基于TaqMan PCR鉴定猪源性成分的引物和探针研发

doi:10.3969/j.issn.1004-1524.2019.01.10

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (1): 74-79.DOI: 10.3969/j.issn.1004-1524.2019.01.10

基于TaqMan PCR鉴定猪源性成分的引物和探针研发

郭梁^1,2,3, 李春冬^1,2, 徐伟良^1,2,3, 郭元晟^1,2,3

1.锡林郭勒职业学院,内蒙古锡林浩特 026000;
2.锡林郭勒生物工程研究院,内蒙古锡林浩特 026000;
3.锡林郭勒食品检验检测和风险评估中心,内蒙古锡林浩特 026000

收稿日期:2018-03-21 出版日期:2019-01-25 发布日期:2019-04-09
作者简介:郭梁(1986—),男,内蒙古锡林浩特人,博士,助理研究员,研究方向为生物工程技术开发。E-mail:herdman86@163.com
基金资助:
锡林郭勒职业学院重点科研课题(ZD2017-10); 锡林郭勒生物工程研究院重点科研课题(2016002)

Research and development of primer and probe for identification of porcine-derived materials inferred from TaqMan PCR

GUO Liang^1,2,3, LI Chundong^1,2, XU Weiliang^1,2,3, GUO Yuansheng^1,2,3

1. Xilingol Vocational College, Xilinhot 026000,China;
2. Xilingol Institute of Bioengineering, Xilinhot 026000,China;
3.Xilingol Food Testing and Risk Assessment Center, Xilinhot 026000,China

Received:2018-03-21 Online:2019-01-25 Published:2019-04-09

摘要/Abstract

摘要： 为了研发具有自主知识产权的猪源性成分检测的引物和探针,比对猪、牛、羊、马、鸡、鸭、兔、鹅等8种动物的线粒体基因组,筛选并设计猪特异性的引物和探针组合进行荧光定量PCR。研究结果表明,所研发的猪源性成分检测的引物和探针仅对猪相关DNA模板有典型扩增曲线,对其他动物来源的DNA模板无扩增,具有高度的猪源性特异性。灵敏度实验表明,利用此引物和探针的检测方法具有高度的灵敏度,可达1 pg级。肉制品中猪源性定量检测实验说明此方法具有较好的定量检测能力。此引物和探针组合适用于猪源性食品和农畜产品的检验检测。

关键词: 猪源性成分, 引物, 探针, TaqMan PCR

Abstract: The objective of this study was to design the primer and probe with intellectual property which can be used to detect porcine-derived materials by means of TaqMan PCR. The results showed that the technique based on the primers and probe was specific for species identification and quantification, and was able to detect 1 pg template. This biotechnological method based on TaqMan PCR was appropriate to identify porcine-derived materials in farm products and foods.

Key words: porcine-derived material, primer, probe, TaqMan PCR

中图分类号:

S828
TS251.1

郭梁, 李春冬, 徐伟良, 郭元晟. 基于TaqMan PCR鉴定猪源性成分的引物和探针研发[J]. 浙江农业学报, 2019, 31(1): 74-79.

GUO Liang, LI Chundong, XU Weiliang, GUO Yuansheng. Research and development of primer and probe for identification of porcine-derived materials inferred from TaqMan PCR[J]. , 2019, 31(1): 74-79.

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基于TaqMan PCR鉴定猪源性成分的引物和探针研发

Research and development of primer and probe for identification of porcine-derived materials inferred from TaqMan PCR

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