黄颡鱼溶血性腹水病初探

doi:10.3969/j.issn.1004-1524.2019.08.04

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (8): 1239-1248.DOI: 10.3969/j.issn.1004-1524.2019.08.04

黄颡鱼溶血性腹水病初探

杨移斌^1,2,3, 艾晓辉^2,3, 宋怿³, 董靖^2,3, 胥宁^2,3, 姜兰^1,*

1.中国水产科学研究院珠江水产研究所,广东广州 510380;
2.中国水产科学研究院长江水产研究所,湖北武汉 430223;
3.农业农村部水产品质量安全控制重点实验室,北京 100141

收稿日期:2019-02-26 出版日期:2019-08-25 发布日期:2019-08-30
通讯作者: 姜兰,E-mail: jianglan2@tom.com
作者简介:杨移斌(1988-),男,江西抚州人,博士,主要从事水产动物疾病学研究。E-mail: yangyb1988@126.com
基金资助:
(原)农业部渔用药物创制重点实验室开放课题(201707); 现代农业产业技术体系专项(CARS-49)

Preliminary study on hemolytic ascites disease of Pelteobagrus fulvidraco

YANG Yibin^1,2,3, AI Xiaohui^2,3, SONG Yi³, DONG Jing^2,3, XU Ning^2,3, JIANG Lan^1,*

1. Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China;
2. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China;
3. Key Laboratory of Control of Quality and Safety for Aquatic Products, Minisrty of Agriculture and Rural Affairs, Beijing 100141, China

Received:2019-02-26 Online:2019-08-25 Published:2019-08-30

摘要/Abstract

摘要： 从濒死黄颡鱼腹水中分离到一株细菌HS01,对分离株理化特性及DNA序列进行检测,根据理化特性、16S rRNA和gyrB基因序列比对,分离株HS01为嗜水气单胞菌。人工感染该菌后发病鱼出现与自然发病类似症状,并表现强毒力,对黄颡鱼的半致死剂量为2.51×10⁵ CFU·g^-1,且从病灶中分离到与感染菌一致菌株,因此确定嗜水气单胞菌是此次黄颡鱼溶血性腹水病的病原。分离株对头孢唑肟、头孢噻肟、奈替米星及氟苯尼考等10种抗生素高度敏感;对头孢拉定、链霉素、新霉素及卡那霉素等6种抗生素中度敏感;对阿莫西林、青霉素、妥布霉素及红霉素这4种抗生素耐药。本研究为黄颡鱼溶血性腹水病防控提供了理论依据。

关键词: 黄颡鱼, 嗜水气单胞菌, 分离鉴定, 病理, 高通量测序

Abstract: Bacterial strain HS01 was isolated from diseased Pelteobagrus fulvidraco suffered from serious hemolytic ascites, and it was identified as Aeromonas hydrophila based on biochemical reaction characters and 16S rRNA and gyrB gene sequence analyses. Artificial affected fish by intraperitoneal HS01-injection showed the same clinic signs as the diseased fish in ponds. Therefore, the isolated A.hydrophila was proved as the main pathogen, and its LD₅₀ to tilapia was determined as 2.51×10⁵ CFU·g^-1 using the probabilistic graphical method. The antibiotic sensitivity test showed that the isolated was very sensitive to cefotaximee, cefotaxime, netilmicin, florfenicol, etc., less sensitive to cefadroxil, streptomycin1, neomycin, kanamycin, etc., and not sensitive to amoxicillin, penicillin, tobramycin, erythrocin. This study provided a theoretical basis for the prevention and control of the hemorrhagic disease caused by A.hydrophila in Pelteobagrus fulvidraco.

Key words: Pelteobagrus fulvidraco, Aeromonas hydrophila, isolation and identification, pathology, 16S rDNA high-throughput sequencing technology

中图分类号:

S941.42

杨移斌, 艾晓辉, 宋怿, 董靖, 胥宁, 姜兰. 黄颡鱼溶血性腹水病初探[J]. 浙江农业学报, 2019, 31(8): 1239-1248.

YANG Yibin, AI Xiaohui, SONG Yi, DONG Jing, XU Ning, JIANG Lan. Preliminary study on hemolytic ascites disease of Pelteobagrus fulvidraco[J]. , 2019, 31(8): 1239-1248.

参考文献

[1] 丁瑞华. 四川鱼类志[M]. 成都: 四川科学技术出版社, 1994.
[2] 王素霞. 黄颡鱼的人工养殖技术[J]. 江西水产科技, 2007(3): 39-40.
WANG S X.Cultural culture of bagrid fish[J]. Jiangxi Fishery Sciences and Technology, 2007(3): 39-40.(in Chinese)
[3] 石征宇, 易弋, 韩书煜, 等. 黄颡鱼迟钝爱德华菌的分离鉴定及其致病性研究[J]. 中国畜牧兽医, 2018, 45(3): 807-813.
SHI Z Y, YI Y, HAN S Y, et al.Isolation and identification of Edwardsiella tardain Pelteobagrus fulvidraco and analysis of its pathogenicity[J]. China Animal Husbandry & Veterinary Medicine, 2018, 45(3): 807-813.(in Chinese with English abstract)
[4] 丁正峰, 薛晖, 边文冀, 等. 养殖黄颡鱼腹水症病原研究[J]. 华中农业大学学报, 2008, 27(5): 639-643.
DING Z F, XUE H, BIAN W J, et al.Pathogenic bacteria causing ascites disease of cultured yellow catfish(Pelteobagrus fulvidraco)[J]. Journal of Huazhong Agricultural University, 2008, 27(5): 639-643.(in Chinese with English abstract)
[5] 耿毅, 汪开毓, 范方玲, 等. 养殖黄颡鱼(Pelteobagrus fulvidraco)(鮰)爱德华氏菌(Edwardsiella ictaluri)的分离鉴定与生物学特性研究[J]. 海洋与湖沼, 2010, 41(1): 61-67.
GENG Y, WANG K Y, FAN F L, et al.Isolation, identification and biological characterization of Edwardsiella ictaluri from cultured yellow catfish Pelteobagrus fulvidraco[J]. Oceanologia et Limnologia Sinica, 2010, 41(1): 61-67.(in Chinese with English abstract)
[6] 胡宗云, 宋文华, 张涛, 等. 黄颡鱼“红头病”病原菌蜡状芽孢杆菌分离鉴定及药敏分析[J]. 水产学杂志, 2016, 29(4): 33-37.
HU Z Y, SONG W H, ZHANG T, et al.Identification and drug sensitivity test of pathogen Bacillus cereus from yellow catfish (Pelteobagrus fulvidraco) with red-head disease[J]. Chinese Journal of Fisheries, 2016, 29(4): 33-37.(in Chinese with English abstract)
[7] 徐洋, 蔺凌云, 姚嘉赟, 等. 黄颡鱼“溃疡综合征”病原的分离鉴定及药敏试验[J]. 淡水渔业, 2015, 45(5): 100-104.
XU Y, LIN L Y, YAO J Y, et al.Pathogen isolation, identification and susceptibility test of ulcerative disease syndrome on Pelteobagrus fulvidraco[J]. Freshwater Fisheries, 2015, 45(5): 100-104.(in Chinese with English abstract)
[8] 黄钧, 温华成, 施金谷, 等. 黄颡鱼体表溃疡病病原菌分离鉴定及药敏试验[J]. 南方农业学报, 2012, 43(1): 107-112.
HUANG J, WEN H C, SHI J G, et al.Isolation and identification of pathogenic bacteria from Pelteobagrus fulvidraco ulcerative syndrome and its drug sensitive test[J]. Journal of Southern Agriculture, 2012, 43(1): 107-112.(in Chinese with English abstract)
[9] 张玉蕾, 赵丽娟, 周伟东, 等. 黄颡鱼源柱状黄杆菌的分离鉴定及其对翘嘴鳜的致病性[J]. 华中农业大学学报, 2016, 35(2): 83-89.
ZHANG Y L, ZHAO L J, ZHOU W D, et al.Characterization and pathogenicity of a strain of Flavobacterium columnare isolated from Pelteobagrus fulvidraco[J]. Journal of Huazhong Agricultural University, 2016, 35(2): 83-89.(in Chinese with English abstract)
[10] 左跃, 易弋, 夏杰, 等. 2株黄颡鱼源类志贺邻单胞菌的分离与鉴定[J]. 江苏农业科学, 2013, 41(9): 199-201.
ZUO Y, YI Y, XIA J, et al.Isolation and identification of two strains of Pseudomonas shigella from fish[J]. Jiangsu Agricultural Sciences, 2013, 41(9): 199-201.(in Chinese)
[11] 杨移斌, 胥宁, 杨秋红, 等. 黄颡鱼源海豚链球菌分离、鉴定及药敏特性[J]. 浙江农业学报, 2017, 29(6): 903-909.
YANG Y B, XU N, YANG Q H, et al.Isolation, identification and antibiotic sensitivity of Streptococcus iniae from Pelteobagrus fulvidraco[J]. Acta Agriculturae Zhejiangensis, 2017, 29(6): 903-909.(in Chinese with English abstract)
[12] 陈成, 耿毅, 汪开毓, 等. 拟态弧菌感染黄颡鱼的动态病理损伤及病原分布研究[J]. 南方水产科学, 2017, 13(1): 10-18.
CHEN C, GENG Y, WANG K Y, et al.Dynamic distribution of Vibrio mimicus in infected yellow catfish (Pelteobagrus fulvidraco) and its hispathological changes[J]. South China Fisheries Science, 2017, 13(1): 10-18.(in Chinese with English abstract)
[13] 徐叔云. 药理实验方法学[M]. 北京: 人民卫生出版社, 2002.
[14] 东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京: 科学出版社, 2001.
[15] YAMAMOTO S, HARAYAMA S.PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains[J]. Applied and Environmental Microbiology, 1995, 61(3): 1104-1109.
[16] HEUZENROEDER M.Distribution of two hemolytic toxin genes in clinical and environmental isolates of Aeromonas spp.: correlation with virulence in a suckling mouse model[J]. FEMS Microbiology Letters, 1999, 174(1): 131-136.
[17] WANG G, CLARK C G, LIU C, et al.Detection and characterization of the hemolysin genes in Aeromonas hydrophila and Aeromonas sobria by multiplex PCR[J]. Journal of Clinical Microbiology, 2003, 41(3): 1048-1054.
[18] SEN K, RODGERS M.Distribution of six virulence factors in Aeromonas species isolated from US drinking water utilities: a PCR identification[J]. Journal of Applied Microbiology, 2004, 97(5): 1077-1086.
[19] 储卫华, 陆承平. 聚合酶链反应(PCR)法检测嗜水气单胞菌丝氨酸蛋白酶基因[J]. 中国兽医学报, 2003, 23(5): 463-464.
CHU W H, LU C P.Detection of the serine-protease gene in Aeromonas hydrophila by PCR[J]. Chinese Journal of Veterinary, 2003, 23(5): 463-464.(in Chinese with English abstract)
[20] 朱凝瑜, 郑晓叶, 曹飞飞, 等. 鳖源嗜水气单胞菌的耐药性研究[J]. 浙江农业学报, 2017, 29(9): 1445-1450.
ZHU N Y, ZHENG X Y, CAO F F, et al.Antimicrobial resistance of Aeromonas hydrophila isolated from soft-shelled turtle Pelodiscus sinensis[J]. Acta Agriculturae Zhejiangensis, 2017, 29(9): 1445-1450.(in Chinese with English abstract)
[21] 靳晓敏, 葛慕湘, 张艳英, 等. 不同来源嗜水气单胞菌耐药基因检测[J]. 淡水渔业, 2015, 45(3): 84-87, 92.
JIN X M, GE M X, ZHANG Y Y, et al.Detection of resistance genes in Aeromonas hydrophila strains[J]. Freshwater Fisheries, 2015, 45(3): 84-87, 92.(in Chinese with English abstract)
[22] WIKLER M A.Performance standards for antimicrobial susceptibility testing[M]. Washington: Clinical Laboratory Standards Institute, 2009.
[23] 夏围围, 贾仲君. 高通量测序和DGGE分析土壤微生物群落的技术评价[J]. 微生物学报, 2014, 54(12): 1489-1499.
XIA W W, JIA Z J.Comparative analysis of soil microbial communities by pyrosequencing and DGGE[J]. Acta Microbiologica Sinica, 2014, 54(12): 1489-1499. (in Chinese with English abstract)
[24] WILLIAMS S T, FOSTER P G, LITTLEWOOD D T J. The complete mitochondrial genome of a turbinid vetigastropod from MiSeq Illumina sequencing of genomic DNA and steps towards a resolved gastropod phylogeny[J]. Gene, 2014, 533(1): 38-47.
[25] 孙其焕, 孙佩芳, 金丽华, 等. 异育银鲫溶血性腹水病病原的研究[J]. 水产学报, 1991, 15(2): 130-139.
SUN Q H, SUN P F, JIN L H, et al.On the pathogenic bacteria of the hemolytic ascitesosis of allogynogenetic crucian carp[J]. Journal of Fisheries of China, 1991, 15(2): 130-139.(in Chinese with English abstract)
[26] 孙佩芳, 蔡完其. 中华鳖温和气单胞菌病的病原研究[J]. 淡水渔业, 1998, 28(4): 3-5.
SUN P F, CAI W Q.Study on the pathogen of Aeromonas sobria disease[J]. Freshwater Fisheries, 1998, 28(4): 3-5.(in Chinese)
[27] 李庆乐, 陆建仁. 鲢鳙鱼溶血型腹水病的防治研究[J]. 广西农业科学, 1994(5): 233-235.
LI Q L, LU J R.Study on the pathogen of Aeromonas sobria disease[J]. Guangxi Agricultural Sciences, 1994(5): 233-235.(in Chinese)
[28] 舒新华, 金燮理, 肖克宇, 等. 乌鳢腹水病病原的分离和鉴定[J]. 湖南农业大学学报, 1998, 24(4): 286-290.
SHU X H, JIN X L, XIAO K Y, et al.Identification and isolation of pathogenic bacteria of snake head's ascitesosis[J]. Journal of Hunan Agricultural University, 1998, 24(4): 286-290.(in Chinese with English abstract)
[29] 董传甫, 林天龙, 陈日升, 等. 日本鳗鲡败血腹水病病原研究[J]. 水产科学, 2002, 21(1): 5-8.
DONG C F, LIN T L, CHEN R S, et al.Studies on pathogencitices and biochemical characteristics of pathogens which caused Anguilla japonica bacterial septicaemia and ascites[J]. Fisheries Science, 2002, 21(1): 5-8.(in Chinese with English abstract)
[30] 贺路, 艾晓辉, 左文功. 牛蛙腹水病病原研究[J]. 淡水渔业, 1995, 25(3): 16-18.
HE L, AI X H, ZHOU W G.Study on etiology of ascites of bullfrog[J]. Freshwater fishery, 1995(3): 16-18. (in Chinese)
[31] 高智玲, 纪雪, 陈萍, 等. 致病性嗜水气单胞菌的分离鉴定及耐药性分析[J]. 中国人兽共患病学报, 2016, 32(4): 400-405.
GAO Z L, JI X, CHEN P, et al.Identification and antimicrobial resistance of pathogenic Aeromonas hydrophila[J]. Chinese Journal of Zoonoses, 2016, 32(4): 400-405.(in Chinese with English abstract)
[32] 单晓枫, 郭伟生, 吴同垒, 等. 框镜鲤维氏气单胞菌溶血素基因片段的克隆与序列分析[J]. 中国兽医杂志, 2011, 47(12): 22-24.
SHAN X F, GUO W S, WU T L, et al.Cloning and sequence analysis of hemolysin gene fragment of Aeromonas veronii[J]. Chinese Journal of Veterinary Medicine, 2011, 47(12): 22-24.(in Chinese)

黄颡鱼溶血性腹水病初探

Preliminary study on hemolytic ascites disease of Pelteobagrus fulvidraco

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	徐民民, 黄莹, 李波, 徐艳, 张帅, 姚岭芸, 王政. 生物炭对小麦根际和根内微生物群落结构的影响[J]. 浙江农业学报, 2021, 33(3): 516-525.
[2]	樊利虹, 郭红瑞, 吴江, 易军, 马晓平, 苟丽萍, 谢跃, 叶刚, 左之才. 牛源皮特不动杆菌对小鼠的致病性分析[J]. 浙江农业学报, 2021, 33(2): 230-238.
[3]	黄莉萍, 安莉丽, 李芳, 杨成年, 李虹, 吕光俊, 向枭, 孙翰昌, 翟旭亮, 朱成科. 中华鳖弗氏柠檬酸杆菌的鉴定及病理组织观察[J]. 浙江农业学报, 2020, 32(7): 1176-1186.
[4]	陈乾丽, 汪汉成, 梁永进, 蔡刘体, 黄宇, 周浩, 李忠, 韩洁. 烤后健康烟叶和霉烂烟叶真菌群落结构分析[J]. 浙江农业学报, 2020, 32(6): 1019-1028.
[5]	袁献宇, 杨龙斌, 何赞赞, 毛天骄, 何长生, 占松鹤, 孙裴, 魏建忠, 李郁. 安徽省猪伪狂犬病毒的分离鉴定及其主要毒力基因分子特征[J]. 浙江农业学报, 2020, 32(1): 43-56.
[6]	易可可, 阴文奇, 周远成, 蒋金蓁, 张白玉, 李中银, 颜其贵. 四株猪伪狂犬病毒株的分离鉴定与主要毒力基因分析[J]. 浙江农业学报, 2019, 31(9): 1429-1436.
[7]	王元红, 鲁智敏, 张学琪, 刘自敏, 胡子慧, 杨侃侃, 刘红梅, 潘玲, 彭开松, 李永东, 王勇. 一株J亚型禽白血病病毒的分离鉴定及其gp85基因的序列分析[J]. 浙江农业学报, 2019, 31(5): 709-715.
[8]	康慧敏, 何嘉乐, 刘晨恺, 周瑶佳, 耿毅, 欧阳萍. 四川地区养殖锦鲤浮肿病毒检测与系统进化分析[J]. 浙江农业学报, 2019, 31(4): 539-544.
[9]	张博伟, 王海静, 宋茂勇, 谢慧君, 张建. 四溴双酚A对土壤无机氮转化的影响及其微生物学机理初探[J]. 浙江农业学报, 2019, 31(4): 639-645.
[10]	白明焕, 耿毅, 邓龙君, 甘维熊, 周剑, 黄小丽, 陈德芳, 欧阳萍, 赵若璇, 谌冰洁. 长薄鳅恶臭假单胞菌的分离鉴定及其感染的病理损伤[J]. 浙江农业学报, 2019, 31(2): 207-215.
[11]	崔一龙, 石芸, 杨达汉, 尹有勤, 薛江东, 霍晓伟, 马德慧. 马源蜡样芽孢杆菌的分离鉴定及毒力基因检测[J]. 浙江农业学报, 2019, 31(2): 216-221.
[12]	倪黎纲, 赵旭庭, 王宵燕, 宋成义, 吴信生, 甘源. 基于高通量测序分析肺炎支原体感染姜曲海猪肺组织miRNA表达谱[J]. 浙江农业学报, 2019, 31(12): 1979-1986.
[13]	徐晓锋, 郭成. 淀粉诱导奶牛乳脂下降后奶牛瘤胃细菌菌群变化[J]. 浙江农业学报, 2019, 31(10): 1591-1598.
[14]	张爱菊, 郝雅宾, 郭爱环, 刘金殿, 练青平, 周志明. 基于高通量测序技术的鱼类环境DNA研究中通用引物的筛选验证[J]. 浙江农业学报, 2019, 31(10): 1615-1623.
[15]	桂国弘, 杨华, 朱江群, 朱建芬, 肖英平, 徐娥. 冷鲜鸡冷藏保存过程中菌群结构变化分析[J]. 浙江农业学报, 2019, 31(1): 47-55.