小麦籽粒类胡萝卜素含量的遗传分析

doi:10.3969/j.issn.1004-1524.2022.10.02

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (10): 2088-2094.DOI: 10.3969/j.issn.1004-1524.2022.10.02

小麦籽粒类胡萝卜素含量的遗传分析

郑文寅¹(), 曾令楠¹, 程颖¹, 侯丞志², 曹文昕³, 赵莉³, 姚大年¹^,^*()

1.安徽农业大学农学院/农业农村部黄淮南片小麦生物学与遗传育种重点实验室,安徽合肥 230036
2.赤峰农牧学校,内蒙古赤峰 024000
3.安徽省农业科学院作物研究所/农作物品质改良安徽省重点实验室,安徽合肥 230001

收稿日期:2021-05-05 出版日期:2022-10-25 发布日期:2022-10-26
通讯作者: 姚大年
作者简介:*姚大年,E-mail: dnyao@163.com
郑文寅(1975—),女,广东汕头人,博士,副教授,从事小麦品质改良和种子科学研究。E-mail: wyzheng@ahau.edu.cn
基金资助:
农作物品质改良安徽省重点实验室开放课题(2020ZW001);国家重点研发计划(2016YFD0300405)

Inheritance of carotenoid content in wheat kernels

ZHENG Wenyin¹(), ZENG Lingnan¹, CHENG Ying¹, HOU Chengzhi², CAO Wenxin³, ZHAO Li³, YAO Danian¹^,^*()

1. College of Agronomy, Anhui Agricultural University/Key Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow & Huai River Valley, Ministry of Agriculture and Rural Affairs, Hefei 230036, China
2. Chifeng Agricuture and Animal Husbandry School, Chifeng 024000, Inner Mongolia, China
3. Institute of Crop Research, Anhui Academy of Agricultural Sciences/Key Laboratory of Crop Quality Improvement of Anhui Province, Hefei 230001, China

Received:2021-05-05 Online:2022-10-25 Published:2022-10-26
Contact: YAO Danian

摘要/Abstract

摘要：

小麦籽粒中类胡萝卜素是一类重要的营养和健康化学成分。本研究利用7个类胡萝卜素含量不同的小麦品种,按Griffing双列杂交法Ⅱ配制21个杂交组合,进行配合力、遗传模型、显隐性基因和遗传参数估算等遗传分析。结果表明,在7个亲本中,生选2号是一个优良的亲本材料,其籽粒类胡萝卜素含量较高,一般配合力较好,可以较快地提高杂交后代类胡萝卜素含量。在亲本选配时应选择高×中类胡萝卜素含量类型。小麦籽粒类胡萝卜素含量为加性-显性-上位性模型遗传,高值受隐性基因控制且遗传力较低。在小麦营养和健康品质育种中,宜在高代进行严格选择以选育出较高类胡萝卜素含量的新品系,达到改良和提高我国小麦营养和健康品质的目的。

关键词: 小麦, 类胡萝卜素含量, Griffing双列杂交Ⅱ, 遗传分析, 营养和健康品质

Abstract:

In order to identify genetics laws of carotenoids in wheat grains, which is the important nutrients and health chemicals, seven wheat varieties with different carotenoid content were used to make 21 cross combinations according to Griffing diallel cross Ⅱ. Furthermore, the combining ability, genetic model, dominant and recessive genes and genetic parameter estimation were analyzed. The results showed that Shengxuan No.2 was an excellent parent material with high carotenoid content in grains and good general combining ability, which could rapidly produce the carotenoid content of hybrids. It is necessary to choose high content parents × medium content parents in parent selection and cross. The inheritance of carotenoid content in wheat grain followed additive-dominant-epistatic model. In addition, the high value of carotenoid was controlled by recessive gene with low heritability. Therefore, strict selection should be carried out in the higher generation to select new lines with higher carotenoids content in wheat nutrition and health quality breeding.

Key words: wheat, carotenoid content, Griffing diallel cross Ⅱ design, inheritance, nutrition and health quality

中图分类号:

S512.1

郑文寅, 曾令楠, 程颖, 侯丞志, 曹文昕, 赵莉, 姚大年. 小麦籽粒类胡萝卜素含量的遗传分析[J]. 浙江农业学报, 2022, 34(10): 2088-2094.

ZHENG Wenyin, ZENG Lingnan, CHENG Ying, HOU Chengzhi, CAO Wenxin, ZHAO Li, YAO Danian. Inheritance of carotenoid content in wheat kernels[J]. Acta Agriculturae Zhejiangensis, 2022, 34(10): 2088-2094.

图/表 9

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亲本Parent	P1	P2	P3	P4	P5	P6	P7
P1	3.622	2.294	3.115	2.576	3.128	3.377	3.336
P2		2.458	2.645	2.614	2.436	2.687	2.648
P3			2.292	2.985	2.656	2.875	3.034
P4				1.985	2.465	2.224	2.254
P5					1.935	1.951	2.052
P6						2.245	2.021
P7							2.328

亲本Parent	P1	P2	P3	P4	P5	P6	P7
P1	3.622	2.294	3.115	2.576	3.128	3.377	3.336
P2		2.458	2.645	2.614	2.436	2.687	2.648
P3			2.292	2.985	2.656	2.875	3.034
P4				1.985	2.465	2.224	2.254
P5					1.935	1.951	2.052
P6						2.245	2.021
P7							2.328

变异来源 Source of variation	自由度 DF	均方 MS	F值 F value	P值 P value
区组Block	2	0.645 7	8.992	0.195 4
基因型Genotype	27	0.121 8	1.683^**	0.000 1
误差Error	54	0.072 4

变异来源 Source of variation	自由度 DF	均方 MS	F值 F value	P值 P value
区组Block	2	0.645 7	8.992	0.195 4
基因型Genotype	27	0.121 8	1.683^**	0.000 1
误差Error	54	0.072 4

变异来源 Source of variation	自由度 DF	均方 MS	F值 F value	P值 P value
一般配合力 General combining ability	6	0.558 7	23.161 8	<0.001
特殊配合力 Special combining ability	21	0.117 1	4.853 4	<0.001
误差Error	54	0.024 1

小麦籽粒类胡萝卜素含量的遗传分析

Inheritance of carotenoid content in wheat kernels

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参考文献 25

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亲本代号 No.	亲本名称 Parent name	效应值 Effect value
P1	苏麦188 Sumai 188	0.499 0 a
P2	镇麦11 Zhenmai 11	-0.046 5 c
P3	生选2号 Shengxuan No.2	0.139 0 b
P4	扬麦21 Yangmai 21	-0.171 9 cd
P5	扬辐麦4号 Yangfumai No.4	-0.232 6 d
P6	扬麦16 Yangmai 16	-0.113 8 cd
P7	扬麦15 Yangmai 15	-0.073 3 c

亲本Parent	P2	P3	P4	P5	P6	P7
P1	-0.735 5	-0.195 6	-0.329 1	0.271 5	0.411 6	0.327 1
P2		-0.033 9	0.255 0	0.128 3	0.262 9	0.183 9
P3			0.444 2	0.168 7	0.270 3	0.389 6
P4				0.292 3	-0.074 4	-0.081 8
P5					-0.278 2	-0.219 5
P6						-0.371 4

参数Parameters	数值 Value
a	-1.991 9
b	0.487 6
t_a₀	8.643 4^**
t_b₀	9.759 2^**
t_b₁	10.256 0^**

参数 Parameters	变异来源 Source of variation	自由度 DF	均方 MS	F值 F value	P值 P value
Wr+Vr	公共亲本间Between common parents	6	5.171 5	11.088 0	0.000 1
	公共亲本内Within common parents	14	0.466 4
Wr-Vr	公共亲本间Between common parents	6	0.717 7	9.084 0	0.000 4
	公共亲本内Within common parents	14	0.079 0

参数Parameter	值Value
加性方差Additive variance	0.098 1
显性方差Dominance variance	0.093 0
遗传方差Hereditary variance	0.191 1
环境方差Environmental variance	0.072 4
表型方差Phenotypic variance	0.214 4
广义遗传力Broad-sense heritability/%	43.36
狭义遗传力Narrow-sense heritability/%	45.78

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