浙江省淳安县中华蜜蜂囊状幼虫病流行病学调查

doi:10.3969/j.issn.1004-1524.2019.03.03

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (3): 365-371.DOI: 10.3969/j.issn.1004-1524.2019.03.03

浙江省淳安县中华蜜蜂囊状幼虫病流行病学调查

董捷¹, 郭蕊¹, 郭海坤², 李君竹³, 方勐鸽⁴, 王德前^1,*

1.浙江省农业科学院畜牧兽医研究所,浙江杭州 310021;
2.浙江省农业科学院农产品质量标准研究所,浙江杭州 310021;
3.浙江省淳安千岛湖好清蜜蜜蜂专业合作社,浙江杭州 311701;
4.浙江省淳安千岛湖方兴中蜂专业合作社,浙江杭州 311701

收稿日期:2018-06-29 出版日期:2019-03-25 发布日期:2019-04-08
通讯作者: *王德前,E-mail: wangdeqian2002@sohu.com
作者简介:董捷(1983—),女,山西临汾人,博士,助理研究员, 主要从事传粉昆虫生理及病害研究。E-mail: smalljie1983@163.com
基金资助:
浙江省农业科学院地方科技合作项目(CA20170001); 浙江省农业科学院扶持学科项目; 浙江省农业科学院院地合作发展建设项目

Epidemiological investigation of Chinese sacbrood bee disease in honeybee (Apis cerana) in Chun'an County of Zhejiang Province, China

DONG Jie¹, GUO Rui¹, GUO Haikun², LI Junzhu³, FANG Mengge⁴, WANG Deqian^1,*

1. Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
2. Institute of Quality and Standard for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3. Hao Qingmi Beekeeping Professional Cooperative of Qiandao Lake, Zhejiang, Hangzhou 311701, China;
4. Fangxing Beekeeping Professional Cooperative of Qiandao Lake, Zhejiang, Hangzhou 311701, China

Received:2018-06-29 Online:2019-03-25 Published:2019-04-08

摘要/Abstract

摘要： 中蜂囊状幼虫病是制约中国中蜂养殖产业发展的主要病害。本研究在建立中蜂囊状幼虫病RT-PCR检测方法的基础上,对浙江省淳安县的中蜂进行中囊病的流行病学调查。结果显示:建立的RT-PCR检测方法对中囊病病毒特异性和重复性好,且灵敏度高,检测限为37 fg·mL^-1。用建立的RT-PCR检测方法检测淳安县17个乡镇共34个中蜂饲养点的307群工蜂样品,有13个乡镇中囊病病毒呈阳性,其中有6个乡镇阳性检出率超过50%。在中蜂饲养密集和有意蜂混养的地方,中囊病病毒阳性率较高。本研究明确了浙江省淳安县中囊病的流行情况,为该病害的预防和控制提供理论指导。

关键词: 中蜂囊状幼虫病, 反转录聚合酶链式反应, 流行病学, 淳安县

Abstract: Chinese sacbrood bee disease is one of the serious diseases of Chinese honeybee, Apis cerana cerana. In this study, a RT-PCR method was developed to detect Chinese sacbrood bee virus (CSBV). Furthermore, an epidemiological investigation of CSBV in Chun'an County of Zhejiang Province was conducted based on the new method. The results showed that the developed RT-PCR method had a good repeatability, a sharp specificity and a high sensibility, whose minimum detecting concentration was 37 fg·mL^-1. The epidemiology of CSBV was investigated from 34 sample sites of 17 townships around Qiandao Lake area. The CSBV detection of 13 townships was positive, in which the infection rate was over 50 % at 6 towns. The high infection rate of CSBV may be related to the high density and mixture honeybee species keeping. In conclusion, the epidemic situation of Chinese sacbrood bee disease in Chun'an County was clarified and provided useful information for the disease control.

Key words: Chinese sacbrood bee disease, reverse transcription-polymerase chain reaction, epidemiology, Chun'an County;

中图分类号:

S895.1⁺33

董捷, 郭蕊, 郭海坤, 李君竹, 方勐鸽, 王德前. 浙江省淳安县中华蜜蜂囊状幼虫病流行病学调查[J]. 浙江农业学报, 2019, 31(3): 365-371.

DONG Jie, GUO Rui, GUO Haikun, LI Junzhu, FANG Mengge, WANG Deqian. Epidemiological investigation of Chinese sacbrood bee disease in honeybee (Apis cerana) in Chun'an County of Zhejiang Province, China[J]. , 2019, 31(3): 365-371.

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浙江省淳安县中华蜜蜂囊状幼虫病流行病学调查

Epidemiological investigation of Chinese sacbrood bee disease in honeybee (Apis cerana) in Chun'an County of Zhejiang Province, China

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