玉米幼苗叶片响应热胁迫的蛋白质组学分析

doi:10.3969/j.issn.1004-1524.2018.06.03

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (6): 893-908.DOI: 10.3969/j.issn.1004-1524.2018.06.03

玉米幼苗叶片响应热胁迫的蛋白质组学分析

石江¹, 赵琳¹, 朱月清², 楼旭平³, 余建忠⁴, 阮松林¹, 陈文岳^{1, *}

1.杭州市农业科学研究院,浙江杭州 310024;
2.杭州市临安区林业局(农业局),浙江杭州 311300;
3.杭州市萧山区农业科学技术研究所,浙江杭州 311202;
4.杭州市淳安县农业局,浙江杭州 311700

收稿日期:2018-02-15 出版日期:2018-06-20 发布日期:2018-07-02
通讯作者: 陈文岳,E-mail: 21572608@qq.com
作者简介:石江(1969—),男,浙江绍兴人,副研究员,主要从事旱粮作物育种与栽培研究。E-mail: 569546851@qq.com

Proteomic analysis of maize seedling leaves in response to heat stress

SHI Jiang¹, ZHAO Lin¹, ZHU Yueqing², LOU Xuping³, YU Jianzhong⁴, RUAN Songlin¹, CHEN Wenyue^{1, *}

1. Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China;
2. Lin'an District Forestry Bureau (Agricultural Bureau) of Hangzhou, Hangzhou 311300, China;
3. Agricultural Science and Technology Institute of Xiaoshan District of Hangzhou City, Hangzhou 311202, China;
4. Agricultural Bureau of Chun'an County of Hangzhou City, Hangzhou 311700, China;

Received:2018-02-15 Online:2018-06-20 Published:2018-07-02

摘要/Abstract

摘要： 玉米是喜温作物,但在生长发育过程中对高温天气敏感,易造成产量下降。以耐热玉米品种先甜5号幼苗为实验材料,在42 ℃下处理0、0.5和3.0 h,提取相应处理的叶片蛋白,进行差异蛋白的筛选,随后对其进行GO分类和KEGG途径分析。结果显示,共筛选到181个差异蛋白,其中上调蛋白95个(定量比值>1.20且P<0.05),下调蛋白86个(定量比值<0.83且P<0.05)。与对照相比,随着热处理时间延长,上调蛋白数明显增加,而下调蛋白数有所下降。GO分类结果显示,2个差异上调蛋白被富集并指定分布在2个细胞组分中,18个差异上调蛋白被富集并指定具有8种分子功能,15个差异上调蛋白被富集并参与17种生物过程。而2个差异下调蛋白被富集并指定分布在8个细胞组分中,29个差异下调蛋白被富集并指定具有11种分子功能,45个差异下调蛋白参与18种生物过程。KEGG分析结果显示,有16个差异上调蛋白被富集并指定参与6个途径,而有14个差异下调蛋白被富集并指定参与8个途径。Domain分析表明,有27个差异上调蛋白被富集并指定含有22个不同结构域,而有34个差异下调蛋白被富集并指定含有21个不同结构域。根据NCBI数据库中信息对上述相关蛋白进行搜索,结果发现假定Dna J分子伴侣蛋白家族、热休克蛋白、硫氧还蛋白H型、脱氢酶和泛素结合酶5类蛋白与耐热关系密切。总之,上述热胁迫响应相关的蛋白质的分析鉴定,可为进一步揭示玉米响应热胁迫的分子机制提供新的思路。

关键词: 玉米, 热响应, 蛋白质组学, GO分类, KEGG路径分析

Abstract: Maize is a warm-tempered crop, but it is sensitive to high temperature weather during its growth and development, which is easy to cause the decline of yield. In this study, seedlings of a heat-resistant maize variety Xiantian No. 5 as experimental materials were treated for 0, 0.5, 3.0 h at 42 ℃, and leaf proteins from all treatments was extracted to carry out screening of differential proteins, and then the GO classification and KEGG pathway were analyzed. The results showed that 181 proteins including 95 up-regulated proteins (quantitative ratio>1.20 and P<0.05) and 86 down-regulated proteins (quantitative ratio<0.83 and P<0.05) were identified. Compared with the control, the number of up-regulated proteins increased with the increase of heat treatment time, while the number of down-regulated proteins decreased. GO classification indicated that in these differentially up-regulation proteins, there were 2 proteins enriched and designated to 2 cell components, 18 proteins enriched and designated to 8 molecular functions and 15 proteins enriched and involved in 17 biological processes. Similarly, in differentially down regulated proteins, there were 2 proteins enriched and distributed in 8 cell components, 29 proteins were enriched and designated to 11 molecular functions. 45 proteins involved in 18 biological processes. KEGG pathway analysis revealed that 16 differentially up-regulated proteins were enriched and designated to participate in 6 pathways, while 14 differentially down-regulated proteins were enriched and designated to participate in 8 pathways. Domain analysis revealed that 27 differentially up-regulated proteins were enriched and designated to 22 various domains, while 34 differentially down-regulated proteins were enriched and designated to 21 different domains. According to the searching information in NCBI database, we found that 5 proteins of Dna J molecular chaperone family including heat shock protein, thioredoxin H type, dehydrogenase and ubiquitin binding enzyme were likely to be closely related to heat resistance. In conclusion, identification of proteins related to heat stress response can provide an insight to further revealing the underlying molecular mechanism of maize response to heat stress.

Key words: maize, heat response, proteomics, GO classification, KEGG pathway analysis

中图分类号:

S513

石江, 赵琳, 朱月清, 楼旭平, 余建忠, 阮松林, 陈文岳. 玉米幼苗叶片响应热胁迫的蛋白质组学分析[J]. 浙江农业学报, 2018, 30(6): 893-908.

SHI Jiang, ZHAO Lin, ZHU Yueqing, LOU Xuping, YU Jianzhong, RUAN Songlin, CHEN Wenyue. Proteomic analysis of maize seedling leaves in response to heat stress[J]. , 2018, 30(6): 893-908.

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玉米幼苗叶片响应热胁迫的蛋白质组学分析

Proteomic analysis of maize seedling leaves in response to heat stress

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