苦瓜α-苦瓜素基因启动子克隆与单倍型分析

doi:10.3969/j.issn.1004-1524.2018.01.08

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (1): 58-64.DOI: 10.3969/j.issn.1004-1524.2018.01.08

苦瓜α-苦瓜素基因启动子克隆与单倍型分析

刘子记¹, 朱婕², 牛玉¹, 杨衍^{1, *}

1.中国热带农业科学院热带作物品种资源研究所/农业部华南作物基因资源与种质创制重点开放实验室,海南儋州 571737;
2.海南大学热带农林学院,海南海口 570228

收稿日期:2017-06-07 出版日期:2018-01-20 发布日期:2018-02-09
通讯作者: 杨衍,E-mail:catasvegetable@126.com
作者简介:刘子记(1982—),男,山东荷泽人,博士,副研究员,主要从事蔬菜分子生物学及遗传育种研究。E-mail:liuziji1982@163.com
基金资助:
中央级公益性科研院所基本科研业务费专项资金(1630032014019,1630032015015,1630032016019); 国家自然科学基金(31601758)

Cloning and haplotype analysis of α-momorcharin promoter in bitter gourd

LIU Ziji¹, ZHU Jie², NIU Yu¹, YANG Yan^{1, *}

1. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, P. R. China, Danzhou 571737, China;
2. Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China

Received:2017-06-07 Online:2018-01-20 Published:2018-02-09

摘要/Abstract

摘要： 为探明α-苦瓜素基因的表达调控规律,从苦瓜种质Y5中克隆了α-苦瓜素基因上游包括起始密码子在内的1 620 bp序列,选取转录起始位点上游1 500 bp序列,利用PlantCARE在线启动子预测工具进行分析。结果表明,α-苦瓜素启动子除了含有TATA-box和CAAT-box等核心元件外,还含有光响应元件、赤霉素响应元件、热胁迫响应元件、干旱应答元件、水杨酸应答元件、茉莉酸应答元件等。以28份苦瓜种质为材料,分析了α-苦瓜素启动子区域SNP和InDel分布情况,共发现24个SNP位点和1个InDel,28份苦瓜种质资源共存在4种单倍型。3个SNP位于转录因子结合位点,可能对α-苦瓜素基因的表达调控有重要作用。

关键词: 苦瓜, α, -苦瓜素, 启动子, 单倍型

Abstract: In order to explore the expression regulation of α-momorcharin gene, the upstream sequence, 1 620 bp in length including start codon of α-momorcharin gene was cloned in bitter gourd (Y5). The 1 500 bp upstream of the transcription start site was selected for analysis based on PlantCARE promoter prediction tools. The results showed that in addition to containing the core elements of TATA-box and CAAT-box, the α-momorcharin promoter also contained light response elements, gibberellin response element, heat stress response element, drought response element, salicylic acid response element, jasmonic acid response element etc. Taking 28 bitter gourd germplasm as materials, the SNP and InDel distribution in promoter region of α-momorcharin was analyzed. Twenty-four SNP loci and 1 InDel were found. Four haplotypes were identified in α-momorcharin gene promoter through SNP and InDel grouping. Three SNPs were located in transcription factor binding sites, which may play important roles in regulating the expression of α-momorcharin gene.

Key words: bitter gourd, α-momorcharin;, promoter, haplotype

中图分类号:

S652.9

刘子记, 朱婕, 牛玉, 杨衍. 苦瓜α-苦瓜素基因启动子克隆与单倍型分析[J]. 浙江农业学报, 2018, 30(1): 58-64.

LIU Ziji, ZHU Jie, NIU Yu, YANG Yan. Cloning and haplotype analysis of α-momorcharin promoter in bitter gourd[J]. , 2018, 30(1): 58-64.

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苦瓜α-苦瓜素基因启动子克隆与单倍型分析

Cloning and haplotype analysis of α-momorcharin promoter in bitter gourd

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