浙江农业学报 ›› 2018, Vol. 30 ›› Issue (9): 1476-1481.DOI: 10.3969/j.issn.1004-1524.2018.09.05

• 动物科学 • 上一篇    下一篇

白介素21在42日龄中国黄羽鹌鹑盲肠扁桃体中的定位

李琴, 何敏*, 赵飘, 陈小梅, 吕莹莹   

  1. 四川农业大学 动物医学院,四川 成都 611130
  • 收稿日期:2018-01-19 出版日期:2018-09-25 发布日期:2018-10-15
  • 作者简介:李琴(1995—),女,四川达州人,本科生。E-mail: alger1024@163.com

Immunohistochemical localization of interleukin-21 in cecal tonsil of 42 day old Chinese Yellow Feather quail

LI Qin, HE Min*, ZHAO Piao, CHEN Xiaomei, LYU Yingying   

  1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
  • Received:2018-01-19 Online:2018-09-25 Published:2018-10-15
  • Contact: 何敏,E-mail: hemin09@163.com

摘要: 为探明白介素21(IL-21)在鹌鹑机体免疫调节的作用机制,观察IL-21的分布特点,取42日龄中国黄羽鹌鹑盲肠扁桃体,运用免疫组织化学法对白介素21进行组织学定位,观察阳性细胞的形态及分布情况。结果显示,IL-21阳性反应细胞多呈圆形或椭圆形,少数呈梭形,有的呈团块状,阳性反应细胞主要分布于黏膜上皮下固有层中,少数位于肠腺周围,生发中心周围也有少量阳性细胞分布。证实IL-21在鹌鹑盲肠扁桃体中广泛存在,分布数量及反应强度与局部免疫细胞及机体免疫状态有关。

关键词: 白介素21, 中国黄羽鹌鹑, 盲肠扁桃体, 定位

Abstract: To investigate the mechanism of interleukin-21 in the immune regulation of the Chinese Yellow Feather quail body, the distribution characteristics of IL-21 were observed. The quail tonsils of 42 day old Chinese Yellow Feather quail were taken, immunohistochemical SABC method was used for histological localization of interleukin-21 in the tonsil, and the morphology and distribution of the IL-21positive cells were observed. The results showed that the IL-21 positive reaction cells were round or elliptic, and a few were fusiform, some were clumpy. The positive reaction cells were mainly distributed in the lamina propria of the underlying layer of the epithelium of cecal tonsil, and a few were around the intestinal gland. There was also a small amount of IL-21 positive cells around the germinal centers. The results confirmed that IL-21 was widely distributed in the cecal tonsil of quail. The distribution and reaction strength of IL-21 positive cells were related to the local immune cells and the immune state of the body.

Key words: interleukin-21, Chinese Yellow Feather quail, cecal tonsil, localization

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