甜菜夜蛾幼虫龄数和龄期的测定

doi:10.3969/j.issn.1004-1524.2021.06.08

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (6): 1035-1041.DOI: 10.3969/j.issn.1004-1524.2021.06.08

甜菜夜蛾幼虫龄数和龄期的测定

章金明¹(), 周书行¹, 林雅², 刘敏¹, 王丽坤¹, 吕要斌¹^,²^,^*()

1.浙江省农业科学院植物保护与微生物研究所,浙江杭州 310021
2.浙江师范大学化学与生命科学学院,浙江金华 321004

收稿日期:2021-01-18 出版日期:2021-06-25 发布日期:2021-06-25
通讯作者: 吕要斌
作者简介:*吕要斌,E-mail: luybcn@163.com
章金明(1975—),男,浙江新昌人,硕士,副研究员,主要从事蔬菜害虫综合治理和昆虫化学生态学研究。E-mail: zhanginsect@163.com
基金资助:
国家重点研发计划(2016YFD0201007);浙江省“三农六方”科技协作计划(2020SNLF023);平湖市地方科技合作项目(PH20180005)

Determination of larval instars of Spodoptera exigua (Hübner)

ZHANG Jinming¹(), ZHOU Shuxing¹, LIN Ya², LIU Min¹, WANG Likun¹, LYU Yaobin¹^,²^,^*()

1. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China

Received:2021-01-18 Online:2021-06-25 Published:2021-06-25
Contact: LYU Yaobin

摘要/Abstract

摘要：

甜菜夜蛾[Spodoptera exigua(Hübner)]属鳞翅目(Lepidoptera)夜蛾科(Noctuidae),是芦笋、番茄、棉花、萝卜、玉米等植物上的重要害虫。明确该虫幼虫的龄数和龄期,可为其预测预报和科学防治提供依据。对甜菜夜蛾幼虫各龄期的头壳宽度、体长和体重进行测量,并根据频次分布图、戴氏定律、Crosby生长法则和回归分析推断和验证甜菜夜蛾幼虫的虫龄数。结果显示,甜菜夜蛾1~5龄幼虫的头壳宽度范围分别为0.21~0.30、0.40~0.49、0.65~0.75、1.05~1.15、1.65~1.80 mm,频次分布呈现出5个明显的集中区域;1~5龄幼虫的体长范围分别为1.0~3.1、2.5~6.5、4.0~10.0、5.0~15.0、11.0~27.0 mm,各龄期体长出现重叠,频次分布集中区域不明显;1~5龄幼虫的体重范围分别为0.025~0.567、0.200~6.000、1.100~15.200、6.200~65.600、32.900~275.200 mg。结果表明,试验条件下甜菜夜蛾幼虫共5龄,幼虫头壳宽度可作为分龄指标,而幼虫体长和体重在各龄期出现较大程度的重叠,不宜作为分龄指标。

关键词: 甜菜夜蛾, 幼虫龄期, 头壳宽度, 体长, 体重

Abstract:

Spodoptera exigua(Hübner) (Lepidoptera: Noctuidae) is a serious pest of various crops, such as asparagus, tomato, cotton, radish, corn and so on. Its damaging range is very broad in China, therefore, it is necessary to determine the larval instars, which is an important evidence for forecasting and proper control of this pest. The number of larval instars was determined and tested based on frequency distribution analysis, Dyar’s rule and Crosby’s growth rule after measuring three indexes including head capsule width, body length and body weight. It was found that the range of head capsule width from the 1^st to the 5^th instar of S. exigua was 0.21-0.30, 0.40-0.49, 0.65-0.75, 1.05-1.15, 1.65-1.80 mm, respectively, and frequency distribution analysis showed five obvious concentration areas. The range of body length from the 1^st to the 5^th instar of S. exigua was 1.0-3.1, 2.5-6.5, 4.0-10.0, 5.0-15.0, 11.0-27.0 mm, respectively, which exhibited no obvious concentration areas. The range of body weight from the 1^st to the 5^th instar of S. exigua was 0.025-0.567, 0.200-6.000, 1.100-15.200, 6.200-65.600, 32.900-275.200 mg, respectively. These results indicated that there were 5 instars for S. exigua under the experiment conditions, and the head capsule width could be applied to the identification of larval instars rather than the body length and body weight due to the overlap among instars.

Key words: Spodoptera exigua(Hübner), larval instar, head capsule width, body length, body weight

中图分类号:

S433.4

章金明, 周书行, 林雅, 刘敏, 王丽坤, 吕要斌. 甜菜夜蛾幼虫龄数和龄期的测定[J]. 浙江农业学报, 2021, 33(6): 1035-1041.

ZHANG Jinming, ZHOU Shuxing, LIN Ya, LIU Min, WANG Likun, LYU Yaobin. Determination of larval instars of Spodoptera exigua (Hübner)[J]. Acta Agriculturae Zhejiangensis, 2021, 33(6): 1035-1041.

图/表 7

参考文献 24

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虫龄 Instar	样本量 Number of samples	范围 Range/mm	平均值 Mean/mm	Brooks指数 Brooks’ ratio	Crosby指数 Crosby’s ratio
1	50	0.21~0.30	0.253±0.002 e	—	—
2	50	0.40~0.49	0.440±0.003 d	1.739	—
3	50	0.65~0.75	0.697±0.003 c	1.584	-0.089
4	50	1.05~1.15	1.101±0.003 b	1.580	-0.003
5	50	1.65~1.80	1.739±0.004 a	1.579	<0.001

虫龄 Instar	样本量 Number of samples	范围 Range/mm	平均值 Mean/mm	Brooks指数 Brooks’ ratio	Crosby指数 Crosby’s ratio
1	50	0.21~0.30	0.253±0.002 e	—	—
2	50	0.40~0.49	0.440±0.003 d	1.739	—
3	50	0.65~0.75	0.697±0.003 c	1.584	-0.089
4	50	1.05~1.15	1.101±0.003 b	1.580	-0.003
5	50	1.65~1.80	1.739±0.004 a	1.579	<0.001

虫龄 Instar	样本量 Number of samples	范围 Range/mm	平均值 Mean/mm	Brooks指数 Brooks’ ratio	Crosby指数 Crosby’s ratio
1	50	1.0~3.1	1.831±0.069 e	—	—
2	50	2.5~6.5	3.660±0.136 d	1.999	—
3	50	4.0~10.0	7.104±0.213 c	1.941	-0.029
4	50	5.0~15.0	11.036±0.356 b	1.553	-0.200
5	50	11.0~27.0	17.910±0.539 a	1.623	0.045

虫龄 Instar	样本量 Number of samples	范围 Range/mm	平均值 Mean/mm	Brooks指数 Brooks’ ratio	Crosby指数 Crosby’s ratio
1	50	1.0~3.1	1.831±0.069 e	—	—
2	50	2.5~6.5	3.660±0.136 d	1.999	—
3	50	4.0~10.0	7.104±0.213 c	1.941	-0.029
4	50	5.0~15.0	11.036±0.356 b	1.553	-0.200
5	50	11.0~27.0	17.910±0.539 a	1.623	0.045

虫龄 Instar	样本量 Number of samples	范围 Range/mg	平均值 Mean/mg	Brooks指数 Brooks’ ratio	Crosby指数 Crosby’s ratio
1	50	0.025~0.567	0.218±0.023 c	—	—
2	50	0.200~6.000	1.618±0.166 c	7.422	—
3	50	1.100~15.200	8.608±0.562 c	5.320	-0.283
4	50	6.200~65.600	30.610±2.259 b	3.556	-0.332
5	50	32.900~275.200	101.014±8.772 a	3.300	-0.072

甜菜夜蛾幼虫龄数和龄期的测定

Determination of larval instars of Spodoptera exigua (Hübner)

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