樟子松外生菌根形态类型及分子鉴定

doi:10.3969/j.issn.1004-1524.2017.10.12

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (10): 1678-1685.DOI: 10.3969/j.issn.1004-1524.2017.10.12

樟子松外生菌根形态类型及分子鉴定

张文泉¹, 罗国涛¹, 余洋², 闫伟^{3, *}

1.凯里学院,贵州凯里 556011;
2.中国地质环境监测院,北京 100081;
3.内蒙古农业大学林学院,内蒙古呼和浩特 010019

收稿日期:2017-06-14 出版日期:2017-10-20 发布日期:2017-12-05
通讯作者: 闫伟,E-mail:597139389@qq.com
作者简介:张文泉(1984-),男,湖南冷水江人,博士,副教授,主要从事林木生物技术方面的研究。E-mail: 445659248@qq.com
基金资助:
贵州省科技厅基础研究项目(黔科合基础[2017]1165号); 贵州省科技厅联合基金项目(黔科合LH字[2014]7241号); 国家自然科学基金项目(41504112); 贵州省科技厅联合基金项目(黔科合LH字[2015]7752号); 凯里学院博士专项课题(凯院合BS201339号)

Morphological type and molecular identification of ectomycorrhiza on Pinus sylvestris var. mongolica

ZHANG Wenquan¹, LUO Guotao¹, YU Yang², YAN Wei^{3, *}

1. Kaili University, Kaili 556011, China;
2. China Institute of Geo-Environment Monitoring, Beijing 100081, China;
3. College of Forestry, Inner Mongolia Agricultural University, Hohhot 010018, China

Received:2017-06-14 Online:2017-10-20 Published:2017-12-05

摘要/Abstract

摘要： 以内蒙古及周边地区樟子松的外生菌根为材料,采用形态解剖学与分子生物学手段相结合的方法,对与樟子松共生的外生菌根真菌多样性进行了全面调查及研究,并将所测序列在GenBank中进行比对。结果表明:在内蒙古及周边地区与樟子松共生的外生菌根真菌共19种,担子菌15种,子囊菌4种,分别来自于丝膜菌属(Cortinarius)、丝盖伞属(Inocybe)、牛肝属(Suillus)、蜡壳耳属(Sebacina)、红菇属(Russula)、乳菇属(Lactarius)、棉革菌属(Tomentella)、黏滑菇属(Hebeloma)和空团菌属(Cenococcum)、块菌属(Tuber)、口蘑属(Tricholoma)。表明樟子松外生菌根真菌多样性相对较高,且与利用地上子实体鉴定的外生菌根真菌种类有一定的区别。

关键词: 樟子松, 外生菌根, 形态解剖学, 分子鉴定, ITS

Abstract: The primary study on the diversity of ectomycorrhiza associated with Pinus sylvestris var mongolica in Inner Mongolia surrounding area was investigated using morphological, anatomical and molecular method, and the sequences obtained were blasted using GenBank. The results indicated that there were 19 species different ectomycorrhizas on Pinus sylvestris var. mongolica in Inner Mongolia area. Among these 19 species ectomycorrhizas fungi, 3 species belong to Ascomycotina and the rest species belong to Basidiomycotina, they belong respectively to Cortinarius, Inocybe, Suillus, Sebacina, Russula, Lactarius, Tomentella, Hebeloma, Cenococcum, Tuber, Tricholoma. The results showed that the diversity of ectomycorrhizas on Pinus sylvestris var. mongolica was high, and it was different with results identified by fruiting body associated with Pinus sylvestris var. mongolica.

Key words: Pinus sylvestris var. mongolica, ectomycorrhiza, morphological and anatomical characteristics, molecular identification, ITS

中图分类号:

S718.81

张文泉, 罗国涛, 余洋, 闫伟. 樟子松外生菌根形态类型及分子鉴定[J]. 浙江农业学报, 2017, 29(10): 1678-1685.

ZHANG Wenquan, LUO Guotao, YU Yang, YAN Wei. Morphological type and molecular identification of ectomycorrhiza on Pinus sylvestris var. mongolica[J]. , 2017, 29(10): 1678-1685.

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樟子松外生菌根形态类型及分子鉴定

Morphological type and molecular identification of ectomycorrhiza on Pinus sylvestris var. mongolica

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