不同处理条件下金鱼藻净水效果与微生物群落变化

doi:10.3969/j.issn.1004-1524.2020.06.15

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (6): 1070-1081.DOI: 10.3969/j.issn.1004-1524.2020.06.15

不同处理条件下金鱼藻净水效果与微生物群落变化

史传奇, 胡宝忠, 于少鹏^*, 孟博, 杨春雪, 刘嘉, 丁俊男

哈尔滨学院黑龙江省寒区湿地生态与环境研究重点实验室,哈尔滨湿地研究院, 黑龙江哈尔滨 150086

收稿日期:2019-12-27 出版日期:2020-06-25 发布日期:2020-06-24
通讯作者: *于少鹏,E-mail: wetlands1972@126.com
作者简介:史传奇(1986—),男,黑龙江齐齐哈尔人,博士,讲师,从事湿地生物学研究。E-mail: chuanqi0730@163.com
基金资助:
哈尔滨市科技局优秀学科带头人项目(2017RC2017XK015004); 哈尔滨学院青年博士科研启动基金(HUDF2017210); 黑龙江省寒区湿地生态与环境研究重点实验室2019年开放课题(201906)

Water purification effect of Ceratophyllum demersum L. and change of microbial community under different treatments

SHI Chuanqi, HU Baozhong, YU Shaopeng^*, MENG Bo, YANG Chunxue, LIU Jia, DING Junnan

Heilongjiang Province Key Laboratory of Cold Region Wetland Ecology and Environment Research, Harbin Institution of Wetland Research, Harbin University, Harbin 150086, China

Received:2019-12-27 Online:2020-06-25 Published:2020-06-24

摘要/Abstract

摘要： 为研究不同种植密度和曝气条件下,沉水植物金鱼藻对寒区静水湿地水体净化作用和微生物群落变化的影响,从寒区静水湿地水体采集样品,在实验室投加金鱼藻的固定容器中进行曝气处理,测定总氮(total nitrogen, TN)、总磷(total phosphorus, TP)的去除效果;利用高通量测序技术分析不同时间和不同处理间水体微生物群落结构和多样性变化,结合水体理化因子分析微生物群落变化的影响因素。结果表明:结合金鱼藻用量和能耗,金鱼藻种植密度为4.44 g·L^-1,曝气12 h条件下可达到良好的水体净化效果,TN、TP的去除率分别为51.23%、89.27%。微生物样品测序共得到3 283个操作分类单元(operational taxonomic units,OTU),核心OTU数量为107个,对照组中特有OTU数量较多。总体上看,各组微生物群落多样性均未增加,组间差异显著性逐渐降低;优势门为变形菌门、拟杆菌门和放线菌门,投加适量金鱼藻可提高优势门的相对丰度,但曝气处理使变形菌门、拟杆菌门相对丰度降低。不同样品间微生物属水平上存在较大比例的未分类类群,变形菌门多核杆菌属具有较高的相对丰度,投加适量金鱼藻和曝气处理有利于提高其相对丰度。与对TN、TP浓度变化的影响相比,投加金鱼藻和曝气处理对微生物优势类群影响更加显著。

关键词: 金鱼藻, 曝气, 总氮, 总磷, 微生物群落

Abstract: In order to study the purification effect of submerged macrophyte Ceratophyllum demersum on the still water in cold region and its microbial community change under different planting diversity and different aeration treatments, the still water samples in cold region were collected in this experiment. Aeration treatments were operated in fixed-capacity containers with C. demersum in laboratory, and the removal effects of total nitrogen (TN) and total phosphorus (TP) were determined. The high-throughput sequencing technology was used to analyze the microbial community structure and diversity change in different sampling time and treatments. Combined with the physicochemical factors of water samples, the influencing factors of microbial community change were analyzed. The results showed that: considering the C. demersum amount and energy consumption, water purification achieved better effect when the planting density of C. demersum was 4.44 g·L^-1 under the condition of 12 h aeration treatment, the removal rate of TN and TP was 51.23% and 89.27%, respectively. A total of 3 283 operational taxonomic units (OTU) and 107 core OTUs were obtained from the sequencing of microbial samples. Overall, the diversity of microbial community in each group did not increase, and the difference between groups decreased gradually. The dominant phyla were Proteobacteria, Bacteroidetes and Actinobacteria. The relative abundance of the dominant phylum could be increased by adding proper C. demersum, but the relative abundance of Proteobacteria and Bacteroidetes could be reduced by aeration treatment. There were a large proportion of unclassified groups in different samples, and Polynucleobacter in Proteobacteria had a high relative abundance. Adding proper C. demersum and aeration treatment could improve its relative abundance. Compared with the effect of TN and TP concentration changes, adding C. demersum and aeration treatment had more significant effect on microbial dominant groups.

Key words: Ceratophyllum demersum, aeration, total nitrogen, total phosphorus, microbial community

中图分类号:

史传奇, 胡宝忠, 于少鹏, 孟博, 杨春雪, 刘嘉, 丁俊男. 不同处理条件下金鱼藻净水效果与微生物群落变化[J]. 浙江农业学报, 2020, 32(6): 1070-1081.

SHI Chuanqi, HU Baozhong, YU Shaopeng, MENG Bo, YANG Chunxue, LIU Jia, DING Junnan. Water purification effect of Ceratophyllum demersum L. and change of microbial community under different treatments[J]. , 2020, 32(6): 1070-1081.

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不同处理条件下金鱼藻净水效果与微生物群落变化

Water purification effect of Ceratophyllum demersum L. and change of microbial community under different treatments

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