恩诺沙星在大口黑鲈(Micropterus salmoides)体内代谢动力学研究

doi:10.3969/j.issn.1004-1524.2018.09.06

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (9): 1482-1488.DOI: 10.3969/j.issn.1004-1524.2018.09.06

恩诺沙星在大口黑鲈(Micropterus salmoides)体内代谢动力学研究

何欣¹, 王扬^{2, *}, 贝亦江²

1.杭州市农业科学研究院实验中心,浙江杭州 310024;
2.浙江省水产技术推广总站,浙江杭州 310012

收稿日期:2017-12-19 出版日期:2018-09-25 发布日期:2018-10-15
作者简介:何欣(1986—),男,浙江舟山人,博士,从事农产品质量与安全研究。E-mail: ahe0839@163.com

Pharmacokinetics of enrofloxacin in largemouth bass (Micropterus salmoides)

HE Xin¹, WANG Yang^{2, *}, BEI Yijiang²

1. Testing Center, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China;
2. Zhejiang Fishery Technology Extension Center, Hangzhou 310012, China

Received:2017-12-19 Online:2018-09-25 Published:2018-10-15
Contact: 王扬,E-mail: 1752315020@qq.com
Supported by:
浙江省水产品质量安全技术支撑团队(QS2016012); 杭州市农科院创新基金(2018HNCX-06); 浙江省农业新品种选育重大科技专项重点研发计划(2016C02055-3)

摘要/Abstract

摘要： 给大口黑鲈(Micropterus salmoides)连续5 d口服30 mg·kg^-1剂量(以体质量计)的恩诺沙星,利用液相色谱技术测定不同时间点大口黑鲈各组织中恩诺沙星的含量,建立药时曲线,探讨恩诺沙星在大口黑鲈体内的代谢动力学。结果表明:在(25±2)℃水温下,大口黑鲈血液、肌肉、肝脏中恩诺沙星的达峰时间均为4 h,吸收半衰期分别为0.998、2.176、1.892 h,消除半衰期分别为8.494、23.84、37.53 h,血液中的药时曲线下总面积为132.23 μg·mL^-1·h,肌肉和肝脏中的药时曲线下总面积分别为273.50、247.12 μg·g^-1·h。25 ℃下,恩诺沙星在大口黑鲈体内的建议休药期为15 d。

关键词: 恩诺沙星, 大口黑鲈, 代谢动力学

Abstract: To investigate the metabolic rate of enrofloxacin in largemouth bass (Micropterus salmoides) and facilitate dosage decisions, the pharmacokinetics of enrofloxacin in largemouth bass were studied after 5 d fed with 30 mg·kg^-1 (oral doses, based on body weight) enrofloxacin under (25±2) ℃. The concentrations of enrofloxacin in largemouth bass were determined by high performance liquid chromatography, and the drug-time curves were calculated. It was shown that the peak times of enrofloxacin in blood, muscle and liver were all 4 h under the experiment conditions. The absorption half lives of enrofloxacin in blood, muscle and liver were 0.998, 2.176, 1.892 h, respectively, and elimination half lives were 8.494, 23.84, 37.53 h, respectively. The area under drug concentration-time curves were 132.23 μg·mL^-1·h in plasma and 273.50, 247.12 μg·g^-1·h, respectively, in muscle and liver. Recommended withdrawal period of enrofloxacin in largemouth bass was 15 d under 25 ℃.

Key words: enrofloxacin, largemouth bass, pharmacokinetics

中图分类号:

S948

何欣, 王扬, 贝亦江. 恩诺沙星在大口黑鲈(Micropterus salmoides)体内代谢动力学研究[J]. 浙江农业学报, 2018, 30(9): 1482-1488.

HE Xin, WANG Yang, BEI Yijiang. Pharmacokinetics of enrofloxacin in largemouth bass (Micropterus salmoides)[J]. , 2018, 30(9): 1482-1488.

参考文献

[1] 中华人民共和国农业部.中华人民共和国农业部第235号公告—动物性食品中兽药最高残留限量[A]. 北京:中华人民共和国农业部,2002.
[2] 邓显文,谢芝勋,刘加波,等. 嗜水气单胞菌、爱德华氏菌灭活菌苗免疫保护及药物治疗试验[J]. 广西农业科学,2010,41(3):266-269.
DENG X W, XIE Z X, LIU J B, et al. Protective immunity in tilapia against inactivated bacterin of Aeromonas hydrophila and Edwardsiella and controlling effects of antibacterial medicines[J]. Guangxi Agricultural Sciences , 2010, 41(3):266-269. (in Chinese with English abstract)
[3] PARK S W. Studies on classification of Korean catfish ( Silurus asotus ) peripheral leucocytes[J]. Journal of Clinical Nursing , 1995, 8(1):296-297.
[4] 李雪峰,王利. 鲈鱼摩氏摩根菌的鉴定及药敏试验[J]. 动物医学进展, 2015, 36(2):66-68.
LI X F, WANG L. Identification and drug sensitivity test of Morganella morganii isolated from Lateolabrax japonicus [J]. Progress in Veterinary Medicine , 2015, 36(2):66-68. (in Chinese)
[5] 简纪常,吴灶和, 陈刚. 恩诺沙星在眼斑拟石首鱼体内的药物代谢动力学[J].中国兽医学报, 2005, 25(2):195-197.
JIAN J C, WU Z H, CHEN G. Pharmacokinetics of enrofloxacin in Sciaenops ocellatus [J]. Chinese Journal of Veterinary Science , 2005, 25(2):195-197. (in Chinese with English abstract)
[6] 李佳蔚,刘慧慧,于召强,等. 恩诺沙星在黑鲪体内的药物代谢动力学研究[J]. 中国渔业质量与标准, 2012, 2(1):52-57.
LI J W, LIU H H, YU Z Q, et al. Pharmacokinetics of enrofloxacin in Sebastodes fuscescens [J]. Chinese Fishery Quality and Standards ,2012, 2(1):52-57. (in Chinese with English abstract)
[7] 方星星,刘秀红,丁相卿,等. 恩诺沙星在异育银鲫体内的组织分布及消除规律[J]. 南京农业大学学报, 2012, 35 (4):110-114.
FANG X X, LIU X H, DING X Q, et al. Distribution and elimination of enrofloxacin in allogynogenetic silver crucian carp, Carassius auratus gibelio [J]. Journal of Nanjing Agricultural University , 2012, 35 (4):110-114. (in Chinese with English abstract)
[8] 房文红,于慧娟,蔡友琼,等. 恩诺沙星及其代谢物环丙沙星在欧洲鳗鲡体内的代谢动力学[J]. 中国水产科学, 2007, 14(4):622-629.
FANG W H, YU H J, CAI Y Q, et al. Pharmacokinetics of enrofloxacin and its metabolite ciprofloxacin in European eel ( Anguilla anguilla ) after a single oral gavage[J]. Journal of Fishery Sciences of China , 2007, 14(4):622-629. (in Chinese with English abstract)
[9] 郭娇娇,潘红艳,杨虎,等. 恩诺沙星在杂交鲟体内的药物代谢动力学[J]. 大连海洋大学学报, 2011, 26(4):362-366.
GUO J J, PAN H Y, YANG H, et al. Pharmacokinetics of enrofloxacin in sturgeon Acipenser schrenckii [J]. Journal of Dalian Fisheries University , 2011, 26(4):362-366. (in Chinese with English abstract)
[10] OKOUR M, BRUNDAGE R C. Modeling enterohepatic circulation[J]. Current Pharmacology Reports , 2017:1-13.
[11] 张德云,匡维华,郑映钦. 恩诺沙星在日本鳗鲡体内残留消除规律研究[J]. 水产科学,2007,26(4):210-213.
ZHANG D Y, KUANG W H, ZHENG Y Q. Studies on elimination of enrofloxacin residues and its metabolites in eel tissues[J]. Fisheries Science , 2007, 26 (4):210-213. (in Chinese with English abstract)
[12] LEWBART G, VADEN S, DEEN J, et al. Pharmacokinetics of enrofloxacin in the red pacu ( Colossoma brachypomum ) after intramuscular, oral and bath administration[J]. Journal of Veterinary Pharmacology & Therapeutics. 1997, 20 (2):124-128.
[13] FORREST A, NIX D E, BALLOW C H, et a1.Pharmacok dynamic of intravenous ciprofloxacin in seriously ill patients[J]. Antimicrob Agents Chemother , 1993, 37(5):1073-1081.
[14] MARRA F, PARTOVI N, JEWESSON P. Aminoglycoside administrations as a single daily dose: an improvement to current practice or a repeat of previous errors[J]. Drugs , 1996, 52(3):344-370.
[15] 徐丽娟. 恩诺沙星控制淡水鱼类嗜水气单胞菌性败血症的防耐药用药方案研究[D]. 上海:上海海洋大学,2014.
XU L J. Establishing the medication regimen for prevention of drug resistance using enrofloxacin against Aeromonas hydrophila in freshwater [D]. Shanghai: Shanghai Ocean University,2014. (in Chinese with English abstract)
[16] 房文红,周凯. 诺氟沙星对溶藻弧菌和恩诺沙星对迟缓爱德华菌的抗生素后效应[J]. 海洋渔业, 2005, 27(1):44-48.
FANG W H, ZHOU K. Postantibiotic effects of norfloxacin and enrofloxacin on some pathogenic bacteria from aquatic animals[J]. Marine Fisheries , 2005, 27(1):44-48. (in Chinese with English abstract)
[17] 马寅,金珊,余开,等. 恩诺沙星对4种水产致病弧菌的抑杀菌效应[J]. 微生物学通报, 2011, 38(8):1216-1221.
MA Y, JIN S, YU K, et al. Pharmacodynamics effect of enrofloxacin on four aquatic pathogenic vibrio[J]. Microbiology , 2011, 38(8):1216-1221. (in Chinese with English abstract)

恩诺沙星在大口黑鲈(Micropterus salmoides)体内代谢动力学研究

Pharmacokinetics of enrofloxacin in largemouth bass (Micropterus salmoides)

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