六个丝瓜品种对南方根结线虫的抗性

doi:10.3969/j.issn.1004-1524.2022.05.10

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (5): 959-965.DOI: 10.3969/j.issn.1004-1524.2022.05.10

六个丝瓜品种对南方根结线虫的抗性

陆景伟¹(), 陈国康², 周娜¹, 魏捷¹, 胡燕¹, 郑阳¹, 陶伟林¹^,^*()

1.重庆市农业科学院蔬菜花卉研究所,重庆 401329
2.西南大学植物保护学院,重庆 400716

收稿日期:2020-08-05 出版日期:2022-05-25 发布日期:2022-06-06
通讯作者: 陶伟林
作者简介:* 陶伟林,E-mail: 1274283622@qq.com
陆景伟(1981—),男,内蒙古赤峰人,硕士,助理研究员,主要从事蔬菜育种与新品种示范推广。E-mail: 273685441@qq.com
基金资助:
重庆市技术创新与应用发展专项(cstc2019jscx-gksbx0114);重庆市技术创新与应用发展专项(cstc2019jscx-gksbX0120);国家重点研发计划(2017YFD0201100)

Resistance evaluation of six towel gourd varieties to Meloidogyne incognita

LU Jingwei¹(), CHEN Guokang², ZHOU Na¹, WEI Jie¹, HU Yan¹, ZHENG Yang¹, TAO Weilin¹^,^*()

1. Institute of Vegetable and Flower Research, Chongqing Academy of Agricultural Sciences, Chongqing 401329, China
2. College of Plant Protection, Southwest University, Chongqing 400716, China

Received:2020-08-05 Online:2022-05-25 Published:2022-06-06
Contact: TAO Weilin

摘要/Abstract

摘要：

根结线虫病是我国瓜类蔬菜的主要病害,选育抗病品种是有效防控该病的主要措施。结合田间调查与室内检测重复验证,采用病情指数、繁殖系数等多种抗根结线虫评价方法对丝瓜抗性进行分析,以便为瓜类蔬菜根结线虫多指标抗性评价提供科学参考和借鉴,为丝瓜与其他瓜类蔬菜抗根结线虫的材料创新与新品种(含砧木)培育奠定基础。综合评价供试丝瓜品种对南方根结线虫的抗性水平,经形态学特征鉴定,重庆地区丝瓜的病原线虫为南方根结线虫(Meloidogyne incognita);6个丝瓜品种1、2、5、6、32、33号的田间病情指数分别为65.3、12.5、55.3、18.9、32.0、34.3,根际线虫繁殖系数分别为1.28、0.46、1.12、0.57、0.73、0.78,每5 mm根段组织线虫数量分别为34、4、18、7、9、10 条,每100 mm幼嫩根段上早期平均检出根结数分别为12.2、2.1、14.8、0、0、0。2020年田间和温室试验结果与2019年抗性评价结果基本一致;以上4项指标相互印证,趋于一致,能够作为抗南方根结线虫的评价指标;4项指标数据综合评价,判定2号、6号材料为高抗(HR),32号、33号材料为抗性(R),1号、5号材料为感病(S)。

关键词: 丝瓜, 南方根结线虫, 繁殖系数, 抗性评价

Abstract:

Root-knot nematode disease is one of important diseases on melon crops production in China, utilization of resistant materials is regarded as one successful way for the disease control. The purpose of this study was to provide scientific reference for more convincing resistance evaluation of towel gourd varieties even other crops varieties based on multiple indicators, and it will lay a foundation for breeding new root-knot nematode resistant varieties (including rootstocks) of luffa and melon vegetables. The resistance of six towel gourd materials were evaluated based on three indicators from field incidence investigation and laboratory tests from 2019—2020. The main results of the study were as follows: the pathogen identification of morphological characteristics showed the parasitic nematode was Meloidogyne incognita. The disease index (DI)of six towel gourd materials (No.1, No.2, No.5, No.6, No.32, No.33) were 65.3, 12.5, 55.3, 18.9, 32.0 and 34.3, respectively. The reproduction coefficient of M. incognita in the rhizosphere of six materials were correspondingly calculated as 1.28, 0.46, 1.12, 0.57, 0.73 and 0.78. While, the pathogenic nematode numbers in root tissues per 5 mm of six kinds of host were found to be 34, 4, 18, 7, 9 and 10 individuals.The average number of root nodules per 100 mm detected in the young root segments of six kinds of host were 12.2,2.1,14.8,0,0 and 0 individuals.The main results of field tests and artificial inoculation in greenhouse conducted in 2020, were consistent with those of field tests in 2019. The above results could be summarized that the materials No.2 and No.6 were high resistant (HR) to M. incognita, while No.32 and No.33 were resistant materials (R), the materials No.1 and No.5 were susceptible (S) to root-knot nematodes (M. incognita).

Key words: towel gourd, Meloidogyne incognita, reproduction coefficient, resistance evaluation

中图分类号:

S642.4

陆景伟, 陈国康, 周娜, 魏捷, 胡燕, 郑阳, 陶伟林. 六个丝瓜品种对南方根结线虫的抗性[J]. 浙江农业学报, 2022, 34(5): 959-965.

LU Jingwei, CHEN Guokang, ZHOU Na, WEI Jie, HU Yan, ZHENG Yang, TAO Weilin. Resistance evaluation of six towel gourd varieties to Meloidogyne incognita[J]. Acta Agriculturae Zhejiangensis, 2022, 34(5): 959-965.

图/表 6

参考文献 21

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材料编号 Material No.	病情严重度等级的对应株数Diseased plants in different grades						总株数 Total plants	发病率 Diseased rate/%	根结指数(病情指数) Root knot index (Disease index)
材料编号 Material No.	0级 0 grade	1级 1 grade	2级 2 grade	3级 3 grade	4级 4 grade	5级 5 grade	总株数 Total plants	发病率 Diseased rate/%	根结指数(病情指数) Root knot index (Disease index)
1	0	4	3	1	6	5	19	100.0	3.26(65.3)
2	9	4	3	0	0	0	16	43.8	0.63(12.5)
5	1	3	4	4	1	4	17	94.1	2.76(55.3)
6	8	3	7	0	0	0	18	55.6	0.94(18.9)
32	3	7	7	2	0	1	20	85.0	1.60(32.0)
33	1	9	7	3	1	0	21	92.3	1.71(34.3)

材料编号 Material No.	病情严重度等级的对应株数Diseased plants in different grades						总株数 Total plants	发病率 Diseased rate/%	根结指数(病情指数) Root knot index (Disease index)
材料编号 Material No.	0级 0 grade	1级 1 grade	2级 2 grade	3级 3 grade	4级 4 grade	5级 5 grade	总株数 Total plants	发病率 Diseased rate/%	根结指数(病情指数) Root knot index (Disease index)
1	0	4	3	1	6	5	19	100.0	3.26(65.3)
2	9	4	3	0	0	0	16	43.8	0.63(12.5)
5	1	3	4	4	1	4	17	94.1	2.76(55.3)
6	8	3	7	0	0	0	18	55.6	0.94(18.9)
32	3	7	7	2	0	1	20	85.0	1.60(32.0)
33	1	9	7	3	1	0	21	92.3	1.71(34.3)

材料编号 Material No.	繁殖系数Reproduction coefficient
材料编号 Material No.	抽蔓期 Stretching stage	结果期 Friuting stage	种瓜成熟期 Ripe stage of melon	平均值 Average
1	1.36±0.13 a	1.60±0.11 a	0.89±0.05 a	1.28
2	0.23±0.04 b	0.68±0.05 c	0.48±0.02 b	0.46
5	1.21±0.11 a	1.30±0.09 a	0.88±0.04 a	1.12
6	0.34±0.07 b	0.99±0.05 bc	0.38±0.02 b	0.57
32	0.36±0.03 b	1.02±0.06 b	0.82±0.03 a	0.73
33	0.41±0.06 b	1.07±0.06 b	0.87±0.05 a	0.78

材料编号 Material No.	繁殖系数Reproduction coefficient
材料编号 Material No.	抽蔓期 Stretching stage	结果期 Friuting stage	种瓜成熟期 Ripe stage of melon	平均值 Average
1	1.36±0.13 a	1.60±0.11 a	0.89±0.05 a	1.28
2	0.23±0.04 b	0.68±0.05 c	0.48±0.02 b	0.46
5	1.21±0.11 a	1.30±0.09 a	0.88±0.04 a	1.12
6	0.34±0.07 b	0.99±0.05 bc	0.38±0.02 b	0.57
32	0.36±0.03 b	1.02±0.06 b	0.82±0.03 a	0.73
33	0.41±0.06 b	1.07±0.06 b	0.87±0.05 a	0.78

材料编号 Material No.	病情严重度等级的对应株数Diseased plants in different grades						发病率 Diseased rate/%	病情指数 Disease index	抗病等级 Resistance type
材料编号 Material No.	0级 0 grade	1级 1 grade	2级 2 grade	3级 3 grade	4级 4 grade	5级 5 grade	发病率 Diseased rate/%	病情指数 Disease index	抗病等级 Resistance type
1	0	4	8	10	6	2	100.0	56.0	S
2	11	11	7	1	0	0	63.3	18.7	HR
5	3	6	10	7	3	1	86.7	42.7	S
6	15	13	2	0	0	0	50.0	11.3	HR
32	4	11	13	2	0	0	86.7	28.7	R
33	3	10	13	3	1	0	90.0	32.7	R

六个丝瓜品种对南方根结线虫的抗性

Resistance evaluation of six towel gourd varieties to Meloidogyne incognita

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材料编号 Material No.	卵 Egg	幼虫 Juvenile	成虫 Adult	合计 Total
1	11	6	17	34
2	2	0	2	4
5	10	5	3	18
6	0	2	5	7
32	4	0	5	9
33	4	4	2	10

材料编号 Material No.	卵 Egg	幼虫 Juvenile	成虫 Adult	合计 Total
1	11	6	17	34
2	2	0	2	4
5	10	5	3	18
6	0	2	5	7
32	4	0	5	9
33	4	4	2	10