多孢木霉HZ-31菌株侵染对野燕麦生理机制的影响

doi:10.3969/j.issn.1004-1524.2021.03.14

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (3): 490-496.DOI: 10.3969/j.issn.1004-1524.2021.03.14

多孢木霉HZ-31菌株侵染对野燕麦生理机制的影响

朱海霞, 马永强, 咸文荣^*()

青海大学农林科学院,青海大学省部共建三江源生态与高原农牧业国家重点实验室,农业农村部西宁作物有害生物科学观测实验站,青海省农业有害生物综合治理重点实验室,青海西宁810016

收稿日期:2020-06-08 出版日期:2021-04-02 发布日期:2021-03-25
通讯作者: 咸文荣
作者简介:*, 咸文荣,E-mail: xianwr@sina.com
朱海霞(1985—),女,青海西宁人,博士,副研究员,主要从事杂草生物防治研究。E-mail: zhuhaixia0101@163.com
基金资助:
国家重点研发计划(2017YFD0201601);青海省科技厅应用基础研究项目(2019-ZJ-7057)

Effect of Trichoderma polysporum HZ-31 infection on physiological mechanism of Avena fatua L.

ZHU Haixia, MA Yongqiang, XIAN Wenrong^*()

Academy of Agriculture and Forestry Sciences of Qinghai University, State Key Laboratory of Plateau Ecology and Agriculture of Qinghai University, Scientific Observing and Experimental Station of Crop Pest in Xining of Ministry of Agriculture and Rural Affairs, Key Laboratory of Agricultural Integrated Pest Management in Qinghai Province, Xining 810016, China

Received:2020-06-08 Online:2021-04-02 Published:2021-03-25
Contact: XIAN Wenrong

摘要/Abstract

摘要：

选用青海农田常见杂草野燕麦作为靶标杂草,盆栽接种后测定了多孢木霉HZ-31菌株对野燕麦体内各种生理指标的影响,分析了寄主与病原菌相互作用的生理变化机制。结果表明,多孢木霉HZ-31菌株侵染野燕麦后,对植株生理机制防御酶的影响是多方面的,PAL和POD活性增加,以抵御菌株侵染,其余生理防御指标包括MDA含量、CAT、PPO、SOD活性、可溶性蛋白含量和叶绿素含量则受到菌株侵染的破坏,含量减少、活性降低。被抑制的防御酶作用效应大于被促进的防御酶。HZ-31菌株的侵染钝化或抑制了野燕麦体内主要酶类,中断相应的酶促反应,引起植物广泛的代谢变化,包括细胞膜透性改变、干扰光合作用、蛋白质合成、酚类物质代谢等生理方面的变化。

关键词: 多孢木霉, 野燕麦, 侵染, 生理机制

Abstract:

Avena fatua L. as a common weed in the farmland of Qinghai Province, was selected as the target receptor. The effects of Trichoderma polysporum HZ-31 on various physiological indexes of wild oats were determined after potted inoculation, and the physiological mechanism of the interaction between host and pathogen was analyzed. The results showed that there were various effects of Trichoderma polysporum HZ-31 on the defense enzymes of physiological mechanism of Avena fatua L., PAL and POD activity increased to resist the infection of the strain. The other physiological defense indexes including MDA content, CAT activity, PPO activity, SOD activity, soluble protein content and chlorophyll content were destroyed by the infection of the strain. The effect of the inhibited defense enzyme was greater than that of the promoted defense enzyme. The infection of HZ-31 inactivated or inhibited the main enzymes in Avena fatua L., interrupted the corresponding enzymatic reaction, and caused a wide range of metabolic changes in plants, including changes in cell membrane permeability, interference in photosynthesis, protein synthesis, phenol metabolism and other physiological changes.

Key words: Trichoderma polysporum, Avena fatua L., infection, physiological mechanism

中图分类号:

S451.1

朱海霞, 马永强, 咸文荣. 多孢木霉HZ-31菌株侵染对野燕麦生理机制的影响[J]. 浙江农业学报, 2021, 33(3): 490-496.

ZHU Haixia, MA Yongqiang, XIAN Wenrong. Effect of Trichoderma polysporum HZ-31 infection on physiological mechanism of Avena fatua L.[J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 490-496.

图/表 6

图1 野燕麦接种HZ-31后3、5、7 d的生长表现

Fig.1 Growth performance of Avena fatua L. 3, 5 ,7 days after HZ-31 inoculation

图2 接种HZ-31后野燕麦丙二醛(MDA)含量变化分析

Fig.2 Analysis of the changes of MDA content in A. fatua after HZ-31 inoculation

图3 接种HZ-31后野燕麦过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)活性变化分析

Fig.3 Analysis of the changes of CAT, POD and SOD content in A. fatua after HZ-31 inoculation

图4 接种HZ-31后野燕麦多酚氧化酶(PPO)和苯丙氨酸解氨酶(PAL)活性变化分析

Fig.4 Analysis of the changes of PPO and PAL activity in A. fatua after HZ-31 inoculation

图5 接种HZ-31后野燕麦可溶性蛋白含量变化分析

Fig.5 Analysis of the changes of soluble protein content in A. fatua after HZ-31 inoculation

图6 接种HZ-31后野燕麦叶绿素含量变化分析

Fig.6 Analysis of the changes of chlorophyll content in A. fatua after HZ-31 inoculation

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多孢木霉HZ-31菌株侵染对野燕麦生理机制的影响

Effect of Trichoderma polysporum HZ-31 infection on physiological mechanism of Avena fatua L.

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