低温离体保存黄独微型块茎转录组、蛋白质组和代谢组的关联分析

doi:10.3969/j.issn.1004-1524.2017.11.08

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (11): 1827-1834.DOI: 10.3969/j.issn.1004-1524.2017.11.08

低温离体保存黄独微型块茎转录组、蛋白质组和代谢组的关联分析

洪森荣, 吴夏俊鹏, 徐文慧, 占学林, 谢妮妮, 蒋妍, 汪金华, 凌飞, 吴丽霞, 万琳

上饶师范学院生命科学学院,江西上饶 334001

收稿日期:2017-04-05 出版日期:2017-11-20 发布日期:2017-12-05
作者简介:洪森荣(1974—),男,江西永新人,硕士,教授,研究方向为药用植物生物技术。E-mail:hongsenrong@163. com
基金资助:
国家自然科学基金项目(31360072)

Correlation analysis of transcriptome, proteome and metabolome of Dioscorea bulbifera L. microtubers conserved in vitro at low temperature

HONG Senrong, WU-XIA Junpeng, XU Wenhui, ZHAN Xuelin, XIE Ni’ni, JIANG Yan, WANG Jinhua, LING Fei, WU Lixia, WAN Lin

College of Life Sciences, Shangrao Normal University, Shangrao 334001, China

Received:2017-04-05 Online:2017-11-20 Published:2017-12-05

摘要/Abstract

摘要： 为探究黄独微型块茎低温离体保存的内在机理,对其转录组、蛋白质组和代谢组进行关联分析。结果表明:黄独微型块茎低温和常温离体保存的转录本比例和蛋白比例的log₂值均呈现正态分布,十分类似,符合真实生物样品的要求;差异蛋白和差异转录本韦恩图结果表明,84个转录本在两者中均有差异,表明大多数差异的转录本在蛋白组数据均有差异;差异蛋白和差异转录本的log₂对数热图分析结果表明,相对于蛋白质组来说,转录组差异程度更大,两者的差异转录本多数一致,少数不同;差异蛋白和差异转录本log₂散点关联密度图分析结果表明,两者的比例在0附近居多,左下角和右上角(转录本和蛋白的上下调关系一致)相对左上角和右下角(转录和蛋白上下调关系不一致)更多,表明两者差异表达趋势一致性的程度更高。低温离体保存黄独微型块茎转录组、蛋白质组和代谢组的关联分析结果表明,低温离体保存的黄独微型块茎主要涉及碳代谢、氨基酸生物合成、糖酵解途径、淀粉蔗糖代谢和丙酮酸代谢等途径,这为黄独微型块茎的低温离体保存和低温破除休眠提供了理论依据。

关键词: 黄独, 微型块茎, 低温离体保存, 转录组, 蛋白质组, 代谢组

Abstract: In order to explore the internal mechanism of Dioscorea bulbifera L. microtubers conserved in vitro at low temperature, the correlation of transcriptome, proteome and metabolome was analyzed. The results showed that Log₂ of transcript ratio and protein ratio of D. bulbifera L. microtubers conserved in vitro at low temperature and room temperature all showed normal distribution, which were very similar and consistent with the requirements of real biological samples; Wayne plot analysis of differential proteins and differential transcripts showed that the 84 transcripts were different in both groups, indicating that most of the transcripts were differentially expressed in proteome data; Log₂ logarithm thermal analysis of differential proteins and differential transcripts showed that the difference in transcriptome was greater than that in proteome, most of their differential transcripts were consistent, few were different; Analysis of log₂ logarithmic scatter correlation density plot showed that ratio of differential proteins and transcripts was in the vicinity of 0, the lower left corner and upper right corner that indicating the up/down regulation relationship of transcripts and proteins was consistent were more compared with the upper left corner and lower right corner that indicating the up/down regulation relationship of transcripts and proteins was not consistent, which indicated that consistency degree of the trend of differential expression was higher. Correlation analysis results of transcriptome, proteome and metabolome showed that conservation in vitro of D. bulbifera L. microtubers at low temperature mainly involved the pathway, such as carbon metabolism, amino acid biosynthesis, glycolysis, starch and sucrose metabolism, and pyruvate metabolism etc., which could provide a theoretical basis for conservation in vitro at low temperature and low temperature breaking dormancy for D. bulbifera L. microtubers.

Key words: Dioscorea bulbifera L., microtuber, conservation in vitro at low-temperature, transcriptome, proteome, metabolome

中图分类号:

S632.1

洪森荣, 吴夏俊鹏, 徐文慧, 占学林, 谢妮妮, 蒋妍, 汪金华, 凌飞, 吴丽霞, 万琳. 低温离体保存黄独微型块茎转录组、蛋白质组和代谢组的关联分析[J]. 浙江农业学报, 2017, 29(11): 1827-1834.

HONG Senrong, WU-XIA Junpeng, XU Wenhui, ZHAN Xuelin, XIE Ni’ni, JIANG Yan, WANG Jinhua, LING Fei, WU Lixia, WAN Lin. Correlation analysis of transcriptome, proteome and metabolome of Dioscorea bulbifera L. microtubers conserved in vitro at low temperature[J]. , 2017, 29(11): 1827-1834.

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低温离体保存黄独微型块茎转录组、蛋白质组和代谢组的关联分析

Correlation analysis of transcriptome, proteome and metabolome of Dioscorea bulbifera L. microtubers conserved in vitro at low temperature

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