外源增钾缓解铵胁迫下小麦根系受抑

doi:10.3969/j.issn.1004-1524.2020.11.01

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (11): 1923-1933.DOI: 10.3969/j.issn.1004-1524.2020.11.01

外源增钾缓解铵胁迫下小麦根系受抑

王峰¹, 叶静¹, 高敬文¹, 王强¹, 俞巧钢¹, 何新华², 马军伟^1,*

1.浙江省农业科学院环境资源与土壤肥料研究所,浙江杭州 310021;
2.西南大学资源与环境学院,重庆 400715

收稿日期:2020-04-22 出版日期:2020-11-25 发布日期:2020-12-02
通讯作者: * 马军伟,E-mail: majw@zaas.ac.cn
作者简介:王峰(1988—),男,江苏徐州人,博士,助理研究员,主要从事植物营养和肥料研究。E-mail: wangfeng@zaas.ac.cn
基金资助:
浙江省重点研发计划(2015C02013,2020R20A50B01); 浙江省农业科学院青年人才培养项目(10102000320CC3001G/003/029)

Potassium alleviates inhibition of ammonium stress on wheat root

WANG Feng¹, YE Jing¹, GAO Jingwen¹, WANG Qiang¹, YU Qiaogang¹, HE Xinhua², MA Junwei^1,*

1. Institute of Environmental Resources and Soil Fertilizers, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
2. College of Resources and Environment, Southwest University, Chongqing 400715, China

Received:2020-04-22 Online:2020-11-25 Published:2020-12-02

摘要/Abstract

摘要： 分别对硝态氮( ${NO}_{3}^{-} - N$ )和铵态氮( ${NH}_{4}^{+} - N$ )培养下的小麦幼苗进行不同水平的钾处理(低钾,1 mmol·L^-1;正常,3 mmol·L^-1;高钾,6 mmol·L^-1),探究外源供钾对缓解铵胁迫下小麦根系生长受抑的效果与作用机理。结果表明, ${NH}_{4}^{+} - N$ 条件下,小麦叶片和根系中的 ${NH}_{4}^{+}$ 含量较 ${NO}_{3}^{-} - N$ 条件下显著(P<0.05)增加,根系生长受到抑制,与 ${NO}_{3}^{-} - N$ 条件下的植株相比, ${NH}_{4}^{+} - N$ 条件下相同钾水平的小麦幼苗总根长、根表面积、根体积均显著(P<0.05)减少。随着施钾水平的升高,根系受抑制的情况得到缓解。 ${NH}_{4}^{+} - N$ 条件下,随着施钾水平的升高,小麦幼苗的叶面积、气孔导度、净光合速率显著(P<0.05)升高,叶和根中的可溶性糖含量显著(P<0.05)升高,叶中蔗糖磷酸合成酶(SPS)活性亦显著(P<0.05)增强,根中生长素(IAA)含量及其与细胞分裂素(CTK)的比值升高。据此推断,在铵胁迫下,增钾处理增强了小麦的光合作用,提高了碳水化合物的合成能力,可为 ${NH}_{4}^{+}$ 的同化提供更多的碳架,从而降低体内 ${NH}_{4}^{+}$ 的积累;同时,促进了根中植物激素的平衡,最终得以缓解铵胁迫下小麦根系生长受到的抑制。

关键词: 小麦, 铵胁迫, 钾, 植物激素, 碳转运

Abstract: To explore the effects of potassium on ammonium stress, wheat seedlings cultivated with nitrate ( ${NO}_{3}^{-} - N$ ) or ammonium ( ${NH}_{4}^{+} - N$ ) nitrogen were treated with different potassium levels (low, 1 mmol·L^-1; normal, 3 mmol·L^-1; high, 6 mmol·L^-1). It was shown that compared with ${NO}_{3}^{-} - N$ , ${NH}_{4}^{+} - N$ significantly (P<0.05) increased ${NH}_{4}^{+}$ concentration in the plants (including root and leaf), and root growth was inhibited as the total root length, root surface area and root volume were significantly (P<0.05) reduced under the same potassium level. With the increasing K dose, the root inhibition was relieved. Under ${NH}_{4}^{+} - N$ conditions, leaf area, stomatal conductance, and net photosynthetic rate were increased with the increasing K dose, which resulted in the significantly (P<0.05) increased soluble sugar content in leaf and root, and thus could provide more carbon skeleton for ${NH}_{4}^{+}$ assimilation. Meanwhile, higher potassium siginificantly (P<0.05) increased the activity of sucrose phosphate synthetase (SPS) activity in leaves. In addition, the indole-3-acetic (IAA) concentration and the ratio of IAA to cytokinin (CTK) in root increased with the increasing K dose. It was inferred that, under ${NH}_{4}^{+} - N$ condition, the exogenous application of potassium enhanced the capability of wheat photosynthesis, provided more carbon skeleton for ${NH}_{4}^{+}$ assimilation, promoted the hormone balance in roots, thus reduced the accumulation of ${NH}_{4}^{+}$ in plant and alleviated root inhibition.

Key words: wheat, ammonium stress, potassium, phytohormone, carbon transport

中图分类号:

S311

王峰, 叶静, 高敬文, 王强, 俞巧钢, 何新华, 马军伟. 外源增钾缓解铵胁迫下小麦根系受抑[J]. 浙江农业学报, 2020, 32(11): 1923-1933.

WANG Feng, YE Jing, GAO Jingwen, WANG Qiang, YU Qiaogang, HE Xinhua, MA Junwei. Potassium alleviates inhibition of ammonium stress on wheat root[J]. , 2020, 32(11): 1923-1933.

参考文献

[1] PIWPUAN N, ZHAI X, BRIX H.Nitrogen nutrition of Cyperus laevigatus and Phormium tenax: effects of ammonium versus nitrate on growth, nitrate reductase activity and N uptake kinetics[J]. Aquatic Botany, 2013, 106: 42-51.
[2] XU G H, FAN X R, MILLER A J.Plant nitrogen assimilation and use efficiency[J]. Annual Review of Plant Biology, 2012, 63(1): 153-182.
[3] BRITTO D T, KRONZUCKER H J.Ecological significance and complexity of N-source preference in plants[J]. Annals of Botany, 2013, 112(6): 957-963.
[4] BRITTO D T, KRONZUCKER H J.

{NH}_{4}^{+}

toxicity in higher plants: a critical review[J]. Journal of Plant Physiology, 2002, 159(6): 567-584.
[5] LI B H, LI G J, KRONZUCKER H J, et al.Ammonium stress in Arabidopsis: signaling, genetic loci, and physiological targets[J]. Trends in Plant Science, 2014, 19(2): 107-114.
[6] WANG F, GAO J, SHI S, et al.Impaired electron transfer accounts for the photosynthesis inhibition in wheat seedlings (Triticum aestivum L.) subjected to ammonium stress[J]. Physiologia Plantarum, 2019, 167(2): 159-172.
[7] ARIZ I, ASENSIO A C, ZAMARREÑO A M, et al. Changes in the C/N balance caused by increasing external ammonium concentrations are driven by carbon and energy availabilities during ammonium nutrition in pea plants: the key roles of asparagine synthetase and anaplerotic enzymes[J]. Physiologia Plantarum, 2013, 148(4): 522-537.
[8] WANG F, GAO J W, LIU Y, et al.Higher ammonium transamination capacity can alleviate glutamate inhibition on winter wheat (Triticum aestivum L.) root growth under high ammonium stress[J]. PLoS One, 2016, 11(8): e0160997.
[9] IKEDA M, KUSANO T, KOGA N.Carbon skeletons for amide synthesis during ammonium nutrition in tomato and wheat roots[J]. Soil Science and Plant Nutrition, 2004, 50(1): 141-147.
[10] CHEN G, GUO S W, KRONZUCKER H J, et al.Nitrogen use efficiency (NUE) in rice links to

{NH}_{4}^{+}

toxicity and futile

{NH}_{4}^{+}

cycling in roots[J]. Plant and Soil, 2013, 369(1/2): 351-363.
[11] TAKEI K, SAKAKIBARA H, TANIGUCHI M, et al.Nitrogen-dependent accumulation of cytokinins in root and the translocation to leaf implication of cytokinin species that induces gene expression of maize response regulator[J]. Plant and Cell Physiology, 2001, 42(1): 85-93.
[12] SZCZERBA M W, BRITTO D T, BALKOS K D, et al.Alleviation of rapid, futile ammonium cycling at the plasma membrane by potassium reveals K+-sensitive and-insensitive components of

{NH}_{4}^{+}

transport[J]. Journal of Experimental Botany, 2008, 59(2): 303-313.
[13] YOUSRA M, AKHTAR J, SAQIB Z A, et al.Effect of potassium application on ammonium nutrition in maize (Zea mays L.) under salt stress[J]. Pakistan Journal of Agricultural Sciences, 2013, 50(1): 43-48.
[14] SPALDING E P, HIRSCH R E, LEWIS D R, et al.Potassium uptake supporting plant growth in the absence of AKT1 channel activity: inhibition by ammonium and stimulation by sodium[J]. The Journal of General Physiology, 1999, 113(6): 909-918.
[15] DREYER I, GOMEZ-PORRAS J L, RIEDELSBERGER J. The potassium battery: a mobile energy source for transport processes in plant vascular tissues[J]. New Phytologist, 2017, 216(4): 1049-1053.
[16] TRÄNKNER M, TAVAKOL E, JÁKLI B. Functioning of potassium and magnesium in photosynthesis, photosynthate translocation and photoprotection[J]. Physiologia Plantarum, 2018, 163(3): 414-431.
[17] BALKOS K D, BRITTO D T, KRONZUCKER H J.Optimization of ammonium acquisition and metabolism by potassium in rice (Oryza sativa L. cv. IR-72)[J]. Plant, Cell & Environment, 2010, 33(1): 23-34.
[18] KRUGLOV S V, SUSLINA L G, ANISIMOV V S, et al., Mechanism of the effect of K+ and

{NH}_{4}^{+}

ions on ¹³⁷Cs accumulation by two-week-old barley plants from soddy-podzolic soils[J]. Pochvovedenie, 2005(10): 1222-1231.
[19] SHIEH R C, LEE Y L.Ammonium ions induce inactivation of Kir2.1 potassium channels expressed in Xenopus oocytes[J]. The Journal of Physiology, 2001, 535(2): 359-370.
[20] CHENG C L, ACEDO G N, CRISTINSIN M, et al.Sucrose mimics the light induction of Arabidopsis nitrate reductase gene transcription[J]. Proceedings of the National Academy of Sciences of the United States of America, 1992, 89(5): 1861-1864.
[21] 於新建. 植物生理学实验手册[M]. 上海: 上海科学技术出版社, 1985.
[22] FUERTES-MENDIZABAL T, GONZALEZ A, MA APARICIO-TEJO J, et al. High irradiance improves ammonium tolerance in wheat plants by increasing N assimilation[J]. Journal of Plant Physiology, 2013, 170: 758-771.
[23] LAUTER F R, NINNEMANN O, BUCHER M, et al.Preferential expression of an ammonium transporter and of two putative nitrate transporters in root hairs of tomato[J]. Proceedings of the National Academy of Sciences of the United States of America, 1996, 93(15): 8139-8144.
[24] SILBER A, BRUNER M, KENIG E, et al.High irrigation frequency and transient

{NH}_{4}^{+}

concentration: effects on soilless-grown bell pepper[J]. The Journal of Horticultural Science and Biotechnology, 2005, 80(2): 233-239.
[25] 李青, 李保海, 施卫明. 高铵胁迫对拟南芥幼苗侧根生长的影响及机制探索[J]. 土壤, 2011, 43(3): 374-381.
LI Q, LI B H, SHI W M.Response and mechanism of Arabidopsis lateral root to excessive ammonium stress[J]. 2011, 43(3): 374-381.(in Chinese with English abstract)
[26] 李保海, 施卫明. 拟南芥幼苗对高

{NH}_{4}^{+}

响应的特征及不同生态型间的差异[J]. 土壤学报, 2007, 44(3): 508-515.
LI B H, SHI W M.Effects of elevated

{NH}_{4}^{+}

on Arabidopsis seedlings difference in accessions[J]. Acta Pedologica Sinica, 2007, 44(3): 508-515.(in Chinese with English abstract)
[27] LI Q, LI B H, KRONZUCKER H J, et al.Root growth inhibition by

{NH}_{4}^{+}

in Arabidopsis is mediated by the root tip and is linked to

{NH}_{4}^{+}

efflux and GMPase activity[J]. Plant, Cell & Environment, 2010, 33(9): 1529-1542.
[28] SETIÉN I, FUERTES-MENDIZABAL T, GONZÁLEZ A, et al. High irradiance improves ammonium tolerance in wheat plants by increasing N assimilation[J]. Journal of Plant Physiology, 2013, 170(8): 758-771.
[29] SZCZERBA M W, BRITTO D T, ALI S A, et al.

{NH}_{4}^{+}

-stimulated and-inhibited components of K+ transport in rice (Oryza sativa L.)[J]. Journal of Experimental Botany, 2008, 59(12): 3415-3423.
[30] MAATHUIS F J M, SANDERS D. Contrasting roles in ion transport of two K+-channel types in root cells of Arabidopsis thaliana[J]. Planta, 1995, 197(3): 456-464.
[31] EL OMARI R, RUEDA-LÓPEZ M, AVILA C, et al. Ammonium tolerance and the regulation of two cytosolic glutamine synthetases in the roots of Sorghum[J]. Functional Plant Biology, 2010, 37(1): 55.
[32] BERNARD S M, MØLLER A L B, DIONISIO G, et al. Gene expression, cellular localisation and function of glutamine synthetase isozymes in wheat (Triticum aestivum L.)[J]. Plant Molecular Biology, 2008, 67(1/2): 89-105.
[33] MARTÍNEZ-ANDúJAR C, GHANEM M E, ALBACETE A, et al. Response to nitrate/ammonium nutrition of tomato (Solanum lycopersicum L.) plants overexpressing a prokaryotic

{NH}_{4}^{+}

-dependent asparagine synthetase[J]. Journal of Plant Physiology, 2013, 170(7): 676-687.
[34] LI B H, SHI W M, SU Y H.The differing responses of two Arabidopsis ecotypes to ammonium are modulated by the photoperiod regime[J]. Acta Physiologiae Plantarum, 2011, 33(2): 325-334.
[35] BECKER T W, CARRAYOL E, HIREL B.Glutamine synthetase and glutamate dehydrogenase isoforms in maize leaves: localization, relative proportion and their role in ammonium assimilation or nitrogen transport[J]. Planta, 2000, 211(6): 800-806.
[36] GUO S, ZHOU Y, SHEN Q, et al.Effect of ammonium and nitrate nutrition on some physiological processes in higher plants-growth, photosynthesis, photorespiration, and water relations[J]. Plant Biology, 2007, 9(1): 21-29.
[37] ERDEI L, HORVÁTH F, TARI I, et al. Differences in photorespiration, glutamine synthetase and polyamines between fragmented and closed stands of Phragmites australis[J]. Aquatic Botany, 2001, 69(2/3/4): 165-176.
[38] PELTIER G, THIBAULT P.Ammonia exchange and photorespiration in Chlamydomonas[J]. Plant Physiology, 1983, 71(4): 888-892.
[39] OLIVEIRA I C, BREARS T, KNIGHT T J, et al.Overexpression of cytosolic glutamine synthetase. relation to nitrogen, light, and photorespiration[J]. Plant Physiology, 2002, 129(3): 1170-1180.
[40] BLOOM A J, SMART D R, NGUYEN D T, et al.Nitrogen assimilation and growth of wheat under elevated carbon dioxide[J]. Proceedings of the National Academy of Sciences of the United States of America, 2002, 99(3): 1730-1735.
[41] MAGALHÃES J R, HUBER D M, TSAI C Y. Evidence of increased ¹⁵N-ammonium assimilation in tomato plants with exogenous α-ketoglutarate[J]. Plant Science, 1992, 85(2): 135-141.
[42] CAO S F, YANG Z F, ZHENG Y H.Sugar metabolism in relation to chilling tolerance of loquat fruit[J]. Food Chemistry, 2013, 136(1): 139-143.
[43] DAS P K, SHIN D H, CHOI S B, et al.Sugar-hormone cross-talk in anthocyanin biosynthesis[J]. Molecules and Cells, 2012, 34(6): 501-507.
[44] GARAY-ARROYO A, DE LA PAZ SÁNCHEZ M, GARCÍA-PONCE B, et al. Hormone symphony during root growth and development[J]. Developmental Dynamics, 2012, 241(12): 1867-1885.
[45] YAMADA M, SAWA S.The roles of peptide hormones during plant root development[J]. Current Opinion in Plant Biology, 2013, 16(1): 56-61.
[46] YAKHIN O I, LUBYANOV A A, YAKHIN I A.Changes in cytokinin, auxin, and abscisic acid contents in wheat seedlings treated with the growth regulator Stifun[J]. Russian Journal of Plant Physiology, 2012, 59(3): 398-405.
[47] ALONI R, ALONI E, LANGHANS M, et al.Role of cytokinin and auxin in shaping root architecture: regulating vascular differentiation, lateral root initiation, root apical dominance and root gravitropism[J]. Annals of Botany, 2006, 97(5): 883-893.
[48] SAMUELSON M E, LARSSON C M.Nitrate regulation of zeation riboside levels in barley roots: effects of inhibitors of N assimilation and comparison with ammonium[J]. Plant Science, 1993, 93(1/2): 77-84.

编辑推荐

Metrics

阅读次数

全文

2309

HTML			PDF

最新录用	在线预览	正式出版	最新录用	在线预览	正式出版
0	0	182	0	0	2127

来源	本网站	其他网站

次数	1614	695
比例	70%	30%

摘要

1062

最新录用	在线预览	正式出版

0	0	1062

来源	本网站	其他网站

次数	1052	10
比例	99%	1%

外源增钾缓解铵胁迫下小麦根系受抑

Potassium alleviates inhibition of ammonium stress on wheat root

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	韩晓蕾, 高仕祺, 张帆, 羊健, 刘芃, 姜鸿明, 李林志. 酵母双杂交筛选与小麦黄花叶病毒P2互作的寄主因子[J]. 浙江农业学报, 2021, 33(3): 497-505.
[2]	徐民民, 黄莹, 李波, 徐艳, 张帅, 姚岭芸, 王政. 生物炭对小麦根际和根内微生物群落结构的影响[J]. 浙江农业学报, 2021, 33(3): 516-525.
[3]	王潭刚, 孙婷, 王冀川, 李慧琴, 高振, 石元强. 播期和密度对滴灌冬小麦群体结构与抗倒特性的影响[J]. 浙江农业学报, 2021, 33(2): 193-202.
[4]	张统帅, 闫丽娟, 李广, 陈国鹏, 罗永忠. 免耕和秸秆覆盖对旱作区土壤氮素、水分和春小麦产量的影响[J]. 浙江农业学报, 2020, 32(8): 1329-1341.
[5]	岳建华, 董艳, 李文杨, 李蒙, 张琰. pH对百子莲体胚诱导期生理特性的影响[J]. 浙江农业学报, 2020, 32(8): 1405-1414.
[6]	张亮, 李玉婷, 许晓风. 锰离子胁迫下外生菌根真菌对土壤钾释放的影响[J]. 浙江农业学报, 2020, 32(7): 1215-1222.
[7]	吴承杰, 任兰天, 郝冰, 邵庆勤, 王泓, 陈峰, 代高峰, 梅世远, 张从军. 秸秆堆肥部分替代化肥配施硝化抑制剂对冬小麦温室气体排放的影响[J]. 浙江农业学报, 2020, 32(7): 1233-1240.
[8]	邱文怡, 王诗雨, 李晓芳, 徐恒, 张华, 朱英, 王良超. MYB转录因子参与植物非生物胁迫响应与植物激素应答的研究进展[J]. 浙江农业学报, 2020, 32(7): 1317-1328.
[9]	韩立杰, 董伟欣, 张月辰. 不同水肥处理对小麦冠层结构、产量和籽粒品质的影响[J]. 浙江农业学报, 2020, 32(6): 953-962.
[10]	徐莉, 陈小洁, 曹静婷, 刘楚楚, 丁婷, 江腾. 小麦赤霉病生防菌DZSG23的抗病机制[J]. 浙江农业学报, 2020, 32(11): 2001-2008.
[11]	边建文, 崔岩, 杨宋琪, 罗光宏, 孟宪刚. 衣藻和固氮鱼腥藻对盐胁迫下小麦幼苗生长的影响[J]. 浙江农业学报, 2020, 32(10): 1748-1756.
[12]	任嘉欣, 杨文娜, 李忠意, 胡艳燕. 基体效应对火焰光度计测定土壤和植株钾素含量准确性的影响[J]. 浙江农业学报, 2019, 31(6): 955-962.
[13]	王掌军, 刘妍, 张双喜, 刘凤楼, 李清峰, 张晓岗, 刘生祥, 贾彪. 宁春4号与河东乌麦杂交F₂代抗病性及分子标记鉴定[J]. 浙江农业学报, 2019, 31(5): 677-687.
[14]	陈小洁, 王其, 张欣悦, 丁婷. 杜仲内生细菌拮抗小麦赤霉病菌研究[J]. 浙江农业学报, 2019, 31(5): 766-776.
[15]	张岩, 亓玉华, 鲁燕华, 杨乾坤, 何雨娟, 李俊敏, 陈剑平. 小麦黄花叶病毒P3蛋白致病功能域的鉴定和分析[J]. 浙江农业学报, 2019, 31(5): 777-783.