浙江省绍兴市茄子黄萎病菌菌株致病型鉴定及其生物学特性研究

doi:10.3969/j.issn.1004-1524.2019.05.14

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (5): 784-789.DOI: 10.3969/j.issn.1004-1524.2019.05.14

浙江省绍兴市茄子黄萎病菌菌株致病型鉴定及其生物学特性研究

李戌清¹, 张敬泽², 张雅¹, 吴根良¹

1.杭州市农业科学研究院,浙江杭州 310024;
2.浙江大学农业与生物技术学院,浙江杭州 310058

收稿日期:2018-10-11 出版日期:2019-05-25 发布日期:2019-05-23
作者简介:李戌清(1982—),女,山西原平人,博士,高级农艺师,主要从事植物病害研究。E-mail: xq_li@126.com
基金资助:
浙江省科技厅重点研发项目(2015C02023);浙江省育种团队项目(2016C02051-2-2);杭州市农业科学研究院重点推广项目(2016HNKT-08)

Identification of pathotype of Verticillium dahlida isolates on eggplant and their biological characteristics in Shaoxing City, Zhejiang Province

LI Xuqing¹, ZHANG Jingze², ZHANG Ya¹, WU Genliang¹

1. Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China;
2. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China

Received:2018-10-11 Online:2019-05-25 Published:2019-05-23

摘要/Abstract

摘要： 用大丽轮枝菌(Verticillium dahlida)落叶和非落叶致病型菌株特异性引物扩增,鉴定了浙江省绍兴市茄子黄萎病菌菌株的致病型,并研究了其生物学特性。特异性引物的聚合酶链式反应扩增结果表明,来自浙江省绍兴市的5个代表性分离菌株(VD-sy1、VD-sy2、VD-sy3、VD-sy4和VD-sy5)均属于落叶型菌株。生物学特性研究结果表明,菌株VD-sy1菌丝生长的最适条件为温度25 ℃,24 h黑暗,有较宽的酸碱度范围(pH 4.0~9.0);在以蔗糖为碳源和以酵母膏为氮源的培养基上菌丝生长较快,线性生长率分别为4.25和4.06 mm·d^-1;在以半乳糖为碳源和以氯化铵为氮源的培养基上生长较慢,线性生长率分别为2.24和2.42 mm·d^-1。

关键词: 茄子黄萎病菌, 致病型, 特异性引物扩增, 生物学特性

Abstract: Based on the method of polymerase chain reaction (PCR) amplification using the specific primers of defoliation and nondefoliation pathotypes of Verticillium dahliae, the pathotype of the isolates causing the verticillium wilt disease of eggplant in Shaoxing City of Zhejiang Province were identified. And the biological characteristics of V. dahliae were also studied. Results of PCR amplification showed that the isolates (VD-sy1, VD-sy2, VD-sy3, VD-sy4 and VD-sy5) obtained all belonged to the defoliating pathotype. The results of biological characteristics showed that VD-sy1 was able to grow at pH between 4.0 to 9.0, and 25 ℃ under 24 h dark condition was beneficial to colony growth; sucrose and yeast extract were the best carbon and nitrogen source for mycelia with linear growth rates of 4.25 and 4.06 mm·d^-1, respectively, while galactose and ammonium chloride showed the least liner growth rates of 2.24 and 2.42 mm·d^-1, respectively.

Key words: Verticillium dahliae, pathotype, specific primer amplification, biological characteristics

中图分类号:

S432.4⁺4

李戌清, 张敬泽, 张雅, 吴根良. 浙江省绍兴市茄子黄萎病菌菌株致病型鉴定及其生物学特性研究[J]. 浙江农业学报, 2019, 31(5): 784-789.

LI Xuqing, ZHANG Jingze, ZHANG Ya, WU Genliang. Identification of pathotype of Verticillium dahlida isolates on eggplant and their biological characteristics in Shaoxing City, Zhejiang Province[J]. , 2019, 31(5): 784-789.

参考文献

[1] 浙江农业大学. 农业植物病理学:下册[M]. 上海: 上海科学技术出版社, 1980: 158.
[2] RODENHISER H A, HURD KARRER A M. Evidence of fusion bodies from urediospore germ tubes of cereal rusts on nutrient-solution agar[J]. Phytopathology, 1947, 37(10): 744-756.
[3] AGRION C N.Plant pathology[M]. 5th edition. USA: Academic Press, 2004: 526-528.
[4] 尤海波, 李景富, 许向阳, 等. 番茄抗黄萎病育种研究进展[J]. 北方园艺, 2003(1): 60-62.
YOU H B, LI J F, XU X Y, et al.Research progress on breeding of tomato resistance to verticillium wilt[J]. Northern Horticulture, 2003(1): 60-62.(in Chinese)
[5] 杨华, 蔡立旺, 潘群斌, 等. 棉花黄萎病研究进展浅述[J]. 江西棉花, 2006, 28(6): 3-6.
YANG H, CAI L W, PAN Q B, et al.Progress of cotton verticillium wilt research[J]. Jiangxi Cotton, 2006, 28(6): 3-6.(in Chinese with English abstract)
[6] 郭岩, 田娟, 王进涛. 茄子黄萎病流行学研究概述[J]. 北方园艺, 1999(5): 32-33.
GUO Y, TIAN J, WANG J T.Epidemiology of verticillium wilt in eggplant[J]. Northern Horticulture, 1999(5): 32-33.(in Chinese)
[7] 华中农业大学. 蔬菜病理学[M]. 北京: 农业出版社, 1989: 54-57.
[8] 张继伟. 茄子黄萎病发生及综合防治技术[J]. 吉林蔬菜, 2009(3): 62-63.
ZHANG J W.Occurrence and integrated control techniques of verticillium wilt in eggplant[J]. Jilin Vegetables, 2009(3): 62-63.(in Chinese)
[9] 董国菊, 贺运春. 晋中市茄子黄萎病为害症状及病原与生物学特性研究[J]. 山西农业大学学报(自然科学版), 2004, 24(3): 237-239.
DONG G J, HE Y C.A study on the disease symptom and the biological characteristics of verticillium wilt of eggplant[J]. Journal of Shanxi Agricultural University(Natural Science Edition), 2004, 24(3): 237-239.(in Chinese with English abstract)
[10] 于淼. 河北省茄子黄萎病菌的分离鉴定及抗性资源筛选[D]. 保定: 河北农业大学, 2013.
YU M.Identification of the pathogens of verticillium wilt on eggplant and screening of resistant germplasm[D]. Baoding: Hebei Agricultural University, 2013. (in Chinese with English abstract)
[11] 张辉. 茄黄萎病的病原生物学特性及其防治[D]. 扬州: 扬州大学, 2003.
ZHANG H.The pathogen biology and control of eggplant verticillium wilt[D]. Yangzhou: Yangzhou University, 2003.(in Chinese with English abstract)
[12] PÉREZ-ARTÉS E, GARCÍA-PEDRAJAS M D, BEJARANO-ALCÁZAR J, et al. Differentiation of cotton-Defoliating and nondefoliating pathotypes of verticillium dahliae by RAPD and specific PCR analyses[J]. European Journal of Plant Pathology, 2000, 106(6): 507-517.
[13] 王莉梅, 石磊岩. 北方棉区棉花黄萎病菌落叶型菌系鉴定[J]. 植物病理学报, 1999, 29(2): 181-189.
WANG L M, SHI L Y.Identification of defoliating strains of Verticillium dahlia from cotton in North China[J]. Acta Phytopathologica Sinica, 1999, 29(2): 181-189. (in Chinese with English abstract)
[14] XIAO Z L, HYDE K D, ZHANG J Z.Synonymy of two species of Bipolaris from aquatic crops of Poaceae[J]. Mycotaxon, 2015, 130(1): 131-143.
[15] LEE S B, TAYLOR J W.Isolation of DNA from fungal mycelia and single spores[M]//INNIS M A, GELFAND D H, SNINSKY J J, et al. PCR protocols. San Diego: Academic Press, 1989: 282-287.
[16] MERCADO-BLANCO J, RODRIGUEZ-JURADO D, PEREZ-ARTES E, et al.Detection of the defoliating pathotype of Verticillium dahlida in infected olive plants by nested PCR[J]. European Journal of Plant Pathology, 2002, 108(1): 1-13.
[17] MERCADO-BLANCO J, RODRÍGUEZ-JURADO D, PÉREZ-ARTÉS E, et al. Detection of the nondefoliating pathotype of Verticillium dahliaein infected olive plants by nested PCR[J]. Plant Pathology, 2001, 50(5): 609-619.
[18] 张武军, 李宁, 罗娜, 等. 茄子黄萎病菌不同致病力菌株生物学特性的比较[J]. 四川农业大学学报, 2006, 24(3): 281-287.
ZHANG W J, LI N, LUO N, et al.Comparison on biological characteristics of different pathogenicity strains of the verticillium wilt pathogen of eggplant[J]. Journal of Sichuan Agricultural University, 2006, 24(3): 281-287.(in Chinese with English abstract)
[19] 张卫, 赵光潜, 刘光珍, 等. 重庆茄子黄萎病菌的初步鉴定[J]. 植物医生, 1997, 10(3): 20-23.
ZHANG W, ZHAO G Q, LIU G Z, et al.Preliminary identification of Verticillium dahliae isolated from eggplant in Chongqing[J]. Plant Doctor, 1997, 10(3): 20-23. (in Chinese)
[20] 陈旭升, 陈永萱, 黄骏麒. 棉花黄萎病菌鉴定技术进展[J]. 棉花学报, 1997, 9(2): 64-67.
CHEN X S, CHEN Y X, HUANG J Q.The developmental trend of verticillium identification technique[J]. Acta Gossypii Sinica, 1997, 9(2): 64-67.(in Chinese with English abstract)
[21] SMITH H C.The morphology of Verticillium albo-atrum, V. dahliae and V. tricorpus[J]. New Zealand Journal of Agricultural Research, 1965, 8(3): 450-478.
[22] 顾振芳, 支月娥, 胡育海, 等. 茄子黄萎病菌的研究及其毒素的生物测定[J]. 上海农学院学报, 1995, 13(3): 218-221.
GU Z F, ZHI Y E, HU Y H, et al.A study of verticillium dahliae Kleb on eggplant and biological determination of its toxin[J]. Journal of Shanghai Agricultural College, 1995, 13(3): 218-221. (in Chinese with English abstract)
[23] 朱建兰, 何玉华. 土壤因子对茄子黄萎病菌侵染和生长的影响[J]. 甘肃农业大学学报, 2000, 35(3): 282-286.
ZHU J L, HE Y H.The soil factors influence on infection and growth of verticillium wilt of eggplant[J]. Journal of Gansu Agricultural University, 2000, 35(3): 282-286. (in Chinese with English abstract)
[24] 董国菊. 茄子黄萎病菌(Verticillium dahliae)生物学特性及在病株体内存在状态的研究[D]. 太谷:山西农业大学, 2006.
DONG G J.Studies on biological character of Verticillium dahliae of eggplant and existed forms in infected plants[D]. Taigu: Shanxi Agricultural University, 2006. (in Chinese with English abstract)
[25] 桥本光司. 茄子黄萎病[J]. 中国蔬菜, 1992(5): 49-50.
HASHIMOTO K.Verticillium wilt of eggplant[J]. China Vegetables, 1992(5): 49-50.(in Chinese)
[26] CICCARESE F, AMENDUNI M, SCHIAVONE D, et al.Effect of Verticillium wilt on the yield of susceptible and “slow-wilting” resistant eggplants in the field[J]. Phytopathologia Mediterranea, 1994, 33(3): 212-216.
[27] 黄奔立, 朱华, 朱凤, 等. 茄子黄萎病的发生及病菌生长影响因子[J]. 植物保护学报, 2004, 31(2): 157-160.
HUANG B L, ZHU H, ZHU F, et al.Occurrence of eggplant wilt and factors affecting growth of Verticillium dahliae[J]. Acta Phytophylacica Sinica, 2004, 31(2): 157-160. (in Chinese with English abstract)

浙江省绍兴市茄子黄萎病菌菌株致病型鉴定及其生物学特性研究

Identification of pathotype of Verticillium dahlida isolates on eggplant and their biological characteristics in Shaoxing City, Zhejiang Province

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