPV, 灰茶尺蠖, 致病特性, 高效毒株

Abstract: Ectropis obliqua nucleopolyhedroviruses (EoNPV) is an effective pathogen of Ectropis obliqua Prout larvae. Ectropis grisescens Warren is a related species of E. obliqua, which is the major pest of tea plant. To investigate the using of EoNPV against E. grisescens, the pathogenic characters of EoNPV on E. grisescens and screening of high efficient strain were studied. The results showed the 1^st instar larvae of E. grisescens was the most sensitive to EoNPV, the mortality was 82.7%-100% infected with EoNPV in the concentration of 2×10⁴-2×10⁸ PIB·mL^-1. The value of LC₅₀ for the 2^nd instar larvae was 2.32×10⁶ PIB·mL^-1 on the 9^th day after inoculation. The virulence of EoNPV against the larvae of 2^nd generation of E. grisescens was highest among the 1^st-5^th generations. The strain QF₄ was screened as high efficient strain through bioassay among all the strains which was extracted from the dead larvae of E. grisescens infected by EoNPV constantly. The mortality rate of E. grisescens infected by this virus strain increased by 51.5% than that of original strain, and the median lethal time was shortened by 1.5 days. Thus it is suggested that the suitable period of spraying in the field could be at the 1^st instar larvae of 2^nd generations and the QF₄ stain could be used to multiply the virus.

Key words: EoNPV, Ectropis grisescens, pathogenic character, high efficient strain

中图分类号:

S435.711

唐美君, 郭华伟, 葛超美, 殷坤山, 肖强. EoNPV对灰茶尺蠖的致病特性及高效毒株筛选[J]. 浙江农业学报, 2017, 29(10): 1686-1691.

TANG Meijun, GUO Huawei, GE Chaomei, YIN Kunshan, XIAO Qiang. Pathogenic characters of Ectropis obliqua nucleopolyhedroviruses on Ectropis grisescens Warren and screening of high efficient strain[J]. , 2017, 29(10): 1686-1691.

参考文献

[1] 赵烨烽,侯建文. 茶尺蠖病毒病的观察测定与田间试验简报[J]. 中国茶叶,1980(3):14-17.
ZHAO Y F, HOU J W, A report on the observation and experiment in the field of the insect disease caused by Ectropis obliqua virus[J]. China Tea , 1980 (3): 14-17. (in Chinese)
[2] 陈棣华,张立人,刘军,等. 茶尺蠖核型多角体病毒研究[J]. 生物防治通报, 1989, 5(4): 168-172.
CHEN D H, ZHANG L R, LIU J, et al. A five-year study on the nuclear polyhedrosis virus of tea geometrid, Ectropis obliqua [Lep. Geometridae][J]. Chinese Journal of Biological Control , 1989, 5(4): 168-172. (in Chinese with English abstract)
[3] 叶恭银, 胡萃. 三种主要环境因子对茶尺蠖核型多角体病毒毒力的影响[J]. 应用生态学报, 1991, 2(3): 269-274.
YE G Y, HU C. Effect of three main environmental factors on virulence of NPV from tea geometrid[J]. Chinese Journal of Applied Ecology , 1991, 2(3): 269-274. (in Chinese with English abstract)
[4] 叶恭银, 胡萃, 朱俊庆, 等. 茶尺蠖核型多角体病毒对宿主种群的控制作用[J]. 植物保护学报, 1994, 21(3): 231-237.
YE G Y, HU C, ZHU J Q, et al. The control effect of tea geometrid nuclear polyhedrosis virus on host population[J]. Acta Phytophylacica Sinica , 1994, 21(3): 231-237. (in Chinese with English abstract)
[5] MA X C, XU H J, TANG M J, et al. Morphological, phylogenetic and biological characteristics of Ectropis obliqua single-nucleocapsid nucleopolyhedrovirus[J]. Journal of Microbiology , 2006, 44(1):77-82.
[6] 叶恭银, 胡萃. 适用于高温季节的茶尺蠖核型多角体病毒制剂的筛选[J]. 植物保护学报, 1991, 18(3): 215-220.
YE G Y, HU C. Screening for the nuclear polyhedrosis virus preparation of tea geometrid available in high temperature season[J]. Acta Phytophylacica Sinica , 1991, 18(3): 215-220. (in Chinese with English abstract)
[7] 殷坤山,陈华才,肖强,等. 茶尺蠖核型多角体病毒制剂的试制与推广应用[J].中国病毒学,2000,15(增刊1):81-84.
YIN K S, CHEN H C, XIAO Q, et al. Widespreading and application of Ectropic obliqua nuclear polyhedrosis virus(NPV) preparations[J]. Virologica Sinica , 2000,15(S1):81-84. (in Chinese with English abstract)
[8] 殷坤山,陈华才,唐美君,等. 茶尺蠖病毒杀虫剂田间使用技术的研究[J]. 中国病毒学, 2003,18(5):492-495.
YIN K S, CHEN H C, TANG M J, et al. Studies on application techniques of EoNPV preparations[J]. Virologica Sinica , 2003,18(5):492-495. (in Chinese with English abstract)
[9] 唐美君. 茶尺蠖病毒制剂生产与应用技术的研究及推广[D]. 杭州: 浙江大学,2008.
TANG M J. Studies on the production and application of EoNPV preparations for the management of the tea looper, Ectropis obliqua [D]. Hangzhou: Zhejiang University, 2008. (in Chinese with English abstract)
[10] 席羽. 茶尺蠖地理种群对茶尺蠖核型多角体病毒的敏感性差异及遗传变异研究[D]. 北京:中国农业科学院,2011.
XI Y. Susceptibility variation against Ectropis obliqua nucleopolyhedrovirus and genetic variation in geographic populations of tea geometrid, Ectropis obliqua Prout[D]. Beijing: Chinese Academy of Agricultural Sciences, 2011. (in Chinese with English abstract)
[11] 席羽,殷坤山,唐美君,等. 浙江茶尺蠖地理种群已分化成为不同种[J].昆虫学报,2014,57(9):1117-1122.
XI Y,YIN K S,TANG M J, et al. Geographic populations of the tea geometrid, Ectropis obliqua (Lepidoptera: Geometridae) in Zhejiang,eastern China have differentiated into different species[J]. Acta Entomologica Sinica ,2014,57(9):1117-1122. (in Chinese with English abstract)
[12] 葛超美,殷坤山,唐美君,等. 灰茶尺蠖的生物学特性[J].浙江农业学报,2016,28(3):464-468.
GE C M, YIN K S, TANG M J, et al. Biological characteristics of Ectropis grisescens Warren[J]. Acta Agriculturae Zhejiangensis , 2016,28(3):464-468. (in Chinese with English abstract)

EoNPV对灰茶尺蠖的致病特性及高效毒株筛选

Pathogenic characters of Ectropis obliqua nucleopolyhedroviruses on Ectropis grisescens Warren and screening of high efficient strain

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 3

编辑推荐

Metrics

本文评价

[1]	李红, 唐美君, 郭华伟, 王志博, 肖强. EoNPV对茶尺蠖两近缘种的毒力差异[J]. 浙江农业学报, 2020, 32(8): 1415-1419.
[2]	葛超美，殷坤山，唐美君，肖强*. 灰茶尺蠖的生物学特性[J]. 浙江农业学报, 2016, 28(3): 464-.
[3]	陈华才;王志岚;殷昆山. 茶尺蠖核型多角体病毒制剂的研制[J]. , 2006, 18(5): 0-316.