棘胸蛙白内障病原鉴定及病理组织观察

doi:10.3969/j.issn.1004-1524.2018.11.02

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (11): 1811-1818.DOI: 10.3969/j.issn.1004-1524.2018.11.02

棘胸蛙白内障病原鉴定及病理组织观察

刘子明¹, 金晶², 胡则辉³, 戴庆敏¹, 李巧玲¹, 施倩⁴, 吕耀平^1,2,*

1.丽水学院生态学院,浙江丽水 323000;
2.宁波大学海洋学院,浙江宁波 315211;
3.浙江省海洋水产研究所,浙江舟山 316021;
4.丽水市中心医院,浙江丽水 323000

收稿日期:2018-03-13 出版日期:2018-11-25 发布日期:2018-12-21
通讯作者: 吕耀平,E-mail: yaopinglv@163.com
作者简介:刘子明(1986—),男,福建晋江人,博士,讲师,主要从事水生生物学研究。E-mail: lziming1986@163.com
基金资助:
国家自然科学基金(31500308);浙江省自然科学基金(Y3110477);浙江省一流学科生态学(浙教高科〔2016〕169号);浙江省新苗人才计划(2015R429016);浙江省丽水市科技局项目(20150407)

Pathogen isolation and identification of cataract disease from Chinese spiny frog (Quasipaa spinosa ) and its histological observation

LIU Ziming¹, JIN Jing², HU Zehui³, DAI Qingmin¹, LI Qiaoling¹, SHI Qian⁴, LYU Yaoping^1,2,*

1. College of Ecology, Lishui University, Lishui 323000, China;
2. School of Marine Sciences, Ningbo University, Ningbo 315211, China;
3. Marine Fishery Institute of Zhejiang Province, Zhoushan 316021, China;
4. Lishui Central Hospital, Lishui 323000, China

Received:2018-03-13 Online:2018-11-25 Published:2018-12-21

摘要/Abstract

摘要： 为了确定棘胸蛙(Quasipaa spinosa)白内障病的病原,进行了病原菌分离、人工感染、分离菌理化特性和16S rDNA基因序列分析。结果表明,从患病棘胸蛙分离出2种致病菌Mm1和Mm2,将其大腿肌肉注射感染健康棘胸蛙,表现出与自然发病蛙相似症状。通过理化特性与16S rDNA基因序列鉴定,分离菌为摩氏摩根菌(Morganella morganii),其对链霉素和庆大霉素高度敏感,对氨苄西林和头孢他啶产生抗药性。病理组织学观察发现,其感染可引起棘胸蛙白内障病,发病棘胸蛙的晶状体、肝、肠和肾损伤严重,出现组织溶解、核溶解等现象,且在肠部出现包涵体。本研究发现摩氏摩根菌可感染棘胸蛙,导致棘胸蛙白内障病,并初步研究了病原菌的生物学特性、病理特征和药物敏感性,以期为该病的诊断和防治提供理论依据。

关键词: 棘胸蛙, 白内障病, 摩氏摩根菌, 组织病理, 药敏试验

Abstract: To define the pathogen of cataract disease from Chinese spiny frog (Quasipaa spinosa), the pathogen isolation, artificial infection, physical and chemical characteristics of isolated bacteria and sequence analysis of 16S rDNA were analyzed. The results showed that two pathogens (Mm1 and Mm2) were isolated from the diseased frogs. Healthy Chinese spiny frogs injected the isolated intramuscularly exhibited the similar clinical signs, gross and histopathological lesions to the naturally infected frogs. The isolated strains were identified to be Morganella morganii according to physicochemical properties and the 16S rDNA gene sequence analysis. The strains were sensitive to streptomycin and gentamycin, but resistant to ampicillin and cephalosporin. Histopathological observation found that Morganella morganii infection in Chinese spiny frogs could cause pathological damages, especially in the crystalline lens, liver, intestine, and kidney, accompanied by the emergence of karyolysis, histolysis, and inclusion body in intestine. This study found that Chinese spiny frogs could be infected with Morganella morganii and lead to cataract disease. Biological and pathological characteristics, drug sensitivities were analyzed. These results could provide theoretical basis for the diagnosis and treatment of cataracts disease of Chinese spiny frog.

Key words: Quasipaa spinose, cataract disease, Morganella morganii, histopathology, drug sensitivity test

中图分类号:

S947.2

刘子明, 金晶, 胡则辉, 戴庆敏, 李巧玲, 施倩, 吕耀平. 棘胸蛙白内障病原鉴定及病理组织观察[J]. 浙江农业学报, 2018, 30(11): 1811-1818.

LIU Ziming, JIN Jing, HU Zehui, DAI Qingmin, LI Qiaoling, SHI Qian, LYU Yaoping. Pathogen isolation and identification of cataract disease from Chinese spiny frog (Quasipaa spinosa ) and its histological observation[J]. , 2018, 30(11): 1811-1818.

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棘胸蛙白内障病原鉴定及病理组织观察

Pathogen isolation and identification of cataract disease from Chinese spiny frog (Quasipaa spinosa ) and its histological observation

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