四川地区养殖锦鲤浮肿病毒检测与系统进化分析

doi:10.3969/j.issn.1004-1524.2019.04.05

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (4): 539-544.DOI: 10.3969/j.issn.1004-1524.2019.04.05

四川地区养殖锦鲤浮肿病毒检测与系统进化分析

康慧敏, 何嘉乐, 刘晨恺, 周瑶佳, 耿毅, 欧阳萍^*

四川农业大学动物医学院,四川成都 611130

收稿日期:2018-06-20 出版日期:2019-04-25 发布日期:2019-04-19
通讯作者: *欧阳萍,E-mail: ouyang.ping@live.cn
作者简介:康慧敏(1997-),女,四川乐山人,主要从事水生动物病原学和病理学研究。E-mail:kangkang9704@126.com
基金资助:
中国博士后科学基金(2015M582563);四川省教育厅基础研究项目(17ZB0345)

Detection and phylogenetic analysis of carp edema virus (CEV) in farmed koi carp in Sichuan Province

KANG Huimin, HE Jiale, LIU Chenkai, ZHOU Yaojia, GENG Yi, OUYANG Ping^*

College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China

Received:2018-06-20 Online:2019-04-25 Published:2019-04-19

摘要/Abstract

摘要： 2017年10月,四川省某锦鲤养殖场暴发一种以嗜睡、腹部肿大、鳃肿胀和眼睛凹陷为临床特征的传染病,累计死亡率高达50%。为研究锦鲤发病病因和疾病流行规律,对病鱼解剖,进行病理组织观察、电镜观察、PCR检测和系统进化分析。结果显示,患病锦鲤眼球浑浊,腹部肿大,鳃肿胀严重;组织病理学观察发现病鱼的鳃、肝脏、肾脏和脑组织细胞变性、坏死,出现大量炎性细胞浸润;透射电镜观察,在肾脏组织中发现病毒粒子。巢氏PCR方法检测鲤浮肿病毒(carp edema virus,CEV),出现特异性扩增产物;基于CEV P4a基因进行系统发育分析,此病毒与英国株R083同源性为99%。本研究为鲤浮肿病毒的起源进化、分类、疾病诊断和防控提供重要依据。

关键词: 鲤浮肿病毒病, 鲤浮肿病毒, 病理损伤, 系统进化分析

Abstract: In October 2017, an infectious disease broke out at a koi carp farm in Sichuan Province. It was characterized by drowsiness, abdominal swelling, gill swelling and eye depression. The cumulative mortality rate was 50%. In order to study its etiology and regularity of the disease, dissection, histopathology, electron microscopy and phylogenetic analysis were carried out. Principal symptoms included ocular opacity, abdominal swelling and gill necrosis. The gill, liver, kidney and brain tissues showed obvious degeneration, necrosis and massive inflammatory cell infiltration. Virus particles were found in kidney under transmission electron microscope. The specific amplification products of carp edema virus (CEV) were detected by nested-PCR. Based on CEV P4a gene, phylogenetic analysis showed that the homology between the isolated strain and British strain R083 was 90%. The result in this study was important for virus evolution, classification, viral diagonosis and disease control of carp edema virus.

Key words: carp edema virus disease(CEVD), carp edema virus(CEV), pathological injury, system evolution analysis

中图分类号:

S943

康慧敏, 何嘉乐, 刘晨恺, 周瑶佳, 耿毅, 欧阳萍. 四川地区养殖锦鲤浮肿病毒检测与系统进化分析[J]. 浙江农业学报, 2019, 31(4): 539-544.

KANG Huimin, HE Jiale, LIU Chenkai, ZHOU Yaojia, GENG Yi, OUYANG Ping. Detection and phylogenetic analysis of carp edema virus (CEV) in farmed koi carp in Sichuan Province[J]. , 2019, 31(4): 539-544.

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四川地区养殖锦鲤浮肿病毒检测与系统进化分析

Detection and phylogenetic analysis of carp edema virus (CEV) in farmed koi carp in Sichuan Province

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