一个小麦芒长基因的初步定位及候选基因预测

doi:10.3969/j.issn.1004-1524.20240253

浙江农业学报 ›› 2025, Vol. 37 ›› Issue (1): 14-23.DOI: 10.3969/j.issn.1004-1524.20240253

一个小麦芒长基因的初步定位及候选基因预测

杨晓雨¹^,²(), 马指挥¹^,², 魏青¹^,², 牛志鹏¹^,², 陈安琪¹^,², 胡正冲¹^,², 王林生¹^,²^,^*()

1.河南科技大学农学院,河南洛阳 471023
2.洛阳市作物遗传改良与种质创新重点实验室,河南洛阳 471023

收稿日期:2024-03-18 出版日期:2025-01-25 发布日期:2025-02-14
作者简介:杨晓雨(1999—),女,安徽阜阳人,硕士研究生,主要从事小麦遗传育种研究。E-mail:a18712563532@163.com
通讯作者: *王林生,E-mail:wls282059@haust.edu.cn
基金资助:
河南省农业良种联合攻关项目(2022010104);河南省科技攻关项目(182102110207)

Preliminary mapping of a wheat awn length gene and prediction of candidate genes

YANG Xiaoyu¹^,²(), MA Zhihui¹^,², WEI Qing¹^,², NIU Zhipeng¹^,², CHEN Anqi¹^,², HU Zhengchong¹^,², WANG Linsheng¹^,²^,^*()

1. College of Agriculture, Henan University of Science and Technology, Luoyang 471023, Henan, China
2. Luoyang Key Laboratory of Crop Genetic Improvement and Germplasm Innovation, Luoyang 471023, Henan, China

Received:2024-03-18 Online:2025-01-25 Published:2025-02-14

摘要/Abstract

摘要： 小麦芒作为影响产量和抗逆性的重要农艺性状引起了许多小麦遗传改良工作者的广泛关注。本文以藁优2018(全芒)与科大102(顶芒)及其构建的F₂分离群体为试验材料,在小麦抽穗期对其主要农艺性状进行统计分析,并利用SSR分子标记对小麦芒长基因进行初步定位。结果表明,小麦顶芒对全芒为显性单基因遗传,符合孟德尔分离规律。采用Joinmap4.0软件对芒长基因进行遗传连锁图谱的构建,最终目的基因被初步定位在5A染色体长臂末端的yzu443936和yzu454712两个标记之间。将这两个标记的引物序列与中国春V2.1基因组进行比对,得到两个标记间的物理距离为6.74 Mb ( 668.45~675.19 Mb)。通过IWGSC网站在该区间内共筛选到86个基因,其中有17个基因可能为调控芒长的候选基因。本研究结果可以为小麦芒长基因的精细定位及基因克隆提供理论基础。

关键词: 普通小麦, 芒长, 遗传分析, 候选基因

Abstract:

As an important agronomic trait affecting yield and stress resistance, wheat awn has attracted the attention of many wheat genetic improvement workers. In this paper, the main agronomic traits of Gaoyou 2018 (whole awn), Keda 102 (top awn) and their F₂ isolated populations were statistically analyzed at the heading stage of wheat, and the wheat awn length gene was initially mapped using SSR molecular markers. The results showed that the top and whole awn of wheat were inherited by single dominant gene, which was in accordance with Mendelian separation rule. Joinmap4.0 software was used to construct the genetic linkage map of the awning gene, and the final target gene was initially located between the two markers yzu443936 and yzu454712 at the end of the long arm of chromosome 5A. The primers of these two markers were compared with the genome of Chinese spring V2.1, and the physical distance between the two markers was 6.74 Mb (668.45-675.19 Mb). A total of 86 genes were screened through the IWGSC website, among which 17 genes may be candidate genes for regulating awning length. This study provides a theoretical basis for fine localization and gene cloning of wheat awns.

Key words: common wheat, awn length, genetic analysis, candidate gene

中图分类号:

S512

杨晓雨, 马指挥, 魏青, 牛志鹏, 陈安琪, 胡正冲, 王林生. 一个小麦芒长基因的初步定位及候选基因预测[J]. 浙江农业学报, 2025, 37(1): 14-23.

YANG Xiaoyu, MA Zhihui, WEI Qing, NIU Zhipeng, CHEN Anqi, HU Zhengchong, WANG Linsheng. Preliminary mapping of a wheat awn length gene and prediction of candidate genes[J]. Acta Agriculturae Zhejiangensis, 2025, 37(1): 14-23.

图/表 8

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标记 Marker	染色体 Chr	引物序列 Sequence (5'-3')	退火温度 Tm/℃
yzu443936	5A	F:CCGGCAATACTCACCTCTTT	59
		R:GCTAGCGTTCTGCTGTCCTT
yzu454712	5A	F:TACCACCGATTGATGCTTGA	60
		R:CCGCTAGGGACATATCGAAG
yzu460465	5A	F:AGAGTATTGCGGCCAGCTTA	60
		R:ATCCTACAGCCCCACCCTAT

标记 Marker	染色体 Chr	引物序列 Sequence (5'-3')	退火温度 Tm/℃
yzu443936	5A	F:CCGGCAATACTCACCTCTTT	59
		R:GCTAGCGTTCTGCTGTCCTT
yzu454712	5A	F:TACCACCGATTGATGCTTGA	60
		R:CCGCTAGGGACATATCGAAG
yzu460465	5A	F:AGAGTATTGCGGCCAGCTTA	60
		R:ATCCTACAGCCCCACCCTAT

基因序列号Gene ID	注释信息Annotation	物理位置Physical position/bp
TraesCS5A03G1219200	Post-GPI attachment to proteins factor 3	684 710 738~684 713 456
TraesCS5A03G1219400	—	684 902 731~684 903 105
TraesCS5A03G1219900	Copper transport protein 86	685 124 674~685 127 733
TraesCS5A03G1220200	—	685 156 738~685 159 437
TraesCS5A03G1220800	Probable low-specificity L-threonine aldolase 1	685 405 389~685 408 368
TraesCS5A03G1224200	Peroxisomal membrane protein 11-1	686 771 813~686 774 194
TraesCS5A03G1225300	F-box protein At4g00755	687 299 524~687 304 202
TraesCS5A03G1226300	Defensin-like protein	687 631 077~687 631 292
TraesCS5A03G1227500	RHOMBOID-like protein 2	687 747 729~687 750 510
TraesCS5A03G1230400	Mitochondrial inner membrane protein OXA1	689 537 887~689 543 316
TraesCS5A03G1232600	CASP-like protein 5B3	690 097 794~690 100 067
TraesCS5A03G1235400	Phosphoglucan phosphatase DSP4, amyloplastic	690 618 114~690 623 247
TraesCS5A03G1237100	—	690 817 727~690 820 259
TraesCS5A03G1237500	Major pollen allergen Hol l 1	690 844 500~690 845 663
TraesCS5A03G1238000	Pollen allergen Dac g 3 (Fragment)	691 032 197~691 032 592
TraesCS5A03G1228900	—	689 010 955~689 013 189
TraesCS5A03G1227400	COP9 signalosome complex subunit 1	687 741 228~687 747 570

基因序列号Gene ID	注释信息Annotation	物理位置Physical position/bp
TraesCS5A03G1219200	Post-GPI attachment to proteins factor 3	684 710 738~684 713 456
TraesCS5A03G1219400	—	684 902 731~684 903 105
TraesCS5A03G1219900	Copper transport protein 86	685 124 674~685 127 733
TraesCS5A03G1220200	—	685 156 738~685 159 437
TraesCS5A03G1220800	Probable low-specificity L-threonine aldolase 1	685 405 389~685 408 368
TraesCS5A03G1224200	Peroxisomal membrane protein 11-1	686 771 813~686 774 194
TraesCS5A03G1225300	F-box protein At4g00755	687 299 524~687 304 202
TraesCS5A03G1226300	Defensin-like protein	687 631 077~687 631 292
TraesCS5A03G1227500	RHOMBOID-like protein 2	687 747 729~687 750 510
TraesCS5A03G1230400	Mitochondrial inner membrane protein OXA1	689 537 887~689 543 316
TraesCS5A03G1232600	CASP-like protein 5B3	690 097 794~690 100 067
TraesCS5A03G1235400	Phosphoglucan phosphatase DSP4, amyloplastic	690 618 114~690 623 247
TraesCS5A03G1237100	—	690 817 727~690 820 259
TraesCS5A03G1237500	Major pollen allergen Hol l 1	690 844 500~690 845 663
TraesCS5A03G1238000	Pollen allergen Dac g 3 (Fragment)	691 032 197~691 032 592
TraesCS5A03G1228900	—	689 010 955~689 013 189
TraesCS5A03G1227400	COP9 signalosome complex subunit 1	687 741 228~687 747 570

一个小麦芒长基因的初步定位及候选基因预测

Preliminary mapping of a wheat awn length gene and prediction of candidate genes

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