地肤通乳散治疗奶牛乳房炎临床疗效及作用靶点预测

doi:10.3969/j.issn.1004-1524.2019.05.08

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (5): 730-736.DOI: 10.3969/j.issn.1004-1524.2019.05.08

地肤通乳散治疗奶牛乳房炎临床疗效及作用靶点预测

白东东, 李新圃, 杨峰, 罗金印, 王旭荣, 李宏胜^*

中国农业科学院兰州畜牧与兽药研究所,农业农村部兽用药物创制重点实验室,甘肃省新兽药工程重点实验室,甘肃兰州 730050

收稿日期:2018-11-03 出版日期:2019-05-25 发布日期:2019-05-23
通讯作者: *李宏胜,E-mail: lihsheng@sina.com
作者简介:白东东(1992—),男,陕西延安人,硕士研究生,主要从事中兽医临床研究。E-mail: baidd163@hotmail.com
基金资助:
国家重点研发计划(2017YFD0502200);甘肃省国际科技合作项目(17YF1WA169);中国农业科学院奶牛疾病研究创新工程(CAAS-ASTIP-2014-LIHPS-03)

Clinical efficacy and mechanism prediction of Difutongrusan in treatment of bovine mastitis

BAI Dongdong, LI Xinpu, YANG Feng, LUO Jinyin, WANG Xurong, LI Hongsheng^*

Key Laboratory of Veterinary Pharmaceutical Discovery, Ministry of Agriculture and Rural Affairs, Key Laboratory of New Animal Drug Project of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou Gansu 730050, China

Received:2018-11-03 Online:2019-05-25 Published:2019-05-23

摘要/Abstract

摘要： 为探讨地肤通乳散治疗奶牛乳房炎的临床效果、抗菌活性及作用机制,先利用微量肉汤法测定地肤通乳散对奶牛乳房炎主要病原菌体外抗菌活性,再对患有乳房炎的奶牛进行治疗,观察治愈率和有效率,最后利用BATMAN、STRING、Cytoscape等数据库及软件对地肤通乳散治疗奶牛乳房炎作用靶点进行分析。结果显示,地肤通乳散对无乳链球菌抑制、杀灭浓度均为1.52 mg·mL^-1;对大肠埃希菌和金黄色葡萄球菌的最小抑制浓度为25 mg·mL^-1,最小杀菌浓度是最小抑制浓度的2倍;对奶牛乳房炎的治愈率为63.16%,有效率可达89.47%。地肤通乳散中共筛选得到21个活性化合物,对应靶点173个,枢纽靶点5个。上述结果说明,地肤通乳散对奶牛乳房炎主要病原菌体外抗菌活性较高,临床疗效确切,可能主要通过枢纽靶点5'-核苷酸酶、白介素-8、雌激素受体1、磷脂酰肌醇-4,5-二磷酸3-激酶催化亚基α和磷脂酰肌醇-4,5-二磷酸3-激酶催化亚基β作用于肿瘤坏死因子、Janus激酶2、Toll样受体4、原癌基因RelA、NF-κB亚基和趋化因子CXCL12来治疗奶牛乳房炎。

关键词: 地肤通乳散, 奶牛乳房炎, 抗菌活性, 临床疗效, 作用机制

Abstract: To study the antibacterial activity, clinical effect and mechanism of Difutongrusan in the treatment of bovine mastitis. The antibacterial activities of Difutongrusan against the main pathogens of bovine mastitis were determined by the method of micro broth first, then the cows with mastitis were treated to observe the cure rate and the effective rate. Finally, BATMAN, STRING, Cytoscape databases and softwares were used to analyze the mechanism of treatment of bovine mastitis by Difutongrusan. The results showed that the MIC/MBC of Difutongrusan on Staphylococcus agalactiae was 1.52 mg·mL^-1, the MIC on Escherichia coli and Staphylococcus aureus was 25 mg·mL^-1, and the MBC was twice as high as the MIC; The clinical cure rate of bovine mastitis was 63.16% and the effective rate was 89.47%. A total of 21 active compounds were selected from Difutongrusan, with 173 corresponding targets and 5 pivotal targets. The results indicated that Difutongrusan could have high antibacterial activity in vitro against the main pathogenic bacteria of bovine mastitis, and the clinical efficacy could be definite, which may be mainly through the pivotal target NT5E, IL8, ESR1, PIK3CA and PIK3CB to treat bovine mastitis.

Key words: Difutongrusan, bovine mastitis, antimicrobial activity, clinical efficacy, mechanism of action

中图分类号:

S858.23

白东东, 李新圃, 杨峰, 罗金印, 王旭荣, 李宏胜. 地肤通乳散治疗奶牛乳房炎临床疗效及作用靶点预测[J]. 浙江农业学报, 2019, 31(5): 730-736.

BAI Dongdong, LI Xinpu, YANG Feng, LUO Jinyin, WANG Xurong, LI Hongsheng. Clinical efficacy and mechanism prediction of Difutongrusan in treatment of bovine mastitis[J]. , 2019, 31(5): 730-736.

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地肤通乳散治疗奶牛乳房炎临床疗效及作用靶点预测

Clinical efficacy and mechanism prediction of Difutongrusan in treatment of bovine mastitis

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