碱蓬根系嗜盐耐盐真菌的分离与鉴定

doi:10.3969/j.issn.1004-1524.2018.04.17

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (4): 649-655.DOI: 10.3969/j.issn.1004-1524.2018.04.17

碱蓬根系嗜盐耐盐真菌的分离与鉴定

吴曰福^{1, 2}, 顾爱星¹, 王洪凯^{2, *}

1.新疆农业大学农学院,新疆乌鲁木齐 830052;
2.浙江大学生物技术研究所,浙江杭州310058

收稿日期:2017-08-15 出版日期:2018-04-20 发布日期:2018-04-19
通讯作者: 王洪凯,E-mail:hkwang@zju.edu.cn
作者简介:吴曰福(1995—),男,江西鄱阳人,植物保护专业本科生。E-mail:1648590732@qq.com
基金资助:
浙江省自然科学基金(LY15C140001); 科技基础性工作专项(2014FY120900)

Isolation and identification of halophilic and halotolerant fungi from roots of Suaeda plants

WU Yuefu^{1, 2}, GU Aixing¹, WANG Hongkai^{2, *}

1.College of Agronomy, Xinjiang Agricultutal University, Urumqi 830052, China;
2. Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China

Received:2017-08-15 Online:2018-04-20 Published:2018-04-19

摘要/Abstract

摘要： 为分析耐盐植物碱蓬根系真菌的组成和耐盐程度,采用常规植物组织真菌分离方法对盐碱地中生长健康的碱蓬根系进行真菌分离,将分离得到的真菌在不同NaCl浓度培养基上进行培养,检测菌株的嗜盐耐盐性,并对分离得到的嗜盐耐盐真菌进行形态学鉴定和分子生物学鉴定。结果显示:分离得到耐盐真菌40株,分别属于镰刀菌属(Fusarium sp.)、链格孢属(Alternaria sp.)、曲霉属(Aspergillus sp.)、腐霉属(Pythium sp.)等,其中,棘孢曲霉(Aspergillus aculeatus)和尖孢镰刀菌(Fusarium oxysporum)是没有被报道过的耐盐真菌;分离得到嗜盐真菌3株,都属于聚多曲霉(Aspergillus sydowii)。结果表明,耐盐植物碱蓬中嗜盐耐盐真菌多样性丰富。

关键词: 嗜盐真菌, 耐盐真菌, 碱蓬, 真菌多样性

Abstract: In the present study, the traditional tissue-isolation method was used to isolate fungi from suaeda roots collected from Inner Mongolian for investigation of fungi composition structure and their salinity tolerance. Salinity tolerance of fungi were evaluated by fungal incubating method on PDA media with different concentrations of NaCl. The halophilic and halotolerant fungi were screened out and identified via both morphological characters and ITS rDNA sequencing. A total of 40 halotolerant fungi strains were isolated in this study, and were preliminary classified as Fusarium sp., Alternaria sp., Aspergillus sp., Pythium sp., etc. Among these fungi, Aspergillus aculeatus, Fusarium oxysporum were newly recorded halotolerant fungi. Three halophilic fungi were isolated and all were classified as Aspergillus sydowii. Results of the present research indicated that thediversity of salinity-tolerance fungi was high in the halotolerant plant rhizosphere.

Key words: halophilic fungi, halotolerant fungi, suaeda, fungal diversity

中图分类号:

Q938.2

吴曰福, 顾爱星, 王洪凯. 碱蓬根系嗜盐耐盐真菌的分离与鉴定[J]. 浙江农业学报, 2018, 30(4): 649-655.

WU Yuefu, GU Aixing, WANG Hongkai. Isolation and identification of halophilic and halotolerant fungi from roots of Suaeda plants[J]. , 2018, 30(4): 649-655.

参考文献

[1] GUNDE-CIMERMAN N, RAMOS J, PLEMENITAŠ A. Halotolerant and halophilic fungi[J]. Mycological Research , 2009, 113(11): 1231-1241.
[2] SMOLYANYUK E V, BILANENKO E N. Communities of halotolerant micromycetes from the areas of natural salinity[J]. Microbiology , 2011, 80(6): 877-883.
[3] GUNDE-CIMERMAN N, ZALAR P, DE HOOG S, et al. hypersaline waters in salterns-natural ecological niches for halophilic black yeasts[J]. FEMS Microbiology Ecology , 2000, 32(3): 235-240.
[4] 李艳华. 嗜盐与耐盐微生物的分离与系统分类[D]. 保定: 河北大学, 2003.
LI Y H. Separation and classification system of halophilic and halotolerant microorganism[D]. Baoding: Hebei University, 2013. (in Chinese with English abstract)
[5] 代东梅. 嗜盐耐盐真菌的分类及分子系统学研究[D]. 泰安: 山东农业大学, 2013.
DAI D M. Classification and molecular systematics study of halophilic and halotolerant microorganism[D]. Tai'an: Shandong Agricultural University, 2013. (in Chinese with English abstract)
[6] MIN Y J, PARK M S, FONG J J, et al. Diversity and saline resistance of endophytic fungi associated with Pinus thunbergii in coastal shelterbelts of Korea[J]. Journal of Microbiology and Biotechnology , 2014, 24(3): 324-333.
[7] ZALAR P, HOOG G S, SCHROERS H J, et al. Taxonomy and phylogeny of the xerophylic genus Wallemia (Wallemiomycetes and Wallemiales, cl. et ord. nov.)[J]. Antonie van Leeuwenhoek , 2005, 87(4): 311-328.
[8] BELANGER P A, BEAUDIN J, ROY S, et al. High-through put screening of microbial adaptation to environmental stress[J]. Journal of Microbiological Methods , 2011, 85(2): 92-97.
[9] LEHMANN M, SCHWARZLAENDER M, OBATA T, et al. The metabolic response of Arabidopsis roots to oxidative stress is distinct from that of heterotrophic cells in culture and highlights a complex relationship between the levels of transcripts, metabolites, and flux[J]. Molecular Plant , 2009, 2(3): 390-406.
[10] RADHAKRISHNAN R, KANG S M, BAEK I Y, et al. Characterization of plant growth-promoting traits of Penicillium species against the effects of high soil salinity and root disease[J]. Journal of Plant Interactions , 2014, 9(1): 754-762.
[11] LOPEZ-RAEZ J A. How drought and salinity affect arbuscular mycorrhizal symbiosis and strigolactone biosynthesis?[J]. Planta , 2016, 243(6): 1375-1385.
[12] 孙玉芳, 宋福强, 常伟, 等. 盐碱胁迫下AM真菌对沙枣苗木生长和生理的影响[J]. 林业科学, 2016, 52(6): 18-27.
SUN Y F, SONG F Q, CHANG W, et al. Effect of arbuscular mycorrhizal fungi on growth and physiology of Elaegnus angustifolia seedlings subjected to salinity stress[J]. Scientia Silvae Sinicae , 2016, 52 (6): 18-27. (in Chinese with English abstract)
[13] MANI K, SALGAONKAR B B, DAS D, et al. Community solar salt production in Goa, India[J]. Aquatic Biosystems , 2012,8(1): 30.
[14] 韦娜. 嗜盐菌的分离鉴定及其应用研究[D]. 青岛: 中国海洋大学, 2012.
WEI N. Separation, identification and its application research of halophilic microorganism[D]. Qingdao: Ocean University of China, 2012. (in Chinese with English abstract)
[15] 赵可夫, 李法曾, 樊守金, 等. 中国的盐生植物[J]. 植物学通报, 1999, 16(3): 201-207.
ZHAO K F, LI F Z, FAN S J, et al. Halophytes in China[J]. Chinese Bulletin of Botany , 1999, 16(3): 201-207. (in Chinese with English abstract)
[16] FLOWERS T J, COLMER T D. Salinity tolerance in halophytes[J]. New Phytologist , 2008, 179(4): 945-963.
[17] ZHANG D, YANG Y, CASTLEBURY L A, et al. A method for the large scale isolation of high transformation efficiency fungal genomic DNA[J]. FEMS Microbiology Letters , 1996, 145(2): 261-265.
[18] 魏景超. 真菌鉴定手册[M]. 上海: 上海科学技术出版社,1979.
[19] 邵力平, 沈祥瑞, 张素轩, 等. 真菌分类学[M]. 北京: 中国农业出版社, 1984.
[20] KIS-PAPO J, GRISHKAN I, AHARON O, et al. Spatiotemporal diversity of filamentous fungi in the hypersaline Dead Sea[J]. Mycological Research , 2001, 105(6): 749-756.
[21] 丁小维. 盐蒿内生耐(嗜)盐真菌的分离与鉴定[J]. 陕西理工学院学报(自然科学版), 2014, 30(4): 55-60.
DING X W. Isolation and identification of endophytic halotolerant and halophilic fungi associated with Artemisia halodendron [J]. Journal of Shaanxi University of Technology ( Natural Science Edition ), 2014, 30(4): 55-60. (in Chinese with English abstract)
[22] 李涛. AM真菌对不同盐生植物抗盐性的影响[D]. 济南: 山东师范大学, 2009.
LI T. The effect of AM fungi on different salt-tolerant plants' salt resistance[D]. Jinan: Shandong Normal University, 2009. (in Chinese with English abstract)
[23] GARCIA I V, MENDOZA R E. Arbuscular mycorrhizal fungi and plant symbiosis in a saline-sodic soil[J]. Mycorrhiza , 2007, 17(3): 167-174.
[24] 陈亚平. 耐盐碱植物内生真菌的分离鉴定及促进作物耐盐菌株的筛选[D]. 杭州: 浙江大学, 2014.
CHEN Y P. Isolation and identification of endophytic fungi in saline plants and screening on its strengthening crop's salt tolerance[D]. Hangzhou: Zhejiang University, 2014. (in Chinese with English abstract)
[25] MACIÁ-VICENTE J G, FERRARO V, BURRUANO S, et al. Fungal assemblages associated with roots of halophytic and non-halophytic plant species vary differentially along a salinity gradient[J]. Microbial Ecology , 2012, 64(31): 668-679.
[26] 黄菁菁, 鲁春华, 钱晓呜, 等. 台湾海峡海洋耐(嗜)盐真菌及其抗菌活性的初步研究[J]. 厦门大学学报(自然科学版), 2008, 47(5): 723-727.
HUANG J J, LU C H, QIAN X M, et al. Study on halophilic and haloptolerant fungi from Taiwan Strait and their antimicrobial activities[J]. Journal of Xiamen University ( Natural Science ), 2008, 47(5): 723-727. (in Chinese with English abstract)

碱蓬根系嗜盐耐盐真菌的分离与鉴定

Isolation and identification of halophilic and halotolerant fungi from roots of Suaeda plants

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