泥鳅蛋白抗氧化肽的Plastein反应修饰研究

doi:10.3969/j.issn.1004-1524.2018.08.06

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (8): 1312-1320.DOI: 10.3969/j.issn.1004-1524.2018.08.06

泥鳅蛋白抗氧化肽的Plastein反应修饰研究

高丹丹¹, 程浩¹, 马忠仁^1,2, 田晓静¹, 李明生^1,2, 陈士恩¹, 常坤朋¹, 刘根娣^1,*, 娜扎瑞尔·亚哈亚³

1.西北民族大学生命科学与工程学院,甘肃兰州 730124;
2.甘肃省动物细胞工程技术研究中心,甘肃兰州 730030;
3.马来西亚伊斯兰理科大学理工学院,马来西亚森美兰州尼来新区 71800

收稿日期:2017-11-06 出版日期:2018-08-25 发布日期:2018-08-28
通讯作者: 刘根娣,E-mail: mymawanyun@163.com
作者简介:高丹丹(1983—),女,河南汝州人,博士,副教授,研究方向为食品生物技术。E-mail:gaodan0322@163.com
基金资助:
科技部援助项目(KY201501005); 西北民族大学中央高校基本科研业务费专项资金(31920120028); 甘肃省科技计划资助项目 (17YF1WA166); 甘肃省兰州市城关区科技计划 (2017KJGG0024); “长江学者和创新团队发展计划”创新团队(IRT_17R88); 国家自然科学基金(31360375)

Modification of antioxidant peptide from loach protein by Plastein reaction

GAO Dandan¹, CHENG Hao¹, MA Zhongren^1,2, TIAN Xiaojing¹, LI Mingsheng^1,2, CHEN Shi'en¹, CHANG Kunpeng¹, LIU Gendi^1,*, NAZARIYAH Yahaya³

1.College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730124, China;
2. Engineering Technology Research Center for Animal Cell, Lanzhou 730030, China;
3.Faculty of Science and Technology, Universiti Sains Islam Malaysia, Bandar Baru Nilai , Nilai, Negeri Sembilan 71800, Malaysia

Received:2017-11-06 Online:2018-08-25 Published:2018-08-28

摘要/Abstract

摘要： 采用中性蛋白酶水解泥鳅蛋白制备抗氧化肽,以DPPH自由基清除能力为指标,用木瓜白酶对泥鳅蛋白抗氧化肽进行Plastein反应修饰研究。通过单因素试验研究了外源氨基酸种类、pH值、时间、E/S、温度和外源氨基酸的添加量对泥鳅蛋白抗氧化肽Plastein反应修饰的影响,在此基础上采用响应面法对泥鳅蛋白抗氧化肽Plastein反应修饰工艺进行了优化。结果表明,泥鳅蛋白抗氧化肽Plastein反应修饰的最佳条件为:底物浓度40%、组氨酸添加量为0.5 mmol·g^-1、E/S为1 782.49 U·g^-1 、pH值9.0、温度35 ℃、时间3 h,此条件下制备的Plastein反应修饰产物的抗氧化活性是修饰前的1.99倍,其对DPPH自由基的清除率为(77.98±0.08)%。

关键词: 泥鳅蛋白, 抗氧化肽, Plastein反应, 响应面

Abstract: Loach protein was hydrolyzed by neutrase to prepare antioxidant peptide in this study. Plastein reaction was used to modify loach protein hydrolysates for enhancing the scavenging ability of DPPH free radical of peptides by papain. The influences of the types of added amino acids, the extrinsic glycine dose, the substrate/enzyme ratio, pH value, temperature and hydrolysis time on scavenging ability of DPPH free radical of the Plastein reaction modification of loach protein hydrolysates were investigated by using single factor analysis method. On this basis, the optimal technology conditions for Plastein reaction modification of antioxidant peptide from loach protein was obtained by response surface methodology. Results showed that the optimal conditions for Plastein reaction modification of antioxidant peptide from loach protein were as followings: substrate concentration at 40%, extrinsic dose of histidine at 0.5 mmol·g^-1, E/S ratio at 1 782.49 U·g^-1, pH at 9.0, temperature at 35 ℃, and reaction time at 3.0 h. The antioxidant activity of modified antioxidant peptide by Plastein reaction was about 1.99 times of original antioxidant peptide under this condition, and the scavenging ability of DPPH free radical was (77.98±0.08)%.

Key words: loach protein, antioxidant peptide, Plastein reaction, response surface

中图分类号:

S98
TS201.2

高丹丹, 程浩, 马忠仁, 田晓静, 李明生, 陈士恩, 常坤朋, 刘根娣, 娜扎瑞尔·亚哈亚. 泥鳅蛋白抗氧化肽的Plastein反应修饰研究[J]. 浙江农业学报, 2018, 30(8): 1312-1320.

GAO Dandan, CHENG Hao, MA Zhongren, TIAN Xiaojing, LI Mingsheng, CHEN Shi'en, CHANG Kunpeng, LIU Gendi, NAZARIYAH Yahaya. Modification of antioxidant peptide from loach protein by Plastein reaction[J]. , 2018, 30(8): 1312-1320.

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泥鳅蛋白抗氧化肽的Plastein反应修饰研究

Modification of antioxidant peptide from loach protein by Plastein reaction

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