浙江农业学报 ›› 2021, Vol. 33 ›› Issue (3): 497-505.DOI: 10.3969/j.issn.1004-1524.2021.03.15

• 植物保护 • 上一篇    下一篇

酵母双杂交筛选与小麦黄花叶病毒P2互作的寄主因子

韩晓蕾1,2,3, 高仕祺1,2,3, 张帆3, 羊健3, 刘芃3, 姜鸿明1, 李林志1,*()   

  1. 1. 烟台市农业科学研究院,山东 烟台 265500
    2.烟台大学 生命科学学院,山东 烟台 265500
    3.宁波大学/农产品质量安全危害因子与风险防控国家重点实验室,浙江 宁波 315000
  • 收稿日期:2020-10-27 出版日期:2021-04-02 发布日期:2021-03-25
  • 通讯作者: 李林志
  • 作者简介:, 李林志,E-mail: linzhili2002@163.com
    韩晓蕾(1994—),女,黑龙江牡丹江人,硕士,主要从事植物病毒学研究。E-mail: hanxiaolei123@yeah.net
  • 基金资助:
    山东省农业良种工程(2019LZGC001);山东省重大科技创新工程(2018YFJH0602);国家重点研发计划(2017YFD0100600);农业部财政部项目(CARS-3-2-23);丘陵旱地小麦-玉米抗旱减灾技术集成与示范

Screening of host factors interacting with wheat yellow mosaic virus P2 by yeast two-hybrid system

HAN Xiaolei1,2,3, GAO Shiqi1,2,3, ZHANG Fan3, YANG Jian3, LIU Peng3, JIANG Hongming1, LI Linzhi1,*()   

  1. 1. Yantai Academy of Agricultural Sciences, Yantai 265500, China
    2. College of Life Sciences, Yantai University, Yantai 265500, China
    3. Ningbo University/State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ningbo 315000, China
  • Received:2020-10-27 Online:2021-04-02 Published:2021-03-25
  • Contact: LI Linzhi

摘要:

小麦黄花叶病毒(wheat yellow mosaic virus,WYMV)隶属马铃薯Y病毒科(Potyviridae),大麦黄花叶病毒属(Bymovirus)。该病毒基因组是由两条正义单链RNA1和RNA2构成,共编码10个蛋白,其编码的P2在病毒的复制过程中发挥重要功能。文章构建了小麦cDNA酵母文库,通过共转试验共筛选获得若干个候选互作寄主因子,包括水杨酸信号通路因子、E3泛素化连接酶、防御反应、氧化还原酶活性、光系统II组件等18种蛋白,研究P2与寄主因子的互作。进一步试验证实,其中的TaTIFY 10A、Ta14-3-3与WYMV-P2存在互作关系。结果表明,WYMV-P2与小麦的E3泛素化类转录因子、水杨酸信号转录因子、植物光系统的稳定组件及叶绿体的形成关键因子等可能存在互作关系,P2可能参与了寄主多种信号途径,为明确WYMV与寄主的互作机制提供了研究基础。

关键词: 小麦黄花叶病毒, 小麦, P2, cDNA文库, 酵母双杂交

Abstract:

Wheat yellow mosaic virus (WYMV) is classified in the genus Bymovirus, family Potyviridae. The genome is composed of two sense RNA single-stranded RNA1 and RNA2 and encodes 10 proteins, among which P2 plays an important role in virus replication. In order to identify the targets of P2 in host, co-transformation of cDNA library vector and pGBKT7-P2 was constructed, eighteen candidate host targets of P2 were screened out, which were divided into several types based on their functions, including salicylic acid signal pathway factor, E3 ubiquitin ligase, defense response, oxidoreductase activity and photosystem II assembly. Additionally, the interaction of P2 and TaTIFY or Ta14-3-3 was further verified by Y2H system. Based on the results, it could be speculated that P2 may interact with E3 ubiquitin transcription factor, salicylic acid signal transcription factor, or the factors associated with plant photosystem and chloroplasts formation. In conclusion, P2 may participate in multiple signaling pathways during viral infection, and provide an insight into molecular interaction mechanism between WYMV and the host.

Key words: wheat yellow mosaic virus, wheat, P2, cDNA library, yeast two-hybrid

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