丝瓜高密度bin标记遗传图谱构建与果长QTL定位

doi:10.3969/j.issn.1004-1524.2023.03.12

浙江农业学报 ›› 2023, Vol. 35 ›› Issue (3): 590-597.DOI: 10.3969/j.issn.1004-1524.2023.03.12

丝瓜高密度bin标记遗传图谱构建与果长QTL定位

崔竣杰(), 吕振, 杨天文, 王静, 洪宇, 曹毅^*()

佛山科学技术学院园艺系,广东佛山 528225

收稿日期:2022-05-05 出版日期:2023-03-25 发布日期:2023-04-07
通讯作者: ^*曹毅,E-mail:762122849@qq.com
作者简介:崔竣杰(1987—),男,四川巴中人,博士,讲师,研究方向为蔬菜种质创新与遗传育种。E-mail:seedcui@sina.com
基金资助:
佛山市植物种质资源工程技术研究中心项目(BKS208024)

Construction of high-density bin marker genetic map and QTL mapping for fruit length in Luffa spp.

CUI Junjie(), LYU Zhen, YANG Tianwen, WANG Jing, HONG Yu, CAO Yi^*()

Department of Horticulture, Foshan University, Foshan 528225, Guangdong, China

Received:2022-05-05 Online:2023-03-25 Published:2023-04-07

摘要/Abstract

摘要：

为探究丝瓜高密度遗传图谱构建、挖掘果长变异遗传位点,以果长差异显著的有棱丝瓜自交系盛优长丝瓜和普通丝瓜自交系苹果丝瓜为亲本,构建包含67个植株的BC₁群体,利用全基因组重测序进行bin标记基因分型并构建丝瓜高密度种间遗传图谱。该图谱总共包含9 299个bin标记,分布于14个连锁群上,总遗传距离为2 956.57 cM(约覆盖普通丝瓜80.47%基因组区域),平均遗传距离为0.32 cM。结合该遗传图谱,运用MapQTL 6软件中MQM模型进行丝瓜果长QTL检测,在连锁群Scaf 1的标记scaf1-bin33(19.76 cM)和scaf1-bin359(92.84 cM)之间定位到1个丝瓜果长性状的QTL位点qFL1.1,其LOD值为4.21,贡献率为28.90%,加性效应为6.04。研究结果为基于丝瓜种间遗传图谱的性状遗传挖掘提供理论依据。

关键词: 丝瓜, bin标记, 种间遗传图谱, 果长, QTL定位

Abstract:

To explore the construction of a high-density genetic map of Luffa spp. and to identify the genetic loci for fruit length variation, a BC₁ population of 67 individuals was developed by crossing two inbred lines, Luffa acutangula ‘Shengyou’ and L. cylindrica ‘Pingguo’, with significant differences in fruit length. A high-density interspecific genetic map of Luffa spp. was constructed using the bin markers based on whole genome resequencing. The map consisted of 9 299 bin markers distributed in 14 linkage groups spanning 2 956.57 cM (about 80.47% of the Luffa cylindrica genome) with an average genetic distance of 0.32 cM. Combined with the genetic map, the MQM model in MapQTL 6 software was used to detect QTL for fruit length in Luffa spp. A QTL locus qFL1.1 for fruit length in Luffa spp. was mapped between markers scaf1-bin33 (19.76 cM) and scaf1-bin359 (92.84 cM) on linkage group Scaf 1. The LOD of qFL1.1 was 4.21, the contribution rate was 28.90% and the additive effect was 6.04. The results provided a theoretical basis for genetic mining based on the interspecific genetic map in Luffa spp.

Key words: Luffa spp., bin marker, interspecific genetic map, fruit length, QTL mapping

中图分类号:

S642.4

崔竣杰, 吕振, 杨天文, 王静, 洪宇, 曹毅. 丝瓜高密度bin标记遗传图谱构建与果长QTL定位[J]. 浙江农业学报, 2023, 35(3): 590-597.

CUI Junjie, LYU Zhen, YANG Tianwen, WANG Jing, HONG Yu, CAO Yi. Construction of high-density bin marker genetic map and QTL mapping for fruit length in Luffa spp.[J]. Acta Agriculturae Zhejiangensis, 2023, 35(3): 590-597.

图/表 6

参考文献 30

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材料 Sample	平均值 Mean/cm	标准差 Standard deviation	标准误 Standard error of the mean	正态分布W检验(P) Normal distribution W test (P)	偏度 Skewness	峰度 Kurtosis
盛优长丝瓜Shengyou	62.70	5.06	1.69	0.20	-0.44	-0.09
苹果丝瓜Pingguo	13.63	0.76	0.25	0.98	-0.06	-1.88
F₁	41.04	2.06	0.69	0.59	-0.39	-0.95
BC₁	19.76	5.65	0.75	0.02	0.83	-0.13

材料 Sample	平均值 Mean/cm	标准差 Standard deviation	标准误 Standard error of the mean	正态分布W检验(P) Normal distribution W test (P)	偏度 Skewness	峰度 Kurtosis
盛优长丝瓜Shengyou	62.70	5.06	1.69	0.20	-0.44	-0.09
苹果丝瓜Pingguo	13.63	0.76	0.25	0.98	-0.06	-1.88
F₁	41.04	2.06	0.69	0.59	-0.39	-0.95
BC₁	19.76	5.65	0.75	0.02	0.83	-0.13

连锁群 Linkage groups	标记数量 Number of markers	连锁群长度 Linkage group length/cM	平均遗传距离 Average distance/cM	覆盖基因组大小 Genome coverage/Mb	重组率(cM/Mb) Recombination rate (cM/Mb)
Scaf 1	948	318.34	0.34	56.71	5.61
Scaf 2	1 365	310.58	0.23	51.04	6.08
Scaf 3	1 097	283.53	0.26	49.46	5.73
Scaf 4	417	98.44	0.24	48.32	2.04
Scaf 5	700	258.13	0.37	48.36	5.34
Scaf 6	509	333.61	0.66	48.61	6.86
Scaf 7	1 035	248.23	0.24	48.37	5.13
Scaf 8	771	291.90	0.38	45.43	6.43
Scaf 9	127	35.70	0.28	4.81	7.42
Scaf 10-1	130	25.98	0.20	4.62	5.62
Scaf 10-2	153	21.25	0.14	4.11	5.17
Scaf 11	729	275.49	0.38	44.99	6.12
Scaf 12	538	242.54	0.45	41.69	5.82
Scaf 13	780	212.85	0.27	41.82	5.09
总数Total	9 299	2 956.57	0.32	538.33	5.49

连锁群 Linkage groups	标记数量 Number of markers	连锁群长度 Linkage group length/cM	平均遗传距离 Average distance/cM	覆盖基因组大小 Genome coverage/Mb	重组率(cM/Mb) Recombination rate (cM/Mb)
Scaf 1	948	318.34	0.34	56.71	5.61
Scaf 2	1 365	310.58	0.23	51.04	6.08
Scaf 3	1 097	283.53	0.26	49.46	5.73
Scaf 4	417	98.44	0.24	48.32	2.04
Scaf 5	700	258.13	0.37	48.36	5.34
Scaf 6	509	333.61	0.66	48.61	6.86
Scaf 7	1 035	248.23	0.24	48.37	5.13
Scaf 8	771	291.90	0.38	45.43	6.43
Scaf 9	127	35.70	0.28	4.81	7.42
Scaf 10-1	130	25.98	0.20	4.62	5.62
Scaf 10-2	153	21.25	0.14	4.11	5.17
Scaf 11	729	275.49	0.38	44.99	6.12
Scaf 12	538	242.54	0.45	41.69	5.82
Scaf 13	780	212.85	0.27	41.82	5.09
总数Total	9 299	2 956.57	0.32	538.33	5.49

丝瓜高密度bin标记遗传图谱构建与果长QTL定位

Construction of high-density bin marker genetic map and QTL mapping for fruit length in Luffa spp.

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