复合侵染的马铃薯花叶病病原诊断

doi:10.3969/j.issn.1004-1524.2018.01.13

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (1): 99-105.DOI: 10.3969/j.issn.1004-1524.2018.01.13

复合侵染的马铃薯花叶病病原诊断

宋西娇^{1, 2}, 谢礼^{1, 2}, 宣裕吉³, 王芳⁴, 严成其¹, 陈剑平^{1, *}

1.浙江省植物有害生物防控重点实验室—省部共建国家重点实验室培育基地,农业部植保生物技术重点实验室,浙江省农业科学院病毒与生物技术研究所,浙江杭州 310021;;
2.浙江省农业科学院公共实验平台,浙江杭州 310021;
3.宁波市鄞州区种子推广管理站,浙江宁波 315100;
4.宁波市农业科学院生物技术研究所,浙江宁波 315101

收稿日期:2017-06-19 出版日期:2018-01-20 发布日期:2018-02-09
通讯作者: 陈剑平,E-mail: jpchen2001@126.com
作者简介:宋西娇(1987—),女,山东临沂人,硕士,研究实习员,从事电镜服务工作。E-mail: songxijiao@yeah.net
基金资助:
宁波市重大科技项目(2015c110013)

Pathogenic diagnosis of synergistic infection of potato mosaic disease

SONG Xijiao^{1, 2}, XIE Li^{1, 2}, XUAN Yuji³, WANG Fang⁴, YAN Chengqi¹, CHEN Jianping^{1, *}

1. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Ministry of Agriculture Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
2. Public Lab, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3. Ningbo Yinzhou Seed Extention Station, Ningbo 315100, China;
4. Institute of Biotechnology, Ningbo Academy of Agricultural Sciences, Ningbo 315101, China

Received:2017-06-19 Online:2018-01-20 Published:2018-02-09

摘要/Abstract

摘要： 2015年在浙江宁波发现感病马铃薯植株具有马铃薯花叶病的典型症状,即植株矮化,叶片花叶、皱缩。透射电镜负染色发现,其叶片中含有长度约600~900和400 nm的线状病毒粒子。超薄切片发现,病毒主要集中在叶肉细胞,产生的内含体包括病毒样粒子聚集体、串珠-片层内含体和柱状内含体。串珠-片层内含体代表马铃薯X属特征,柱状内含体代表马铃薯Y属特征,表明可能存在复合侵染,并且2种不同病毒引起的内含体被发现于同一细胞。使用马铃薯Y属病毒通用引物以及针对马铃薯X病毒设计的特异性引物进行RT-PCR扩增及测序,明确其为马铃薯Y病毒(Potato virus Y,PVY)和马铃薯X病毒(Potato virus X,PVX)复合侵染。

关键词: 马铃薯花叶病, 马铃薯X病毒, 马铃薯Y病毒, 复合侵染, 病原诊断

Abstract: In 2015, an infected potato plant was found in Zhejiang Ningbo, which had the typical symptom of dwarf and mosaic leaf was thought to be a potato viral disease. Two kinds of linear virus particles with the typical length of 600-900 and 400 nm were observed under transmission electron microscopy by the negative staining of infected potato leaves. Ultra-thin section revealed that viral products were accumulated in the mesophyll cells. Except for observing the virus like particles in cytoplasm, two other kinds of inclusion bodies could also be observed in one cell including the cylindrical inclusion (CI) body and the laminated inclusion component (LIC), which was induced by two kinds of different viruses, CI induced by Potyvirus and LIC induced by Potexvirus, implying a doubly infected in this plant. To verify the pathogen, a molecular assay was performed by RT-PCR and sequencing, by using of the universal primers of Potyvirus and the specific primers of Potexvirus. At last, with the positive results of pathogenic diagnosis, we confirmed that this sick potato was induced by a synergistic infection with two viruses of potato virus X(PVX) and potato virus Y (PVY).

Key words: potato mosaic disease, potato virus X, potato virus Y, synergitic infection, pathogenic diagnosis

中图分类号:

Q336
S41-30

宋西娇, 谢礼, 宣裕吉, 王芳, 严成其, 陈剑平. 复合侵染的马铃薯花叶病病原诊断[J]. 浙江农业学报, 2018, 30(1): 99-105.

SONG Xijiao, XIE Li, XUAN Yuji, WANG Fang, YAN Chengqi, CHEN Jianping. Pathogenic diagnosis of synergistic infection of potato mosaic disease[J]. , 2018, 30(1): 99-105.

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复合侵染的马铃薯花叶病病原诊断

Pathogenic diagnosis of synergistic infection of potato mosaic disease

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