不同环境条件下联合共生固氮菌对小麦种子生长的影响

doi:10.3969/j.issn.1004-1524.2018.06.02

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (6): 886-892.DOI: 10.3969/j.issn.1004-1524.2018.06.02

不同环境条件下联合共生固氮菌对小麦种子生长的影响

邓天天, 陈曦^*, 侯宇梦

河南工程学院,河南郑州 451191

收稿日期:2017-10-10 出版日期:2018-06-20 发布日期:2018-07-02
通讯作者: 陈曦,E-mail:962967834@qq.com
作者简介:邓天天(1987—),女,河南洛阳人,讲师,博士研究生,主要从事农田土壤污染治理工作。E-mail:280233394@qq.com
基金资助:
河南省教育厅项目(16A610016)

Effects of combined symbiotic nitrogen-fixing bacteria on growth of wheat seed under different environmental factors

DENG Tiantian, CHEN Xi^*, HOU Yumeng

Henan Institute of Engineering, Zhengzhou 451191, China

Received:2017-10-10 Online:2018-06-20 Published:2018-07-02

摘要/Abstract

摘要： 从13株小麦根际筛选得到3种联合共生固氮菌,通过单因素批次试验确定了不同菌种的培养时间、温度、pH、葡萄糖浓度及氮素浓度的适宜范围,得出单个菌株和组合菌组生长效果最佳的实验条件,并进一步测定单个菌株和组合菌组对小麦种子生长状况的影响。研究显示,在pH 7,温度35 ℃,NaCl浓度5%,葡萄糖浓度10%,氮素浓度3 g·L^-1的条件下培养36 h时,组合菌组作用于小麦种子的效果优于单个菌株的作用效果,相较于空白组,发芽率平均提高了4%,发芽势、发芽指数也均有所提高。组合菌组能更好地适应多变的环境,稳定发挥作用,有利于获得高产。

关键词: 联合共生固氮菌, 生长曲线, 小麦

Abstract: Three species of co-symbiotic nitrogen fixation bacteria were obtained from 13 strains of wheat. Different strains were determined by the single factor batch experiments to obtain the optimal culture time, temperature, pH, glucose concentration, NaCl concentration and nitrogen concentration; furthermore, to investigate the effects of the single strain and combination groups on the growth of wheat seeds. The results showed that under the conditions of pH 7, the temperature of 35 ℃, NaCl concentration of 5%, glucose concentration of 10%, nitrogen concentration of 3 g·L^-1, culture time of 36 h, the combination of group action showed better effect than the single strain, and compared with the control, the germination rate was increased by 4% on average, the germination potential, germination index also increased. To some extent, the combinatorial bacteria group can better adapt to the changeable environment, playing a stable role in promoting the yield.

Key words: symbiotic nitrogen fixing bacteria, growth curve, wheat

中图分类号:

S512.1

邓天天, 陈曦, 侯宇梦. 不同环境条件下联合共生固氮菌对小麦种子生长的影响[J]. 浙江农业学报, 2018, 30(6): 886-892.

DENG Tiantian, CHEN Xi, HOU Yumeng. Effects of combined symbiotic nitrogen-fixing bacteria on growth of wheat seed under different environmental factors[J]. , 2018, 30(6): 886-892.

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不同环境条件下联合共生固氮菌对小麦种子生长的影响

Effects of combined symbiotic nitrogen-fixing bacteria on growth of wheat seed under different environmental factors

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