四川部分地区断奶前犊牛贾第虫感染调查和基因分型研究

doi:10.3969/j.issn.1004-1524.2018.07.03

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (7): 1116-1121.DOI: 10.3969/j.issn.1004-1524.2018.07.03

四川部分地区断奶前犊牛贾第虫感染调查和基因分型研究

但佳明¹, 欧红萍², 严光文³, 田一男¹, 魏斌¹, 涂蕊¹, 肖启程¹, 唐丽¹, 杨亭玉¹, 彭广能¹, 王永刚⁴, 钟志军^{1, *}

1.四川农业大学动物医学院动物疫病与人类健康四川省重点实验室,四川成都 611130;
2.成都农业科技职业学院,四川成都 611130;
3.西昌学院动物科技学院,四川西昌 615000;
4.北川羌族自治县曲山镇畜牧兽医站,四川绵阳 622750

收稿日期:2017-09-25 出版日期:2018-07-20 发布日期:2018-08-02
通讯作者: 钟志军,E-mail: zhongzhijun488@126.com
作者简介:但佳明(1995—),女,四川自贡人,硕士研究生,从事兽医临床病理学与分子诊断学研究。E-mail:542378229@qq.com
基金资助:
国家重点研发计划(2016YFD0501009); 国家自然科学基金(31000548); 成都大熊猫繁育研究基金会项目(CPF2015-4); 四川农业大学本科生科研兴趣培养项目(ky2016076)

Prevalence and genotyping of Giardia duodenalis in pre-weaned dairy calves in Sichuan Province

DAN Jiaming¹, OU Hongping², YAN Guangwen³, TIAN Yinan¹, WEI Bin¹, TU Rui¹, XIAO Qicheng¹, TANG Li¹, YANG Tingyu¹, PENG Guangneng¹, WANG Yonggang⁴, ZHONG Zhijun^{1, *}

1.Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine,Sichuan Agricultural University, Chengdu 611130, China;
2. Chengdu Agricultural College,Chengdu 611130, China;
3. College of Animal Science and Technology, Xichang College, Xichang 615000, China;
4. Veterinary Station of Beichuan Qiang Autonomous County, Mianyang 622750, China

Received:2017-09-25 Online:2018-07-20 Published:2018-08-02

摘要/Abstract

摘要： 为调查四川部分地区断奶前犊牛贾第虫感染和基因亚型分布情况,采集了278份断奶前犊牛(1月龄以下)新鲜粪便,采用饱和蔗糖漂浮法处理样品并提取DNA,经巢式PCR扩增β-giaidin(bg)、tpi和gdh基因,对阳性样品贾第虫基因型进行鉴定。结果显示,四川部分地区断奶前犊牛贾第虫总感染率为9.35%(26/278)。bg、tpi和gdh基因序列分析表明,本次分离的贾第虫种类均为集聚体E。多位点基因序列和进化树分析显示,基于bg、tpi和gdh三个基因位点均分别鉴定出7种亚型,分别发现3、2、2种新基因亚型(bg: E13、E14、E16;tpi: E21、E24;gdh: E18、E19)。本研究表明,四川地区断奶前犊牛存在贾第虫感染,虫种类型主要为集聚体E,同时该型在四川地区遗传多样性较高。

关键词: 贾第虫, 断奶前犊牛, 集聚体E

Abstract: To investigate the prevalence and molecular characteristics of Giardia duodenalis (G. duodenalis) in pre-weaned dairy calves in Sichuan Province, a total of 278 pre-weaned dairy calves' fecal samples were randomly collected in Sichuan. Samples were examined by the Sheather's sucrose flotation technique, and DNA was extracted. These samples were detected for Giardia by nest PCR amplification of β-giardin (bg), tpi and gdh genes. The results showed that 9.35% (26/278) was positive for G. duodenalis infection. One assemblage was detected, which was assemblage E. Multilocus genotyping showed that 7 subtypes of assemblage including 3, 2 and 2 new subtypes (bg: E13, E14, E16; tpi: E21, E24; gdh: E18, E19) were observed based on bg, tpi and gdh genes, respectively. The results indicated that assemblage E had the ascendant position in pre-weaned dairy calves in Sichuan Province and which showed its high genetic diversity.

Key words: Giardia duodenalis, pre-weaned dairy calves, assemble E

中图分类号:

S852.7

但佳明, 欧红萍, 严光文, 田一男, 魏斌, 涂蕊, 肖启程, 唐丽, 杨亭玉, 彭广能, 王永刚, 钟志军. 四川部分地区断奶前犊牛贾第虫感染调查和基因分型研究[J]. 浙江农业学报, 2018, 30(7): 1116-1121.

DAN Jiaming, OU Hongping, YAN Guangwen, TIAN Yinan, WEI Bin, TU Rui, XIAO Qicheng, TANG Li, YANG Tingyu, PENG Guangneng, WANG Yonggang, ZHONG Zhijun. Prevalence and genotyping of Giardia duodenalis in pre-weaned dairy calves in Sichuan Province[J]. , 2018, 30(7): 1116-1121.

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四川部分地区断奶前犊牛贾第虫感染调查和基因分型研究

Prevalence and genotyping of Giardia duodenalis in pre-weaned dairy calves in Sichuan Province

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