宁春4号×河东乌麦F2∶5家系遗传图谱构建与籽粒蛋白质性状QTL分析

doi:10.3969/j.issn.1004-1524.2021.08.03

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (8): 1367-1378.DOI: 10.3969/j.issn.1004-1524.2021.08.03

宁春4号×河东乌麦F_2∶5家系遗传图谱构建与籽粒蛋白质性状QTL分析

王掌军¹(), 姚明明¹, 余慧霞¹, 王彦青¹, 李清峰¹, 刘凤楼¹, 刘彩霞¹, 张双喜², 张晓岗¹, 刘生祥¹

1.宁夏大学农学院,宁夏银川 750021
2.宁夏农林科学院农作物研究所,宁夏银川 750002

收稿日期:2020-10-19 出版日期:2021-08-25 发布日期:2021-08-27
作者简介:王掌军(1978—),男,甘肃天水人,博士,副教授,研究方向为小麦遗传育种。E-mail: wangzj-gs@126.com
基金资助:
宁夏自然科学基金(2021AAC03069);宁夏自然科学基金(NZ17126);宁夏回族自治区农业育种专项(2018NYYZ02)

Construction of genetic map and analysis of QTL for grain protein traits using F_2∶5 pedigrees derived from Ningchun No.4×Hedong black wheat

WANG Zhangjun¹(), YAO Mingming¹, YU Huixia¹, WANG Yanqing¹, LI Qingfeng¹, LIU Fenglou¹, LIU Caixia¹, ZHANG Shuangxi², ZHANG Xiaogang¹, LIU Shengxiang¹

1. Agricultural College, Ningxia University, Yinchuan 750021, China
2. Institute of Crop Sciences, Ningxia Academy of Agricultural-Forestry Sciences, Yinchuan 750002, China

Received:2020-10-19 Online:2021-08-25 Published:2021-08-27

摘要/Abstract

摘要：

为适应宁夏回族自治区小麦绿色优质高效品种选育和产业提质增效的需求,以宁春4号与河东乌麦杂交后代的248个F_2∶5家系为材料,对其进行遗传图谱构建与籽粒蛋白质数量性状基因座(quantitative trait locus,QTL)分析,以期为该地区小麦蛋白质性状遗传改良提供育种中间材料和QTL。结果表明:用197个SSR(simple sequence repeats)标记构建了包括小麦21条染色体的分子遗传图谱,总长度为2 342.63 cM,标记间平均距离为11.89 cM;蛋白质性状在F_2∶5家系出现较大分离,粗蛋白质含量、湿面筋含量的群体平均值(分别为14.49%、30.96%)介于双亲该性状之间,稳定时间的群体平均值(10.86 min)均超过高亲;粗蛋白质含量、稳定时间、湿面筋含量的超中亲比例分别达50.81%、77.82%、50.00%,超高亲比例分别达21.77%、59.27%、22.98%;籽粒蛋白质性状3个指标间均呈极显著正相关(P﹤0.01)。利用22个标记共检测到36个籽粒蛋白质QTL,分别为14个粗蛋白质含量QTL、6个稳定时间QTL和16个湿面筋含量QTL,涉及1A、2A、3A、5A、7A、1B、2B、6B、1D、2D、3D、4D、5D、6D、7D等15条染色体,36个QTL的连锁系数(LOD值)最大为14.9,表型贡献率为3%~6%,加性效应为-1.71~1.17,有11个标记所在的位点存在籽粒蛋白质性状QTL富集区。

关键词: 小麦, 家系, 遗传图谱, 蛋白质性状, 数量性状基因座

Abstract:

To adapt to the demand about green high quality and efficient cultivar breeding of wheat, and industry improving for Ningxia Hui Autonomous Region, using 248 F_2∶5 pedigrees of Ningchun No.4 × Hedong black wheat as materials, their genetic map was constructed, and grain protein traits QTL (quantitative trait locus) were analyzed, so as to provide breeding intermediate materials and QTL for genetic improvement of wheat protein traits in this area. The results showed that a molecular genetic map of 21 chromosomes in wheat was constructed using 197 SSR (simple sequence repeats) markers, the total length was 2 342.63 cM with an average interval distance of 11.89 cM. The distribution of quality traits in F_2∶5 pedigrees were significantly separated. Average values of crude protein content (14.49%) and wet gluten content (30.96%) were between those of high-parent and low-parent, the average value of stabilization time (10.86 min) was over the high parents. The proportion of over mid-parent was 50.81%, 77.82% and 50.00% in crude protein content, stabilization time and wet gluten content, respectively; Proportions of ultra-high parents was 21.77%, 59.27% and 22.98%, respectively. There was significantly positive correlation among three grain protein trait indices. 36 QTL loci of grain protein traits were explored by 22 SSR markers, there were 14 QTL, 6 QTL and 16 QTL of crude protein content, stabilization time and wet gluten content, respectively, those involved in chromosomes 1A, 2A, 3A, 5A, 7A, 1B, 2B, 6B, 1D, 2D, 3D, 4D, 5D, 6D and 7D, maximum logarithm of odds (LOD) score of these 36 QTL was 14.9, the contribution rates to phenotype were from 3% to 6%, their additive effects were from -1.71 to 1.17, and QTL enrichment regions of grain protein traits existed in 11 marker loci.

Key words: wheat (Triticum aestivum L.), pedigree, genetic map, protein trait, quantitative trait locus

中图分类号:

S512.1

王掌军, 姚明明, 余慧霞, 王彦青, 李清峰, 刘凤楼, 刘彩霞, 张双喜, 张晓岗, 刘生祥. 宁春4号×河东乌麦F_2∶5家系遗传图谱构建与籽粒蛋白质性状QTL分析[J]. 浙江农业学报, 2021, 33(8): 1367-1378.

WANG Zhangjun, YAO Mingming, YU Huixia, WANG Yanqing, LI Qingfeng, LIU Fenglou, LIU Caixia, ZHANG Shuangxi, ZHANG Xiaogang, LIU Shengxiang. Construction of genetic map and analysis of QTL for grain protein traits using F_2∶5 pedigrees derived from Ningchun No.4×Hedong black wheat[J]. Acta Agriculturae Zhejiangensis, 2021, 33(8): 1367-1378.

图/表 5

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引物编号 Marker name	染色体 Chromosome	位点 Loci	引物序列(5'-3') Primer sequence(5'-3')	退火温度 Annealing temperature/℃
Xwmc385	1A	Xwmc385-1A	GAAATGGCAATGGACTCAC CCATCATATCCCTAGCACAA	52
Xbarc137	1B	Xbarc137-1B	GGCCCATTTCCCACTTTCCA CCAGCCCCTCTACACATTTT	50
Xbarc181	1B	Xbarc181-1B	CGCTGGAGGGGGTAAGTCATCAC CGCAAATCAAGAACACGGGAGAAAGAA	50
Xcfd72	1D	Xcfd72-1D	CTCCTTGGAATCTCACCGAA TCCTTGGGAATATGCCTCCT	52
Xgwm249	2A	Xgwm249-2A	CAAATGGATCGAGAAAGGGA CTGCCATTTTTCTGGATCTACC	52
Xgwm372	2A	Xgwm372-2A	AATAGAGCCCTGGGACTGGG GAAGGACGACATTCCACCTG	50
Xbarc208	2A	Xbarc208-2A	GCGTGCTCATATTTCCAACATCAA GCGTGGTATAACAAAACTACACAACCTG	52
Xgpw2204	2A	Xgpw2204-2A	ACGTGCCTCGGTCGATATAC ACCCCGAGCATGTCAAATAG	60
XbarcM139	2B	XbarcM139-2B	AGAAGCTCCCCTAAACTGAG CGACGCTGATGAATGAAT	60
Xmag1241	2D	Xmag1241-2D	GGGACCAAAGCAAACACCATC GGCTACAAGGGTTCAAGATAATGC	60
Xbarc177	3A/5D	Xbarc177-3A	GCGATCCTGTTGTTGAGCGTTTGCATAA TCCCGTTTTCCCGTGTGTTAGTCTA	55
Xgdm8	3D	Xgdm8-3D	TTCTCCAACGCACGTTAGC CCCAAATGATGGCAGCTACT	55
Xgpw2220	4D	Xgpw2220-4D	TGACACCCCTTTCTTATCCG TAGCGAGGGGGTTAAGTGTG	55
Xwmc410	5A	Xwmc410-5A	GGACTTGAAAGGAAGCTTGTGA CATGGATGGCATGCAGTGT	55
Xbarc320	5D	Xbarc320-5D	CGTCTTCATCAAATCCGAACTG AAAATCTATGCGCAGGAGAAAC	55
Xbarc177	5D	Xbarc177-5D	GCGATCCTGTTGTTGAGCGTTTGCATAA TCCCGTTTTCCCGTGTGTTAGTCTA	60
XbarcM169	6B	XbarcM169-6B	CCGCGAACCATACAAAGGAAAC GCTATAGAGGCGCCTTGGAGTACC	55
Xcfb49	6D	Xcfb49-6D	TGAGTTCTTCTGGTGAGGCA GAATCGGTTCACAAGGGAAA	55
Xcfb13	6D	Xcfb13-6D	CCACTAACCAAGCTGCCATT TTTTTGGCATTGATCTGCTG	55
Xmag1518	7A	Xmag1518-7A	GCTAACTTCACCGACTTGACTACG ACTAAGCCACCCACTTGACC	55
Xmag1279	7A	Xmag1279-7A	TGGGCGTGACAACTGGGAG CATCCATAGCAGGACTTTCAATCC	55
Xgpw8188	7D	Xgpw8188-7D	CATGGTCAACGTCTGGGTG CTTGGAGAGTAGGACGGCAG	55

引物编号 Marker name	染色体 Chromosome	位点 Loci	引物序列(5'-3') Primer sequence(5'-3')	退火温度 Annealing temperature/℃
Xwmc385	1A	Xwmc385-1A	GAAATGGCAATGGACTCAC CCATCATATCCCTAGCACAA	52
Xbarc137	1B	Xbarc137-1B	GGCCCATTTCCCACTTTCCA CCAGCCCCTCTACACATTTT	50
Xbarc181	1B	Xbarc181-1B	CGCTGGAGGGGGTAAGTCATCAC CGCAAATCAAGAACACGGGAGAAAGAA	50
Xcfd72	1D	Xcfd72-1D	CTCCTTGGAATCTCACCGAA TCCTTGGGAATATGCCTCCT	52
Xgwm249	2A	Xgwm249-2A	CAAATGGATCGAGAAAGGGA CTGCCATTTTTCTGGATCTACC	52
Xgwm372	2A	Xgwm372-2A	AATAGAGCCCTGGGACTGGG GAAGGACGACATTCCACCTG	50
Xbarc208	2A	Xbarc208-2A	GCGTGCTCATATTTCCAACATCAA GCGTGGTATAACAAAACTACACAACCTG	52
Xgpw2204	2A	Xgpw2204-2A	ACGTGCCTCGGTCGATATAC ACCCCGAGCATGTCAAATAG	60
XbarcM139	2B	XbarcM139-2B	AGAAGCTCCCCTAAACTGAG CGACGCTGATGAATGAAT	60
Xmag1241	2D	Xmag1241-2D	GGGACCAAAGCAAACACCATC GGCTACAAGGGTTCAAGATAATGC	60
Xbarc177	3A/5D	Xbarc177-3A	GCGATCCTGTTGTTGAGCGTTTGCATAA TCCCGTTTTCCCGTGTGTTAGTCTA	55
Xgdm8	3D	Xgdm8-3D	TTCTCCAACGCACGTTAGC CCCAAATGATGGCAGCTACT	55
Xgpw2220	4D	Xgpw2220-4D	TGACACCCCTTTCTTATCCG TAGCGAGGGGGTTAAGTGTG	55
Xwmc410	5A	Xwmc410-5A	GGACTTGAAAGGAAGCTTGTGA CATGGATGGCATGCAGTGT	55
Xbarc320	5D	Xbarc320-5D	CGTCTTCATCAAATCCGAACTG AAAATCTATGCGCAGGAGAAAC	55
Xbarc177	5D	Xbarc177-5D	GCGATCCTGTTGTTGAGCGTTTGCATAA TCCCGTTTTCCCGTGTGTTAGTCTA	60
XbarcM169	6B	XbarcM169-6B	CCGCGAACCATACAAAGGAAAC GCTATAGAGGCGCCTTGGAGTACC	55
Xcfb49	6D	Xcfb49-6D	TGAGTTCTTCTGGTGAGGCA GAATCGGTTCACAAGGGAAA	55
Xcfb13	6D	Xcfb13-6D	CCACTAACCAAGCTGCCATT TTTTTGGCATTGATCTGCTG	55
Xmag1518	7A	Xmag1518-7A	GCTAACTTCACCGACTTGACTACG ACTAAGCCACCCACTTGACC	55
Xmag1279	7A	Xmag1279-7A	TGGGCGTGACAACTGGGAG CATCCATAGCAGGACTTTCAATCC	55
Xgpw8188	7D	Xgpw8188-7D	CATGGTCAACGTCTGGGTG CTTGGAGAGTAGGACGGCAG	55

性状 Trait	宁春4号 Ningchun No.4	河东乌麦 Hedongblack wheat	F_2∶5家系 F_2∶5 pedigree
性状 Trait	宁春4号 Ningchun No.4	河东乌麦 Hedongblack wheat	变异范围 Variationrange	平均值 Mean	标准差 Standard deviation	变异系数 Coefficient of variation/%	超中亲比例 Proportion of ultra-mid parent/%	超高亲比例 Proportion of ultra-high parent/%
粗蛋白质含量	13.61	15.28	11.25~17.82	14.49	0.15	1.04	50.81	21.77
Crude protein content/%
稳定时间	4.97	9.99	0.15~22.37	10.86	1.16	10.64	77.82	59.27
Stabilization time/min
湿面筋含量	29.25	32.77	22.84~38.49	30.96	0.40	1.29	50.00	22.98
Wet gluten content/%

性状 Trait	宁春4号 Ningchun No.4	河东乌麦 Hedongblack wheat	F_2∶5家系 F_2∶5 pedigree
性状 Trait	宁春4号 Ningchun No.4	河东乌麦 Hedongblack wheat	变异范围 Variationrange	平均值 Mean	标准差 Standard deviation	变异系数 Coefficient of variation/%	超中亲比例 Proportion of ultra-mid parent/%	超高亲比例 Proportion of ultra-high parent/%
粗蛋白质含量	13.61	15.28	11.25~17.82	14.49	0.15	1.04	50.81	21.77
Crude protein content/%
稳定时间	4.97	9.99	0.15~22.37	10.86	1.16	10.64	77.82	59.27
Stabilization time/min
湿面筋含量	29.25	32.77	22.84~38.49	30.96	0.40	1.29	50.00	22.98
Wet gluten content/%

性状 Traits	位点 Loci	标记位置 Marker position	标记名称 Marker name	LOD值 LOD value	表型贡献率 Phenotypic contribution rate/%	P值 P value	加性效应 Additive effect	显性效应 Dominant effect
粗蛋白质含量	qCPC1A	Chr1A	Xwmc385	14.9	6	0.000 6	-0.34	0.07
Crude protein	qCPC2A	Chr2A	Xgwm249	14.9	6	0.000 6	-0.34	0.07
content/%	qCPC3A	Chr3A	Xbarc177	14.9	6	0.000 6	-0.34	0.07
	qCPC5A	Chr5A	Xwmc410	9.5	4	0.008 6	0.29	0.28
	qCPC7A	Chr7A	Xmag1518	9.5	4	0.008 6	0.29	0.28
	qCPC1B	Chr1B	Xbarc137	9.5	4	0.008 6	0.29	0.28
	qCPC2B	Chr2B	XbarcM139	9.5	4	0.008 6	0.29	0.28
	qCPC2D	Chr2D	Xmag1241	12.5	5	0.001 9	-0.51	-0.28
	qCPC3D	Chr3D	Xgdm8	12.5	5	0.001 9	-0.51	-0.28
	qCPC4D	Chr4D	Xgpw2220	12.5	5	0.001 9	-0.51	-0.28
	qCPC5D	Chr5D	Xbarc320	12.5	5	0.001 9	-0.51	-0.28
	qCPC6D-1	Chr6D	Xcfd49	12.5	5	0.001 9	-0.51	-0.28
	qCPC6D-2	Chr6D	Xcfd13	14.9	6	0.000 6	-0.34	0.07
	qCPC7D	Chr7D	Xgpw8188	14.9	6	0.000 6	-0.34	0.07
稳定时间	qST2A-1	Chr2A	Xgwm372	6.8	3	0.033 6	0.79	0.72
Stabilization	qST2A-2	Chr2A	Xbarc208	8.2	3	0.016 3	0.90	0.67
time/min	qST2A-3	Chr2A	Xgpw2204	10.9	4	0.004 2	1.17	0.70
	qST3A	Chr3A	Xbarc177	12.2	5	0.002 2	-1.14	0
	qST5D	Chr5D	Xbarc177	12.2	5	0.002 2	-1.14	0
	qST6D	Chr6D	Xcfd49	11.4	5	0.003 3	-1.71	-0.69
湿面筋含量	qWGC1A	Chr1A	Xwmc385	14.0	5	0.000 9	-0.79	0.20
Wet gluten	qWGC2A	Chr2A	Xgwm249	14.0	5	0.000 9	-0.79	0.20
content/%	qWGC3A	Chr3A	Xbarc177	14.0	5	0.000 9	-0.79	0.20
	qWGC5A	Chr5A	Xwmc410	7.7	3	0.021 6	0.54	0.64
	qWGC7A	Chr7A	Xmag1279	7.1	3	0.029 4	-0.57	-0.53
	qWGC1B	Chr1B	Xbarc181	7.1	3	0.029 4	-0.57	-0.53
	qWGC2B	Chr2B	XbarcM139	7.7	3	0.021 6	0.54	0.64
	qWGC6B	Chr6B	XbarcM169	6.5	3	0.038 4	0.36	-0.79
	qWGC1D	Chr1D	Xcfd72	6.5	3	0.038 4	0.36	-0.79
	qWGC2D	Chr2D	Xmag1241	11.9	5	0.002 7	-1.22	-0.75
	qWGC3D	Chr3D	Xgdm8	11.9	5	0.002 7	-1.22	-0.75
	qWGC4D	Chr4D	Xgpw2220	11.9	5	0.002 7	-1.22	-0.75
	qWGC5D	Chr5D	Xbarc320	11.9	5	0.002 7	-1.22	-0.75
	qWGC6D-1	Chr6D	Xcfd49	11.9	5	0.002 7	-1.22	-0.75
	qWGC6D-2	Chr6D	Xcfd13	14.0	5	0.000 9	-0.79	0.20
	qWGC7D	Chr7D	Xgpw8188	14.0	5	0.000 9	-0.79	0.20

宁春4号×河东乌麦F_2∶5家系遗传图谱构建与籽粒蛋白质性状QTL分析

Construction of genetic map and analysis of QTL for grain protein traits using F_2∶5 pedigrees derived from Ningchun No.4×Hedong black wheat

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宁春4号×河东乌麦F2∶5家系遗传图谱构建与籽粒蛋白质性状QTL分析

Construction of genetic map and analysis of QTL for grain protein traits using F2∶5 pedigrees derived from Ningchun No.4×Hedong black wheat

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宁春4号×河东乌麦F_2∶5家系遗传图谱构建与籽粒蛋白质性状QTL分析

Construction of genetic map and analysis of QTL for grain protein traits using F_2∶5 pedigrees derived from Ningchun No.4×Hedong black wheat