DON、ZEA联合染毒对体外培养鸡脾脏淋巴细胞分泌IL-2、IL-4、IL-10和IFN-γ的影响

doi:10.3969/j.issn.1004-1524.2018.07.02

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (7): 1110-1115.DOI: 10.3969/j.issn.1004-1524.2018.07.02

DON、ZEA联合染毒对体外培养鸡脾脏淋巴细胞分泌IL-2、IL-4、IL-10和IFN-γ的影响

张卓¹, 辜彦霏¹, 阳莎莎¹, 王亚超², 邓俊良¹, 任志华^{1, *}

1.四川农业大学动物医学院,四川成都 611130;
2.西南科技大学,四川绵阳 621010

收稿日期:2017-09-21 出版日期:2018-07-20 发布日期:2018-08-02
通讯作者: 任志华,E-mail: zhihua_ren@126.com
作者简介:张卓(1993—),男,四川仪陇人,硕士研究生,主要从事动物毒病研究。E-mail: 631335814@qq.com
基金资助:
国家自然科学基金(31402269)

Effects of DON and ZEA on IL-2, IL-4, IL-10 and IFN-γ of chicken spleen lymphocytes cultured in vitro

ZHANG Zhuo¹, GU Yanfei¹, YANG Shasha¹, WANG Yachao², DENG Junliang¹, REN Zhihua^{1, *}

1.College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. Southwest University of Science and Technology, Mianyang 621010, China

Received:2017-09-21 Online:2018-07-20 Published:2018-08-02

摘要/Abstract

摘要： 旨在研究DON、ZEA联合染毒对体外培养鸡脾脏淋巴细胞分泌IL-2、IL-4、IL-10和IFN-γ的影响。DON、ZEA联合染毒剂量为0.012 5 μg·mL^-1和0.006 25 μg·mL^-1、0.050 μg·mL^-1和0.025 μg·mL^-1、0.2 μg·mL^-1和0.1 μg·mL^-1、0.8 μg·mL^-1和0.4 μg·mL^-1,进行DON、ZEA联合染毒48 h对鸡脾脏淋巴细胞上清液IL-2、IL-4、IL-10和IFN-γ含量及其mRNA表达的影响。结果表明,各染毒组细胞上清液IL-10和IFN-γ蛋白含量,细胞内IL-2、IL-4和 IL-10 mRNA水平随毒素剂量的升高而增加 (P<0.01);各染毒组细胞上清液IL-2和IL-4蛋白含量均随毒素浓度的升高而降低(P<0.01);细胞内IFN-γ mRNA表达量均随毒素浓度的升高而先升高后降低(P<0.01)。DON、ZEA联合染毒导致细胞因子IL-2、IL-4、IL-10和IFN-γ分泌失衡,且呈剂量依赖性;DON、ZEA联合染毒鸡脾淋巴细胞上调或下降促炎性细胞因子的水平; Th1和Th2细胞因子平衡失调,因此造成细胞免疫损伤,从而对细胞产生毒性。

关键词: DON, ZEA, 联合染毒, 脾脏淋巴细胞, 细胞因子

Abstract: The purpose of this study was to investigate the effects of DON and ZEA on the secretion of IL-2, IL-4, IL-10 and IFN-γ in cultured chicken spleen lymphocytes in vitro. The exposure doses of DON and ZEA were DON 0.012 5 μg·mL^-1 and ZEA 0.006 25 μg·mL^-1, DON 0.05 μg·mL^-1 and ZEA 0.025 μg·mL^-1, DON 0.2 μg·mL^-1 and ZEA 0.1 μg·mL^-1, DON 0.8 μg·mL^-1 and ZEA 0.4 μg·mL^-1, individually. Then, the effects of different doses of DON and ZEA on the concentrations and mRNA expressions of IL-2, IL-4, IL-10, and IFN-γ of chicken splenic lymphocytes in vitro were investigated. The results were as followed: The concentrations of IL-10 and IFN-γ, and the mRNA expressions of IL-2, IL-4, and IL-10 were increased with the DON and ZEA concentrations increasing (P<0.01). However, the concentrations of IL-2 and IL-4 were decreased with the DON and ZEA concentrations increasing (P<0.01). And the mRNA expressions of IFN-γ were increased at first and then decreased (P<0.01). DON combined with ZEA can affect the secretion of the IL-2, IL-4, IL-10 and IFN-γ with a dose-dependent. DON, ZEA combined with the spleen lymphocytes of the infected chicken to increase or decrease the level of inflammatory cytokines. The imbalance of Th1 and Th2 cytokines can result in cellular immune damage, which can be toxic to cells.

Key words: DON, ZEA, combined exposure, splenic lymphocyte, cytokines

中图分类号:

S859.87

张卓, 辜彦霏, 阳莎莎, 王亚超, 邓俊良, 任志华. DON、ZEA联合染毒对体外培养鸡脾脏淋巴细胞分泌IL-2、IL-4、IL-10和IFN-γ的影响[J]. 浙江农业学报, 2018, 30(7): 1110-1115.

ZHANG Zhuo, GU Yanfei, YANG Shasha, WANG Yachao, DENG Junliang, REN Zhihua. Effects of DON and ZEA on IL-2, IL-4, IL-10 and IFN-γ of chicken spleen lymphocytes cultured in vitro[J]. , 2018, 30(7): 1110-1115.

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DON、ZEA联合染毒对体外培养鸡脾脏淋巴细胞分泌IL-2、IL-4、IL-10和IFN-γ的影响

Effects of DON and ZEA on IL-2, IL-4, IL-10 and IFN-γ of chicken spleen lymphocytes cultured in vitro

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