代谢组学解析大厂茶P113叶色变化规律

doi:10.3969/j.issn.1004-1524.2022.10.06

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (10): 2121-2131.DOI: 10.3969/j.issn.1004-1524.2022.10.06

代谢组学解析大厂茶P113叶色变化规律

李芳¹(), 刘霞¹, 黄政², 何应琴¹, 宋勤飞¹, 牛素贞^*()

1.贵州大学茶学院,贵州贵阳 550025
2.贵阳人文科技学院经济与管理学院,贵州贵阳 550025

收稿日期:2021-11-18 出版日期:2022-10-25 发布日期:2022-10-26
通讯作者: 牛素贞
作者简介:*牛素贞,E-mail: niusuzhen@163.com
李芳(1992—),女,穿青人,贵州毕节人,硕士研究生,研究方向为茶树种质资源与遗传改良。E-mail: Funfli@163.com
基金资助:
国家重点研发计划(2021YFD1100307);贵州省自然科学基金重点项目(黔科合基础〔2019〕1404号);国家自然科学基金(32060700);贵州省教育厅重点领域项目(黔教合KY〔2021〕042(黔教合KY〔2021〕042)

Change regularity of leaf color of tea plant P113 (Camellia tachangensis F. C. Zhang) by metabolomics

LI Fang¹(), LIU Xia¹, HUANG Zheng², HE Yingqin¹, SONG Qinfei¹, NIU Suzhen^*()

1. College of Tea Science, Guizhou University, Guiyang 550025, China
2. College of Economics and Management, Guiyang Institute of Humanities and Science, Guiyang 550025, China

Received:2021-11-18 Online:2022-10-25 Published:2022-10-26
Contact: NIU Suzhen

摘要/Abstract

摘要：

为探究大厂茶P113叶色随季节变换的规律,为特异茶树种质资源鉴定与开发奠定研究基础,以茶树新品种大厂茶紫化植株P113为材料,采用三重四级杆质谱多反应监测模式(multiple reaction monitoring,MRM)对其春季、夏季和秋季的一芽二叶新梢进行黄酮类代谢物鉴定。结果显示,共检测到165个黄酮类物质的离子峰,包括正离子峰71个,负离子峰94个,包含花青素(6.06%)、双黄酮(2.42%)、查尔酮(0.6%)、二氢黄酮(4.24%)、二氢黄酮醇(3.03%)、黄烷醇类(9.70%)、黄酮(26.67%)、黄酮碳糖苷(4.24%)、黄酮醇(26.67%)、异黄酮(2.42%)、原花青素(7.88%)和单宁(6.06%)共12种黄酮类物质。主成分分析(PCA)显示,春季、夏季和秋季之间的代谢物含量变异较大,季节内的代谢物含量变异较小,说明黄酮类物质在季节变换过程中发生了显著的动态变化。韦恩分析显示:春季与夏季有158个代谢物共有,夏季与秋季有160个代谢物共有,春季与秋季有157个代谢物共有;春季有2个特有代谢物,为异橙黄酮和3,5,6,7,8,3',4'-七甲氧基黄酮,夏季和秋季均没有特有代谢物,总体上大部分黄酮类代谢物在每一个时期都有表达。相关性分析显示,花青素含量与二氢黄酮醇、黄酮、黄酮碳糖苷、黄酮醇、原花青素类含量呈正相关,与双黄酮、查尔酮、二氢黄酮、黄烷醇类、单宁类含量呈负相关。因此,推测大厂茶P113的叶色紫化与黄酮类物质含量,尤其是花青素含量变化有着密切的关系。

关键词: 代谢组, 大厂茶, 黄酮类物质, 叶色

Abstract:

To explore the change regularity of leaf color of tea plant P113 with the seasons, and lay a research foundation for the identification and development of special tea germplasm resources, tea plant P113(Camellia tachangensis F. C. Zhang) was used as material, and multiple reaction monitoring (MRM) was used to identify the flavonoids metabolites of one bud with two leaves of P113 in spring, summer and autumn. The results showed that 165 ion peaks of flavonoids were detected, including 71 positive ion peaks and 94 negative ion peaks, including anthocyanins (6.06%), biflavones (2.42%), chalcone (0.6%), dihydroflavones (4.24%), dihydroflavonols (3.03%), flavanols (9.70%), flavonoids (26.67%), flavonoid carbonosides (4.24%), flavonols (26.67%) and isoflavones (2.42%), proanthocyanidins (7.88%) and tannins (6.06%). There were 12 kinds of flavonoids in total. Principal component analysis showed that the variation of metabolite contents between spring, summer and autumn were large, and the variation of metabolite content within the season were small, indicating that flavonoids had undergone significant dynamic changes in the process of seasonal change. Venn analysis showed that there were 158 common metabolites in spring and summer, 160 common metabolites in summer and autumn, 157 common metabolites in spring and autumn, and 2 specific metabolites (isoorange flavone and 3,5,6,7,8,3', 4'-heptamethoxyflavone) existed in spring, and there were no specific metabolites in summer and autumn. In general, most flavonoids metabolites were expressed in each period. Correlation analysis showed that anthocyanins were positively correlated with dihydroflavonols, flavonoids, flavone carboglycosides, flavonols and procyanidins, and negatively correlated with biflavones, chalcone, dihydroflavonoids, flavanols and tannins. Therefore, this study suggested that the leaf color purple change of C.tachangensis P113 was closely related to the content of flavonoids, especially anthocyanins.

Key words: metabolome, Camellia tachangensis F.C.Zhang, flavonoids, leaf color

中图分类号:

S571.1

李芳, 刘霞, 黄政, 何应琴, 宋勤飞, 牛素贞. 代谢组学解析大厂茶P113叶色变化规律[J]. 浙江农业学报, 2022, 34(10): 2121-2131.

LI Fang, LIU Xia, HUANG Zheng, HE Yingqin, SONG Qinfei, NIU Suzhen. Change regularity of leaf color of tea plant P113 (Camellia tachangensis F. C. Zhang) by metabolomics[J]. Acta Agriculturae Zhejiangensis, 2022, 34(10): 2121-2131.

图/表 8

图1 P113茶树在春(A)、夏(B)、秋(C)的生长情况

Fig.1 Growth of P113 tea plant in spring (A), summer (B) and autumn (C)

图2 QC样本质谱检测总离子流图(TIC)叠加图

Fig.2 Superposition of total ion current (TIC) of QC samples detected by mass spectrometry

图3 主成分分析得分 A、B、C分别代表春、夏、秋各时期的样本。主成分所占比例越大,代表该主成分区分样本的可信度越大。

Fig.3 Score of principal component analysis A, B and C represented samples in spring, summer and autumn, respectively. The greater the proportion of principal component, the greater the reliability of the sub sample representing the principal component.

图4 代谢物占比

Fig.4 Proportion of metabolite proportion

图5 不同季节样本间的韦恩图 A、B和C代表春季、夏季和秋季。不同颜色的圈表示不同季节的代谢物集合,重叠的圈表示不同季节的相同代谢物,数字表示代谢物数量。

Fig.5 Venn diagram between samples in different seasons A, B and C represented spring, summer and autumn. Circles of different colors represented the collection of metabolites in different seasons, and overlapping circles represented the same metabolites in different seasons, and the number indicated the number of metabolites.

图6 不同季节黄酮类物质的表达量图A中所有柱状图的纵坐标表示峰值归一化后的值(Fold change),横坐标A、B、C分别表示春、夏、秋;图B中纵坐标表示代谢物名称,横坐标表示峰值归一化后的值,不同的颜色表示不同的季节。

Fig.6 Expression of flavonoid in different seasons The ordinate of all histograms in figure A represented the normalized value of the peak (Fold change), and the abscissa A, B and C represented spring, summer and autumn, respectively; In figure B, the ordinate represented the metabolite name, the abscissa represented the peak normalized value, and different colors represented different seasons.

图7 不同类别代谢物之间的相关性热图右侧和下侧为代谢物类别名称,左侧和上侧为样本聚类树,左上角图例是不同r值的颜色区间,颜色越红,表示代谢物间的相关性越大,颜色越蓝,表示代谢物间的相关性越小。

Fig.7 Heat map of correlation between different categories of metabolites The right and lower sides were metabolite category names, the left and upper sides were sample clustering trees, and the legend in the upper left corner was the color range of different r values. The redder the color, the greater the correlation between metabolites, and the bluer the color, the smaller the correlation between metabolites.

图8 黄酮类物质代谢路径图

Fig.8 Metabolic pathway of flavonoids

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代谢组学解析大厂茶P113叶色变化规律

Change regularity of leaf color of tea plant P113 (Camellia tachangensis F. C. Zhang) by metabolomics

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