鳖源嗜水气单胞菌的耐药性研究

doi:10.3969/j.issn.1004-1524.2017.09.04

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (9): 1445-1450.DOI: 10.3969/j.issn.1004-1524.2017.09.04

鳖源嗜水气单胞菌的耐药性研究

朱凝瑜, 郑晓叶, 曹飞飞, 李杨阳, 郑天伦^*

浙江省水产技术推广总站, 浙江杭州 310023

收稿日期:2017-02-16 出版日期:2017-09-20 发布日期:2017-09-27
通讯作者: 郑天伦,E-mail:nballan@163.com
作者简介:朱凝瑜(1984—),女,浙江长兴人,硕士,工程师,从事水产养殖病害研究。E-mail:zny1984@sina.com
基金资助:
浙江省重大科技专项重点农业项目(2015C02028-1); 浙江省水产品质量安全技术支撑团队项目(QS2016014)

Antimicrobial resistance of Aeromonas hydrophila isolated from soft-shelled turtle Pelodiscus sinensis

ZHU Ningyu, ZHENG Xiaoye, CAO Feifei, LI Yangyang, ZHENG Tianlun^*

Zhejiang Fisheries Technical Extension Center, Hangzhou 310023, China

Received:2017-02-16 Online:2017-09-20 Published:2017-09-27

摘要/Abstract

摘要： 对10株分离自患病中华鳖的嗜水气单胞菌进行了耐药性研究。药敏试验结果表明,这10株嗜水气单胞菌呈现相近的耐药谱,对阿米卡星、新霉素、头孢哌酮、氨曲南、多西环素、诺氟沙星、恩诺沙星等7种药物均敏感,对青霉素G、林可霉素、万古霉素、甲氧嘧啶等4种药物耐药,对氟苯尼考、四环素、复方新诺明产生了不同的耐药性。同时对qnrA、qnrB、qepA等17种耐药基因进行检测,10株菌均检测到了2~11种耐药基因,检出率最高的耐药基因为qnrA与cat 1,达到了100%,而qepA、aph(3')、sul Ⅱ、sul Ⅲ、OXA等5个基因未检出。同种细菌不同分离株表现出不同的耐药性,提示用药时有必要针对病原进行药敏试验,筛选敏感药物进行疾病防治。

关键词: 中华鳖, 嗜水气单胞菌, 药敏试验, 耐药基因

Abstract: The antimicrobial resistance of 10 Aeromonas hydrophila strains collected from diseased soft-shelled turtle Pelodiscus sinensis was studied in this paper. Drug sensitive tests showed that all the 10 strains were sensitive to Amikacin, Neomycin, Cefoperazone, Aztreonam, Deoxytetracycline, Norfloxacin, Enrofloxacin and were risistent to Penicillin G, Lincomycin, Vancomycin, Sulfamethoxydiazine, but they showed different susceptibility to Florfenicol, Tetracycline, SMZ-TMP. At the same time, 17 antimicrobial resistant genes in the 10 strains were detected by PCR. The results showed that all these 10 strains carried 2-11 resistance genes. The highest detection rate of resistant gene qnrA and cat 1 reached 100% , while qepA, aph(3'), sul Ⅱ, sul Ⅲ, OXA were not detected. Different Aeromonas hydrophila strains showed different antimicrobial resistance, suggesting that it was necessary to carry out drug sensitivity tests before treating and preventing.

Key words: Pelodiscus sinensis, Aeromonas hydrophila, drug sensitive tests, antimicrobial resistant gene

中图分类号:

朱凝瑜, 郑晓叶, 曹飞飞, 李杨阳, 郑天伦. 鳖源嗜水气单胞菌的耐药性研究[J]. 浙江农业学报, 2017, 29(9): 1445-1450.

ZHU Ningyu, ZHENG Xiaoye, CAO Feifei, LI Yangyang, ZHENG Tianlun. Antimicrobial resistance of Aeromonas hydrophila isolated from soft-shelled turtle Pelodiscus sinensis[J]. , 2017, 29(9): 1445-1450.

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鳖源嗜水气单胞菌的耐药性研究

Antimicrobial resistance of Aeromonas hydrophila isolated from soft-shelled turtle Pelodiscus sinensis

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