脱氧雪腐镰刀菌烯醇和黄曲霉毒素B1单一及联合染毒对小鼠脑组织病理变化、抗氧化性能和紧密连接蛋白表达量的影响

doi:10.3969/j.issn.1004-1524.2019.06.04

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (6): 886-892.DOI: 10.3969/j.issn.1004-1524.2019.06.04

脱氧雪腐镰刀菌烯醇和黄曲霉毒素B₁单一及联合染毒对小鼠脑组织病理变化、抗氧化性能和紧密连接蛋白表达量的影响

王希春¹, 曹利¹, 刘秦¹, 董妍丽¹, 王中正^{1, 2}, 刘锦芳¹, 朱雷¹, 冯士彬¹, 李玉¹, 吴金节¹

1.安徽农业大学动物科技学院,安徽合肥 230036;
2.安徽省铜陵市农业委员会,安徽铜陵 244000

收稿日期:2018-09-10 出版日期:2019-06-25 发布日期:2019-06-26
作者简介:王希春(1978—),男,安徽利辛人,博士,副教授,研究方向为畜禽营养代谢病与中毒病。E-mail: wangxichun@ahau.edu.cn
基金资助:
安徽省生猪产业技术体系(AHCYJSTX-05)

Effects of single and combined exposure of deoxynivalenol and aflatoxin B₁ on pathological changes, antioxidant capacity and tight junction protein expression of brain tissue in mice

WANG Xichun¹, CAO Li¹, LIU Qin¹, DONG Yanli¹, WANG Zhongzheng^{1, 2}, LIU Jinfang¹, ZHU Lei¹, FENG Shibin¹, LI Yu¹, WU Jinjie¹

1. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China;
2. Anhui Agriculture Committee of Tongling City, Tongling 244000, China

Received:2018-09-10 Online:2019-06-25 Published:2019-06-26

摘要/Abstract

摘要： 本试验旨在研究脱氧雪腐镰刀菌烯醇(DON)和黄曲霉毒素B₁(AFB₁)单一及联合染毒对小鼠脑组织病理变化、抗氧化性能和紧密连接蛋白mRNA表达量的影响。选取96只18日龄昆明系雄性小鼠,随机分为对照组、DON组、AFB₁组和AD组(DON+AFB₁组),每组分别灌服生理盐水、500 μg·kg^-1 DON、200 μg·kg^-1 AFB₁和200 μg·kg^-1 AFB₁+500 μg·kg^-1 DON,连续灌服45 d。分别于试验的第0、15、30、45天,每组随机选取6只小鼠,麻醉后处死,取脑组织观察病理变化,并检测脑组织氧化与抗氧化指标及紧密连接蛋白ZO-1和Occludin mRNA的表达量。结果显示,与对照组相比,DON组小鼠脑组织超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性显著下降(P<0.05),NO含量显著升高(P<0.05);AFB₁组小鼠脑组织中CAT活性显著下降(P<0.05);AD组脑组织总抗氧化能力(T-AOC)、CAT、SOD活性显著下降(P<0.05),丙二醛(MDA)和NO含量显著上升(P<0.05);各染毒组脑组织ZO-1和Occludin mRNA的表达量显著下降(P<0.05)。研究结果表明,DON与AFB₁单一及联合染毒均能造成小鼠脑组织不同程度的病理变化,降低抗氧化性能及紧密连接蛋白ZO-1和Occludin mRNA的表达量,且DON与AFB₁的联合毒性大于单一毒性。

关键词: 脱氧雪腐镰刀菌烯醇, 黄曲霉毒素B₁, 联合染毒, 抗氧化性能, 紧密连接蛋白

Abstract: The present experiment was conducted to evaluate the effects of single and combined exposure of deoxynivalenol (DON) and aflatoxin B₁ (AFB₁) on pathological changes, antioxidant capacity and tight junction protein expression of brain tissues in mice. Ninety-six 18-day-old Kunming(male)mice were randomly divided into control group, DON group, AFB₁ group and AD group (DON+AFB₁ group). Each group was fed with physiological saline, 500 μg·kg^-1 DON, 200 μg·kg^-1 AFB₁ and 200 μg·kg^-1 AFB₁+500 μg·kg^-1 DON by gavage respectively. The trial was conducted for 45 d. On the days 0, 15, 30 and 45 of the experiment, six mice in each group were randomly selected and killed after anesthesia,the brain tissues were collected for observing pathological change and measuring the oxidative and antioxidant indexes and the mRNA expression of tight junction protein ZO-1 and Occludin of brain tissue in mice. The results showed that compared with the control group, the activities of superoxide dismutase (SOD) and catalase (CAT) in brain tissues of DON group were decreased significantly (P<0.05), while the content of NO was increased significantly (P<0.05). The activity of CAT in brain tissue of AFB₁ group decreased significantly (P<0.05). The activities of T-AOC, CAT and SOD of brain tissues in the AD group were decreased significantly (P<0.05). The contents of MDA and NO of brain tissues were increased significantly (P<0.05).The mRNA expression of ZO-1 and Occludin were decreased significantly in treatment group (P<0.05). In conclusion, single and combined exposure of DON and AFB₁ can lead to different degrees of pathological changes, as well as reduce antioxidant capacity in mouse brain tissue, produce oxidative damage, while decrease the mRNA expression of ZO-1 and Occludin. DON and AFB₁ exhibit a significant interaction with synergistic effects.

Key words: deoxynivalenol, aflatoxin B₁, combined toxicity, antioxidant capacity, tight junction protein

中图分类号:

S859

王希春, 曹利, 刘秦, 董妍丽, 王中正, 刘锦芳, 朱雷, 冯士彬, 李玉, 吴金节. 脱氧雪腐镰刀菌烯醇和黄曲霉毒素B₁单一及联合染毒对小鼠脑组织病理变化、抗氧化性能和紧密连接蛋白表达量的影响[J]. 浙江农业学报, 2019, 31(6): 886-892.

WANG Xichun, CAO Li, LIU Qin, DONG Yanli, WANG Zhongzheng, LIU Jinfang, ZHU Lei, FENG Shibin, LI Yu, WU Jinjie. Effects of single and combined exposure of deoxynivalenol and aflatoxin B₁ on pathological changes, antioxidant capacity and tight junction protein expression of brain tissue in mice[J]. , 2019, 31(6): 886-892.

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脱氧雪腐镰刀菌烯醇和黄曲霉毒素B₁单一及联合染毒对小鼠脑组织病理变化、抗氧化性能和紧密连接蛋白表达量的影响

Effects of single and combined exposure of deoxynivalenol and aflatoxin B₁ on pathological changes, antioxidant capacity and tight junction protein expression of brain tissue in mice

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