本氏烟DnaJ-like蛋白在芜菁花叶病毒侵染过程中的作用

doi:10.3969/j.issn.1004-1524.2018.12.10

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (12): 2056-2064.DOI: 10.3969/j.issn.1004-1524.2018.12.10

本氏烟DnaJ-like蛋白在芜菁花叶病毒侵染过程中的作用

孙超尘^1,2, 燕飞², 陈剑平^2,*

1.云南农业大学植物保护学院,云南昆明 650201;
2.浙江省植物有害生物防控国家重点实验室培育基地,农业农村部植保生物技术重点实验室,浙江省植保生物技术重点实验室,浙江省农业科学院病毒学与生物技术研究所,浙江杭州 310021

收稿日期:2018-04-23 出版日期:2018-12-25 发布日期:2018-12-28
通讯作者: 陈剑平,E-mail: jpchen2001@126.com
作者简介:孙超尘(1992—),女,山东泰安人,硕士研究生,主要开展病毒-植物互作研究。E-mail: sunchaochen@126.com
基金资助:
公益性行业(农业)专项(201303028)

Function of DnaJ-like proteins in infection of Turnip mosaic virus onto Nicotiana benthamiana

SUN Chaochen^1,2, YAN Fei², CHEN Jianping^2,*

1. College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China;
2. State Key Laboratory Breeding Base of Zhejiang Sustainable Pest and Disease Control, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Rural Affairs, Key Laboratory of Biotechnology in Plant Protection of Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2018-04-23 Online:2018-12-25 Published:2018-12-28

摘要/Abstract

摘要： 热休克蛋白40(HSP40)因其蛋白家族含有保守的J结构域又被称为DnaJ蛋白,其同源蛋白被称为DnaJ-like蛋白。研究表明,DnaJ蛋白家族响应了多种病毒的侵染,但发挥作用的方式各不相同。芜菁花叶病毒(TuMV)是一类重要的蔬菜病毒,给蔬菜生产造成严重经济损失。DnaJ-like蛋白是否响应TuMV侵染,是否在TuMV侵染过程中发挥作用目前尚不清楚。本实验在本氏烟中克隆了分属DnaJ蛋白A类亚家族和B类亚家族的16条基因;qRT-PCR分析表明,DnaJ-like蛋白家族基因在TuMV侵染后表达上调。分别沉默2个亚家族的典型基因NbJ1和NbJ5后,TuMV在沉默植株上的病毒积累量显著较少。结果表明,DnaJ-like蛋白可能在TuMV侵染过程中发挥作用,这为深入了解TuMV的致病机制提供了新思路。

关键词: 热休克蛋白40, 芜菁花叶病毒, 同源性分析

Abstract: Heat shock protein 40 (HSP40) contains a conserved J domain, also named DnaJ protein. Its homologous protein is called DnaJ-like protein. Current research shows that HSP40 family responds to the infection of multiple viruses, but the ways in their respective functions are different. Turnip mosaic virus (TuMV) is an important vegetable virus that causes serious economic losses to vegetable production. At present, it is unclear whether the DnaJ-like protein responds to the infection of TuMV and whether DnaJ-like protein play a role in TuMV infection. In this study, 16 genes belonging to DnaJ protein class A subfamily and B subfamily were cloned from Nicotiana benthamiana. The qRT-PCR analysis showed that DnaJ-like protein family genes were up-regulated in TuMV-infected N. benthamiana. Silencing the genes NbJ1 and NbJ5, the typical gene of subfamily A and B, respectively, caused the reduced TuMV accumulation. The results showed that DnaJ-like protein might play a role in TuMV infecting, which provided a new way to understand the pathogenic mechanism of TuMV.

Key words: HSP40, turnip mosaic virus, homology analysis

中图分类号:

S432.1

孙超尘, 燕飞, 陈剑平. 本氏烟DnaJ-like蛋白在芜菁花叶病毒侵染过程中的作用[J]. 浙江农业学报, 2018, 30(12): 2056-2064.

SUN Chaochen, YAN Fei, CHEN Jianping. Function of DnaJ-like proteins in infection of Turnip mosaic virus onto Nicotiana benthamiana[J]. , 2018, 30(12): 2056-2064.

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本氏烟DnaJ-like蛋白在芜菁花叶病毒侵染过程中的作用

Function of DnaJ-like proteins in infection of Turnip mosaic virus onto Nicotiana benthamiana

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