浙江农业学报 ›› 2025, Vol. 37 ›› Issue (4): 808-819.DOI: 10.3969/j.issn.1004-1524.20240596

• 园艺科学 • 上一篇    下一篇

番茄SlMYB52基因鉴定、亚细胞定位及表达分析

狄延翠(), 嵇泽琳, 王媛媛, 娄世浩, 张涛, 国志信, 申顺善, 朴凤植, 杜南山, 董晓星, 董韩*()   

  1. 河南农业大学 园艺学院,河南 郑州 450046
  • 收稿日期:2024-07-04 出版日期:2025-04-25 发布日期:2025-05-09
  • 作者简介:狄延翠( 2000—),女,河南三门峡人,硕士研究生,研究方向为设施蔬菜优质高效生产技术。E-mail:13525866912@163.com
  • 通讯作者: *董韩,E-mail:440069@henau.edu.cn
  • 基金资助:
    国家自然科学基金(32202579);河南省科技攻关项目(242102111075);中国博士后科学基金面上项目(2022M711062)

Identification, subcellular localization and expression analysis of tomato SlMYB52 gene

DI Yancui(), JI Zelin, WANG Yuanyuan, LOU Shihao, ZHANG Tao, GUO Zhixin, SHEN Shunshan, PIAO Fengzhi, DU Nanshan, DONG Xiaoxing, DONG Han*()   

  1. College of Horticulture, Henan Agricultural University, Zhengzhou 450046, China
  • Received:2024-07-04 Online:2025-04-25 Published:2025-05-09

摘要: MYB转录因子在植物逆境应答过程中发挥着重要的调控作用。为了挖掘更多番茄(Solanum lycopersicum L.)MYB转录因子成员信息,本研究从番茄中克隆了SlMYB52基因,采用生物信息学手段对SlMYB52基因进行基因结构、编码蛋白信息、保守结构域、系统进化树及启动子顺式作用元件预测等的分析,利用烟草叶片瞬时转化法分析亚细胞定位,通过qRT-PCR进行组织特异性表达及响应逆境胁迫的分析。结果表明SlMYB52基因全长1 431 bp,编码253个氨基酸,预测相对分子质量为29 035.22,理论等电点9.08,属于不稳定蛋白质、亲水性蛋白质;SlMYB52所编码蛋白质不含跨膜结构,没有潜在的信号肽位点;该蛋白质二级结构以无规卷曲为主,占比59.68%;系统进化树分析显示,SlMYB52与NtMYB4a亲缘关系最近;亚细胞定位结果显示,该SlMYB52蛋白定位在细胞核;对SlMYB52启动子分析,发现其含有大量的逆境响应元件;qRT-PCR结果表明,SlMYB52基因在茎中表达最高,其次是叶,在顶芽中表达量最低;SlMYB52基因的表达受高盐、低温及辣椒疫霉菌侵染的诱导,在干旱胁迫下SlMYB52基因的表达受到明显抑制,说明该基因可能参与逆境胁迫的应答。本研究结果为进一步研究SlMYB52基因的生物学功能及作用机制提供理论依据。

关键词: 番茄, MYB转录因子, 逆境胁迫, 亚细胞定位, 生物信息学分析

Abstract:

MYB transcription factors play an important regulatory role in plant stress response. In order to explore more information about MYB transcription factor members in tomato (Solanum lycopersicum L.), the SlMYB52 gene was cloned from tomato, and its gene structure, coding protein information, conserved domain, phylogenetic tree, and promoter cis-acting element prediction were analyzed using bioinformatics methods, the subcellular localization of SlMYB52 was analyzed using transient transformation in tobacco leaves, and tissue-specific expression and stress response were analyzed using qRT-PCR in this study. The results showed that SlMYB52 gene was 1 431 bp in length, encoding 253 amino acids, with a predicted relative molecular weight of 29 035.22 and a theoretical isoelectric point of 9.08, belonging to unstable proteins and hydrophilic proteins. The protein encoded by SlMYB52 has no transmembrane structure and no potential signal peptide site. The secondary structure of the protein was maily composed of random coli, accounting for 59.68%. Phylogenetic tree analysis showed that the amino acid sequence of SlMYB52 and NtMYB4a was closely related. Subcellular localization results showed that the SlMYB52 protein was located in the nucleus. The analysis of the SlMYB52 promoter revealed that it contained a large number of stress response elements. Moreover, the results of qRT-PCR showed that the expression of SlMYB52 gene was highest in stem, followed by leaves, and lowest in apical bud. Furthermore, the expression of SlMYB52 gene was induced by high salt, low temperature, and Phytophthora capsici infection, and was inhibited by drought stress, indicating that this gene may be involved in stress response. The results of this study provide a theoretical basis for further studying the biological function and mechanism of SlMYB52 gene.

Key words: tomato, MYB transcription factor, stress, subcellular localization, bioinformatics analysis

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